StressMarq/Anti-HSP90 beta Antibody (pSer254)/SPC-1267D-A390/100-µl-免疫在线蚂蚁淘旗下平台

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商品详细StressMarq/Anti-HSP90 beta Antibody (pSer254)/SPC-1267D-A390/100-µl

StressMarq/Anti-HSP90 beta Antibody (pSer254)/SPC-1267D-A390/100-µl

商品编号： SPC-1267D-A390

品牌： StressMarq Biosciences

市场价： ¥6060.00

美元价： 3636.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName HSP90betaAntibody(pSer254)

Description

RabbitAnti-HumanHSP90beta(pSer254)Polyclonal

SpeciesReactivity Human,Mouse,Rat

Applications WB,IHC,ICC/IF,ELISA

AntibodyDilution WB(1:1000),IHC(1:100),ICC/IF(1:500),ELISA(1:40000);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Rabbit

ImmunogenSpecies Human

Immunogen SynthesizedphosphopeptidederivedfromhumanHSP90Baroundthephosphorylationsiteofserine254(V-G-SP-D-E).

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBuffer	PBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	AffinityPurified
Clonality	Polyclonal
Isotype	IgG
Specificity	DetectsendogenouslevelsofHSP90Bonlywhenphosphorylatedatserine254.
CiteThisProduct	StressMarqBiosciencesCat#SPC-1267,RRID:AB_2712611
CertificateofAnalysis	A1:1000dilutionofSPC-1267wassufficientfordetectionofHsp90betain10µgofHeLacelllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	HSP84Antibody,HSP90Antibody,HSP86Antibody,HSP89Antibody,HSP90BAntibody,HSP90BETAAntibody,HSP90NAntibody,HSPC2Antibody,HSPCAAntibody,HSPCAL1Antibody,HSPCBAntibody,HSPNAntibody,LAP2Antibody,NYREN38antigenAntibody
ResearchAreas	Cancer,HeatShock,CellSignaling,ChaperoneProteins,Chaperones,ProteinTrafficking,Trafficking
CellularLocalization	Cytoplasm,Melanosome
AccessionNumber	NP_031381.2
GeneID	3326
SwissProt	P08238
ScientificBackground	HSP90isanabundantlyandubiquitouslyexpressedheatshockprotein.Itisunderstoodtoexistintwoprincipalformsαandβ,whichshare85%sequenceaminoacidhomology.ThetwoisoformsofHSP90,areexpressedinthecytosoliccompartment(1).Despitethesimilarities,HSP90αexistspredominantlyasahomodimerwhileHSP90βexistsmainlyasamonomer.(2)Fromafunctionalperspective,HSP90participatesinthefolding,assembly,maturation,andstABIlizationofspecificproteinsasanintegralcomponentofachaperonecomplex.(3-6)FurThermore,HSP90ishighlyconservedbetweenspecies;having60%and78%aminoacidsimilaritybetweenmammalianandthecorrespondingyeastandDrosophilaproteins,respectively.HSP90isahighlyconservedandessentialstressproteinthatisexpressedinalleukaryoticcells.Despiteit’slabelofbeingaheat-shockprotein,HSP90isoneofthemosthighlyexpressedproteinsinunstressedcells(1–2%ofcytosolicprotein).Itcarriesoutanumberofhousekeepingfunctions–includingcontrollingtheactivity,turnover,andtraffickingofavarietyofproteins.MostoftheHSP90-regulatedproteinsthathavebeendiscoveredtodateareinvolvedincellsignaling.(7-8).ThenumberofproteinsnowknowtointeractwithHSP90isabout100.Targetproteinsincludethekinasesv-Src,Wee1,andc-Raf,transcriptionalregulatorssuchasp53andsteroidreceptors,andthepolymerasesofthehepatitisBvirusandtelomerase.5WhenboundtoATP,HSP90interactswithco-chaperonesCdc37,p23,andanassortmentofimmunophilin-likeproteins,formingacomplexthatstabilizesandprotectstargetproteinsfromproteasomaldegradation.Inmostcases,HSP90-interactingproteinshavebeenshowntoco-precipitatewithHSP90whencarryingoutimmunoadsorptionstudies,andtoexistincytosolicheterocomplexeswithit.Inanumberofcases,variationsinHSP90expressionorHSP90mutationhasbeenshowntodegradesignalingfunctionviatheproteinortoimpairaspecificfunctionoftheprotein(suchassteroidbinding,kinaseactivity)invivo.Ansamycinantibiotics,suchasgeldanamycinandrADIcicol,inhibitHSP90function(9).LookingformoreinformationonHSP90?VisitournewHSP90ScientificResourceGuideathttp://www.HSP90.ca.
References	1.NemotoT.,etal.(1997)J.BiolChem.272:26179-26187. 2.MinamiY.,etal.(1991),J.BiolChem.266:10099-10103. 3.ArlanderS.J.H.,etal.(2003)JBiolChem278:52572-52577. 4.PearlH.,etal.(2001)AdvProteinChem59:157-186. 5.NeckersL.,etal.(2002)TrendsMolMed8:S55-S61. 6.PrattW.,ToftD.(2003)ExpBiolMed228:111-133. 7.PrattW.,ToftD.(1997)EndocrRev18:306–360. 8.PrattW.B.(1998)ProcSocExptlBiolMed217:420–434. 9.WhitesellL.,etal.(1994)ProcNatlAcadSciUSA91:8324–8328. 10.BarentR.L.(1998)Mol.Endocrinol.12:342-354 11.Lo.M.A.(1998)EMBOJ.17:6879-6887.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-Hsp90 beta Polyclonal Antibody (SPC-1267). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Hsp90 beta Polyclonal Antibody (SPC-1267) at 1:1000. The image on the right is treated with the synthesized phosphorylated peptide.</p>

ImmunohistochemistryanalysisusingRabbitAnti-Hsp90betaPolyclonalAntibody(SPC-1267).Tissue:BreastCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-Hsp90betaPolyclonalAntibody(SPC-1267)at1:1000.Theimageontherightistreatedwiththesynthesizedphosphorylatedpeptide.

<p>Western blot analysis of Human HeLa cell lysates showing detection of ~90kDa Hsp90 beta protein using Rabbit Anti-Hsp90 beta Polyclonal Antibody (SPC-1267). Lane 1: Human HeLa cells treated with TNF-? (20ng/ml, 30mins). Lane 2: Untreated. Primary Antibody: Rabbit Anti-Hsp90 beta Polyclonal Antibody (SPC-1267) at 1:1000. Predicted/Observed Size: ~90kDa.</p>

WesternblotanalysisofHumanHeLacelllysatesshowingdetectionof~90kDaHsp90betaproteinusingRabbitAnti-Hsp90betaPolyclonalAntibody(SPC-1267).Lane1:HumanHeLacellstreatedwithTNF-?(20ng/ml,30mins).Lane2:Untreated.PrimaryAntibody:RabbitAnti-Hsp90betaPolyclonalAntibody(SPC-1267)at1:1000.Predicted/ObservedSize:~90kDa.

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ATTO390

Overview:

Highfluorescenceyield
LargeStokes-shift(89nm)
Goodphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
Coumarinderivate,uncharged
Lowmolarmass:343.42g/mol

ATTO390Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

OpticalProperties:

λ_ex =390nm

λ_em=479nm

ε_max=2.4×10⁴

Φ_f= 0.90

τ_fl =5.0ns

Brightness= 21.6

Laser =365or405nm

品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

公司介绍