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当前位置： 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-HSP90 beta Antibody (Ser254)/SPC-1266D/100-µl
商品详细StressMarq/Anti-HSP90 beta Antibody (Ser254)/SPC-1266D/100-µl
StressMarq/Anti-HSP90 beta Antibody (Ser254)/SPC-1266D/100-µl
StressMarq/Anti-HSP90 beta Antibody (Ser254)/SPC-1266D/100-µl
商品编号： SPC-1266D
市场价： ¥5100.00
美元价： 3060.00
产地： 美国(厂家直采)
公司：
产品分类： 酸碱缓冲液
公司分类： acid_base_buffer_solution
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name HSP90 beta Antibody (Ser254)
Description

Rabbit Anti-Human HSP90 beta (Ser254) Polyclonal

Species Reactivity Human, Mouse, Rat
Applications WB, IHC, IP, ICC/IF, ELISA
Antibody Dilution WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:40000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthesized unphosphorylated peptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E).
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Isotype IgG
Specificity Detects endogenous levels of total HSP90B protein.
Cite This Product StressMarq Biosciences Cat# SPC-1266, RRID: AB_2712593
Certificate of Analysis A 1:1000 dilution of SPC-1266 was sufficient for detection of Hsp90 beta in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names HSP84 Antibody, HSP90 Antibody, HSP86 Antibody, HSP89 Antibody, HSP90B Antibody, HSP90BETA Antibody, HSP90N Antibody, HSPC2 Antibody, HSPCA Antibody, HSPCAL1 Antibody, HSPCB Antibody, HSPN Antibody, LAP2 Antibody, NY REN 38 antigen Antibody
Research Areas Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Chaperones, Protein Trafficking, Trafficking
Cellular Localization Cytoplasm, Melanosome
Accession Number NP_031381.2
Gene ID 3326
Swiss Prot P08238
Scientific Background HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85% sequence amino acid homology. The two isoforms of HSP90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer.(2) From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, HSP90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it’s label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling. (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9). Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at http://www.HSP90.ca.
References 1. Nemoto T., et al. (1997) J.Biol Chem. 272: 26179-26187.
2. Minami Y., et al. (1991), J.Biol Chem. 266: 10099-10103.
3. Arlander S.J.H., et al. (2003) J Biol Chem 278: 52572-52577.
4. Pearl H., et al. (2001) Adv Protein Chem 59:157-186.
5. Neckers L., et al. (2002) Trends Mol Med 8:S55-S61.
6. Pratt W., Toft D. (2003) Exp Biol Med 228:111-133.
7. Pratt W., Toft D. (1997) Endocr Rev 18:306–360.
8. Pratt W.B. (1998) Proc Soc Exptl Biol Med 217: 420–434.
9. Whitesell L., et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328.
10. Barent R. L. (1998) Mol. Endocrinol. 12: 342-354
11. Lo. M.A. (1998) EMBO J. 17: 6879-6887.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-Hsp90 beta Polyclonal Antibody (SPC-1266). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Hsp90 beta Polyclonal Antibody (SPC-1266) at 1:1000. The image on the right is treated with the synthesized phosphorylated peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-Hsp90 beta Polyclonal Antibody (SPC-1266). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Hsp90 beta Polyclonal Antibody (SPC-1266) at 1:1000. The image on the right is treated with the synthesized phosphorylated peptide.

<p>Western blot analysis of Human HeLa cell lysates showing detection of ~90kDa Hsp90 beta protein using Rabbit Anti-Hsp90 beta Polyclonal Antibody (SPC-1266). Lane 1: Human HeLa cells treated with TNF-? (20ng/ml, 30mins). Lane 2: Untreated. Primary Antibody: Rabbit Anti-Hsp90 beta Polyclonal Antibody (SPC-1266) at 1:1000. Predicted/Observed Size: ~90kDa.</p>

Western blot analysis of Human HeLa cell lysates showing detection of ~90kDa Hsp90 beta protein using Rabbit Anti-Hsp90 beta Polyclonal Antibody (SPC-1266). Lane 1: Human HeLa cells treated with TNF-? (20ng/ml, 30mins). Lane 2: Untreated. Primary Antibody: Rabbit Anti-Hsp90 beta Polyclonal Antibody (SPC-1266) at 1:1000. Predicted/Observed Size: ~90kDa.

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StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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