StressMarq/Anti-HSP90 Antibody [H9010]/SMC-107B-A488/200-µg-免疫在线蚂蚁淘旗下平台

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商品详细StressMarq/Anti-HSP90 Antibody [H9010]/SMC-107B-A488/200-µg

StressMarq/Anti-HSP90 Antibody [H9010]/SMC-107B-A488/200-µg

商品编号： SMC-107B-A488

品牌： StressMarq Biosciences

市场价： ¥7920.00

美元价： 4752.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

Product Name HSP90 Antibody

Description

Mouse Anti-Human HSP90 Monoclonal IgG2a

Species Reactivity Dog, Human, Mouse, Rat, Chicken, Fish, Gummy Shark (Mustelus antarcticus), Rabbit, School Shark (Galeorhinus galeus), Shark, White Sucker Fish (Catostomus commersonii)

Applications WB, IHC, ICC/IF, IP, ELISA, AM

Antibody Dilution WB (1:2500), IHC (1:100); optimal dilutions for assays should be determined by the user.

Host Species Mouse

Immunogen Species Human

Immunogen Recombinant human HSP90beta

Concentration 1 mg/ml

Conjugates

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-Protein Complex
Small phycobiliprotein
Isolated from red algae
Large stokes shift (195 nm)
Molecular Weight: 35 kDa

PerCP Datasheet

PerCP Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 482 nm

λ_em = 677 nm

ε_max = 1.96 x 10⁶

Laser = 488 nm

PE/ATTO 594

PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation

PE/ATTO 594 Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

Optical Properties:

λ_ex = 535 nm

λ_em = 627 nm

Laser = 488 to 561 nm

FITC (Fluorescein)

Overview:

Excellent fluorescence quantum yield
High rate of photobleaching
Good solubility in water
Broad emission spectrum
pH dependent spectra
Molecular formula: C₂₀H₁₂O₅
Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 494 nm

λ_em = 520 nm

ε_max = 7.3×10⁴

Φ_f = 0.92

τ_fl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

ATTO 700

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 575 g/mol

ATTO 700 Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 700 nm

λ_em = 719 nm

ε_max = 1.25×10⁵

Φ_f = 0.25

τ_fl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

ATTO 680

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 631 g/mol

ATTO 680 Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 680 nm

λ_em = 700 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

ATTO 655

Overview:

High fluorescence yield
High thermal and photostability
Excellent ozone resistance
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 634 g/mol

ATTO 655 Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

ATTO 633

Overview:

High fluorescence yield
High thermal and photostability
Moderately hydrophilic
Good solubility in polar solvents
Stable at pH 4 – 11
Cationic dye, perchlorate salt
Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 629 nm

λ_em = 657 nm

ε_max = 1.3×10⁵

Φ_f = 0.64

τ_fl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

ATTO 594

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 1137 g/mol

ATTO 594 Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

Optical Properties:

λ_ex = 601 nm

λ_em = 627 nm

ε_max = 1.2×10⁵

Φ_f = 0.85

τ_fl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

ATTO 565

Overview:

High fluorescence yield
High thermal and photostability
Good solubility in polar solvents
Excellent solubility in water
Very little aggregation
Rhodamine dye derivative
Molar Mass: 611 g/mol

ATTO 565 Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 563 nm

λ_em = 592 nm

ε_max = 1.2×10⁵

Φ_f = 0.9

τ_fl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

ATTO 488

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 804 g/mol

ATTO 488 Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

ATTO 390

Overview:

High fluorescence yield
Large Stokes-shift (89 nm)
Good photostability
Moderately hydrophilic
Good solubility in polar solvents
Coumarin derivate, uncharged
Low molar mass: 343.42 g/mol

ATTO 390 Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

Optical Properties:

λ_ex = 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f = 0.90

τ_fl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

APC (Allophycocyanin)

Overview:

High quantum yield
Large phycobiliprotein
6 chromophores per molecule
Isolated from red algae
Molecular Weight: 105 kDa

APC Datasheet

APC Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 650 nm

λ_em = 660 nm

ε_max = 7.0×10⁵

Φ_f = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

Streptavidin

Properties:

Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
Molecular weight: 53 kDa
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

HRP (Horseradish peroxidase)

Properties:

Enzymatic activity is used to amplify weak signals and increase visibility of a target
Readily combines with hydrogen peroxide (H₂O₂) to form HRP-H₂O₂ complex which can oxidize various hydrogen donors
Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
High turnover rate enables rapid generation of a strong signal
44 kDa glycoprotein
Extinction coefficient: 100 (403 nm)
Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

AP (Alkaline Phosphatase)

Properties:

Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
Molecular weight: 140 kDa
Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

R-PE (R-Phycoerythrin)

Overview:

Broad excitation spectrum
High quantum yield
Photostable
Member of the phycobiliprotein family
Isolated from red algae
Excellent solubility in water
Molecular Weight: 250 kDa

R-PE Datasheet

R-PE Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 565 nm

λ_em = 575 nm

ε_max = 2.0×10⁶

Φ_f = 0.84

Brightness = 1.68 x 10³

Laser = 488 to 561 nm

Filter set = TRITC

Properties

Storage Buffer	PBS pH7.2, 50% glycerol, 0.09% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Protein G Purified
Clonality	Monoclonal
Clone Number	H9010
Isotype	IgG2a
Specificity	Detects 90kDa. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.
Cite This Product	StressMarq Biosciences Cat# SMC-107, RRID: AB_854214
Certificate of Analysis	1 µg/ml of SMC-107 was sufficient for detection of HSP90beta in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names	HSP84 Antibody, HSP90 Antibody, HSP90 beta Antibody, HSP90B Antibody, HSPC2 Antibody, HSPCB Antibody
Research Areas	Cancer, Heat Shock
Cellular Localization	Cytoplasm, Melanosome
Accession Number	NP_031381.2
Gene ID	3326
Swiss Prot	P08238
Scientific Background	HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85% sequence amino acid homology. The two isoforms of HSP90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer (2). From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (3-6). Furthermore, HSP90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it’s label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.
References	1. Nemoto T., et al. (1997) J.Biol Chem. 272: 26179-26187. 2. Minami Y., et al. (1991), J.Biol Chem. 266: 10099-10103. 3. Arlander S.J.H., et al. (2003) J Biol Chem 278: 52572-52577. 4. Pearl H., et al. (2001) Adv Protein Chem 59:157-186. 5. Neckers L., et al. (2002) Trends Mol Med 8:S55-S61. 6. Pratt W., Toft D. (2003) Exp Biol Med 228:111-133. 7. Pratt W., Toft D. (1997) Endocr Rev 18:306–360. 8. Pratt W.B. (1998) Proc Soc Exptl Biol Med 217: 420–434. 9. Whitesell L., et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328. 10. Barent R. L. (1998) Mol. Endocrinol. 12: 342-354 11. Lo. M.A. (1998) EMBO J. 17: 6879-6887.

Product Images

<p>Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x.</p>

Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x.

<p>Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.</p>

Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

<p>Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.</p>

Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x.

Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

<p>Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.</p>

Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

<p>Western Blot analysis of Human HeLa cell lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.</p>

Western Blot analysis of Human HeLa cell lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.

<p>Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.</p>

Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (SMC-107). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-107) at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.

Product Citations (24)

Western Blot

PRAS40 Connects Microenvironmental Stress Signaling to Exosome-Mediated Secretion.

Guo, J. et al. (2017) Mol Cell Biol. [Epub ahead of print].

PubMed ID: 28674187 Reactivity Human Applications: Western Blot

Extracellular heat shock protein 90α mediates HDM-induced bronchial epithelial barrier dysfunction by activating RhoA/MLC signaling.

Dong, H.M. et al. (2017) Respir Res. 18(1):111.

PubMed ID: 28558721 Reactivity Human Applications: Western Blot

Extracellular Hsp90 and TGFβ regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model.

de la Mare, J.A., Jurgens, T. and Edkins, A.L. (2017) BMC Cancer. 17(1):202.

PubMed ID: 28302086 Reactivity Human Applications: Western Blot

Other Citations

Characterization and Functional Analysis of Extracellular Vesicles and Muscle-Abundant miRNAs (miR-1, miR-133a, and miR-206) in C2C12 Myocytes and mdx Mice.

Matsuzaka, Y. et al. (2016) PLoS One. 11(12):e0167811.

PubMed ID: 27977725 Reactivity Mouse Applications: Western blot

Evolutionarily conserved dual lysine motif determines the non-chaperone function of secreted Hsp90alpha in tumour progression.

Zou, M. et al. (2016) Oncogene. 36(15):2160-2171.

PubMed ID: 27721406 Reactivity Human Applications: Western Blot

The epichaperome is an integrated chaperome network that facilitates tumour survival.

Rodina, A. et al. (2016) Nature. 538(7625):397-401.

PubMed ID: 27706135 Reactivity Human Applications: Western Blot

Physiological responses to hypersalinity correspond to nursery ground usage in two inshore shark species (Mustelus antarcticus & Galeorhinus galeus).

Tunnah, L. et al. (2016) J Exp Biol. 219(Pt 13):2028-38.

PubMed ID: 27207636 Reactivity Shark Applications: Western Blot

COMPOSITIONS AND METHODS FOR TREATING HIF-1a OVER-EXPRESSING CANCERS.

Li, W. et al. (2016) United States Patent Application 20160053003

PubMed ID: N/A Reactivity Human Applications: Western Blot

Breast Cancer MDA-MB-231 Cells Use Secreted Heat Shock Protein-90alpha (Hsp90α) to Survive a Hostile Hypoxic Environment.

Dong, H. et al. (2016) Sci Rep. 6:20605.

PubMed ID: 26846992 Reactivity Human Applications: Western Blot

Pharmacoproteomics identifies combinatorial therapy targets for diffuse large B cell lymphoma.

Goldstein, R.L. et al. (2015) J Clin Invest. 2015. pii: 80714

PubMed ID: 26529251 Reactivity Human Applications: Western Blot

Cold acclimation increases levels of some heat shock protein and sirtuin isoforms in threespine stickleback.

Teigen, L.E., Orczewska, J.I., McLaughlin, J. (2015) Comp Biochem Physiol A Mol Integr Physiol. 188:139-47.

PubMed ID: 26123780 Reactivity Gasterosteus aculeatus (Three-spined stickleback) Applications: Western Blot

Hsp90α and Hsp90β together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing.

Jayaprakash, P. et al. (2015) J Cell Sci. 128(8):1475-80.

PubMed ID: 25736295 Reactivity Human Applications: Western Blot

Cardiorespiratory toxicity of environmentally relevant zinc oxide nanoparticles in the freshwater fish Catostomus commersonii.

Bessemer, R.A. et al. (2014) Nanotoxicology. 27:1-10.

PubMed ID: 25427894 Reactivity Catostomus commersonii (fish) Applications: Western Blot

Hsp90 Binds Directly to Fibronectin (FN) and Inhibition Reduces the Extracellular Fibronectin Matrix in Breast Cancer Cells.

Hunter, M. C. et al. (2014) PLoS One. 9(1):e86842.

PubMed ID: 24466266 Reactivity Human Applications: Western Blot

Uses of Labeled HSP90 Inhibitors.

Chiosis, G. et al. (2014) United States Patent Application 20140294725 Kind Code: A1

PubMed ID: N/A Reactivity Human Applications: Western Blot

Paralog-selective Hsp90 inhibitors define tumor-specific regulation of HER 2.

Patel, P.D. et al. (2013) Nat Chem Biol. 9(11):677-84.

PubMed ID: 23995768 Reactivity Human Applications: Western Blot

A Novel Neurotrophic Drug for Cognitive Enhancement and Alzheimer's Disease.

Chen, Q. et al. (2011) PLoS One. 6 (12): e27865.

PubMed ID: 22194796 Reactivity Rat Applications: Western Blot

Design, synthesis, and evaluation of small molecule Hsp90 probes.

Taldone, T. et al. (2011) Bioorg Med Chem. 19 (8): 2603-2614.

PubMed ID: 21459002 Reactivity Human Applications: Western Blot

Key motifs in EBV (Epstein-Barr virus)-encoded protein kinase for phosphorylation activity and nuclear localization.

Gershburg, S., Murphy, L., Marschall, M. and Gershburg, E. (2010) Biochem J. 431 (2): 227-235.

PubMed ID: 20704565 Reactivity Human Applications: Western Blot

The epichaperome is an integrated chaperome network that facilitates tumour survival.

Rodina, A. et al. (2016) Nature. 538(7625):397-401.

PubMed ID: 27706135 Reactivity Human Applications: Immunocytochemistry/Immunofluorescence

Breast Cancer MDA-MB-231 Cells Use Secreted Heat Shock Protein-90alpha (Hsp90α) to Survive a Hostile Hypoxic Environment.

Dong, H. et al. (2016) Sci Rep. 6:20605.

PubMed ID: 26846992 Reactivity Human Applications: Immunocytochemistry/Immunofluorescence

Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.

Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.

PubMed ID: N/A Reactivity Human Applications: Antibody Microarray

Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.

Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.

PubMed ID: N/A Reactivity Mouse Applications: Antibody Microarray

HDAC6 Regulates Glucocorticoid Receptor Signaling in Serotonin Pathways with Critical Impact on Stress Resilience.

Espallergues, J. et al. (2012) J Neurosci. 32 (13): 4400-4416.

PubMed ID: 22457490 Reactivity Mouse Applications: Immunoprecipitation

ATTO 488

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 804 g/mol

ATTO 488 Datasheet

Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

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