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当前位置： 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-iNOS Antibody/SPC-1325D-A488/100-µl
商品详细StressMarq/Anti-iNOS Antibody/SPC-1325D-A488/100-µl
StressMarq/Anti-iNOS Antibody/SPC-1325D-A488/100-µl
StressMarq/Anti-iNOS Antibody/SPC-1325D-A488/100-µl
商品编号： SPC-1325D-A488
市场价： ¥6040.00
美元价： 3624.00
产地： 美国(厂家直采)
公司：
产品分类： 酸碱缓冲液
公司分类： acid_base_buffer_solution
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name iNOS Antibody
Description

Rabbit Anti-Human iNOS Polyclonal

Species Reactivity Human
Applications WB, IHC, ICC/IF, ELISA
Antibody Dilution WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthesized peptide derived from human iNOS.
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Isotype IgG
Specificity Detects endogenous levels of total iNOS protein.
Cite This Product StressMarq Biosciences Cat# SPC-1325, RRID: AB_2713637
Certificate of Analysis A 1:1000 dilution of SPC-1325 was sufficient for detection of iNOS in 10 µg of NIH/3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names HEP-NOS antibody, Hepatocyte NOS antibody, HEPNOS antibody, inducible antibody, Inducible nitric oxide synthase antibody, Inducible NO synthase antibody, Inducible NOS antibody, iNOS antibody, MAC NOS antibody, Macrophage NOS antibody, Nitric oxide synthase 2 inducible antibody, Nitric oxide synthase 2 inducible macrophage antibody, nitric oxide synthase 2A (inducible, hepatocytes) antibody, Nitric oxide synthase antibody, Nitric oxide synthase inducible antibody, nitric oxide synthase, macrophage antibody, NOS 2 antibody, NOS antibody, Nos II antibody, NOS type II antibody, nos2 antibody, NOS2_HUMAN antibody, NOS2A antibody, NOS2A, Inducible, Hepatocyte antibody, Peptidyl-cysteine S-nitrosylase NOS2 antibody
Cellular Localization Cytoplasm, Nucleus, Peroxisome
Accession Number NP_000616.3
Gene ID 4843
Swiss Prot P35228
Scientific Background Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned.
References 1. Knowles RG and Moncada S. (1994) Biochem. J. 298 (2): 249–58.
2. Burger DE. et al. (2009) Circulation 120 (14): 1345–54.
3. Taylor BS. (1997) Arch Surg 132 (11): 1177–83.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Tissue: HepG2 cells. Species: Human. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Tissue: HepG2 cells. Species: Human. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~131kDa iNOS protein using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:1000. Predicted/Observed Size: ~131kDa.</p>

Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~131kDa iNOS protein using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:1000. Predicted/Observed Size: ~131kDa.

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  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

  ATTO 488 Fluorophore Excitation and Emission SpectraOptical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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