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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-iNOS Antibody/SPC-1325D-A390/100-µl
商品详细StressMarq/Anti-iNOS Antibody/SPC-1325D-A390/100-µl
StressMarq/Anti-iNOS Antibody/SPC-1325D-A390/100-µl
StressMarq/Anti-iNOS Antibody/SPC-1325D-A390/100-µl
商品编号: SPC-1325D-A390
市场价: ¥6060.00
美元价: 3636.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameiNOSAntibody
Description

RabbitAnti-HumaniNOSPolyclonal

SpeciesReactivityHuman
ApplicationsWB,IHC,ICC/IF,ELISA
AntibodyDilutionWB(1:1000),IHC(1:100),ICC/IF(1:500),ELISA(1:20000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenSynthesizedpeptidederivedfromhumaniNOS.
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityPolyclonal
IsotypeIgG
SpecificityDetectsendogenouslevelsoftotaliNOSprotein.
CiteThisProductStressMarqBiosciencesCat#SPC-1325,RRID:AB_2713637
CertificateofAnalysisA1:1000dilutionofSPC-1325wassufficientfordetectionofiNOSin10µgofNIH/3T3celllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesHEP-NOSantibody,HepatocyteNOSantibody,HEPNOSantibody,inducIBLeantibody,Induciblenitricoxidesynthaseantibody,InducibleNOsynthaseantibody,InducibleNOSantibody,iNOSantibody,MACNOSantibody,MacrophageNOSantibody,Nitricoxidesynthase2inducibleantibody,Nitricoxidesynthase2induciblemacrophageantibody,nitricoxidesynthase2A(inducible,hepatocytes)antibody,Nitricoxidesynthaseantibody,Nitricoxidesynthaseinducibleantibody,nitricoxidesynthase,macrophageantibody,NOS2antibody,NOSantibody,NosIIantibody,NOStypeIIantibody,nos2antibody,NOS2_HUMANantibody,NOS2Aantibody,NOS2A,Inducible,Hepatocyteantibody,Peptidyl-cysteineS-nitrosylaseNOS2antibody
CellularLocalizationCytoplasm,Nucleus,Peroxisome
AccessionNumberNP_000616.3
GeneID4843
SwissProtP35228
ScientificBackgroundNitricoxide(NO)isaninorganic,gaseousfreerADIcalthatcarriesavarietyofmessagesbetweencells.Vasorelaxation,neurotransmissionandcytotoxicitycanallbepotentiatedthroughcellularresponsetoNO.NOproductionismediatedbymembersofthenitricoxidesynthase(NOS)family.NOScatalyzestheoxidizationofL-argininetoproduceL-citrullineandNO.Twoconstitutiveisoforms,brainorneuronalNOS(bornNOS,typeI)&endothelialcellNOS(eNOS,typeIII),andoneinducibleisoform(iNOS,typeII),havebeencloned.
References1.KnowlesRGandMoncadaS.(1994)Biochem.J.298(2):249–58.
2.BurgerDE.etal.(2009)Circulation120(14):1345–54.
3.TaylorBS.(1997)ArchSurg132(11):1177–83.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:100. The image on the right is treated with the synthesized peptide.</p>

ImmunohistochemistryanalysisusingRabbitAnti-iNOSPolyclonalAntibody(SPC-1325).Tissue:LungCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-iNOSPolyclonalAntibody(SPC-1325)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Tissue: HepG2 cells. Species: Human. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-iNOSPolyclonalAntibody(SPC-1325).Tissue:HepG2cells.Species:Human.PrimaryAntibody:RabbitAnti-iNOSPolyclonalAntibody(SPC-1325)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~131kDa iNOS protein using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:1000. Predicted/Observed Size: ~131kDa.</p>

WesternblotanalysisofMouseNIH/3T3celllysatesshowingdetectionof~131kDaiNOSproteinusingRabbitAnti-iNOSPolyclonalAntibody(SPC-1325).Lane1:MouseNIH/3T3.Lane2:MouseNIH/3T3treatedwiththeimmunizingpeptide.PrimaryAntibody:RabbitAnti-iNOSPolyclonalAntibody(SPC-1325)at1:1000.Predicted/ObservedSize:~131kDa.

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 ATTO390
Overview:
  • Highfluorescenceyield
  • LargeStokes-shift(89nm)
  • GoodphotostABIlity
  • Moderatelyhydrophilic
  • Goodsolubilityinpolarsolvents
  • Coumarinderivate,uncharged
  • Lowmolarmass:343.42g/mol 

ATTO390Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission SpectraOpticalProperties:

λex =390nm

λem=479nm

εmax=2.4×104

Φf= 0.90

τfl =5.0ns

Brightness= 21.6

Laser =365or405nm

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。