StressMarq/StressXpress®HSP90αELISA试剂盒/SKT-107-96/96孔
商品编号：
SKT-107-96
市场价：
¥12260.00
美元价：
7356.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | HSP90AlphaELISAkit |
| Description | ColorimetricdetectionofHSP90Alpha |
| SpeciesReactivity | Human,Goat |
| Platform | Microplate |
| SampleTypes | Celllysates,Serum,Tissue |
| DetectionMethod | ColorimetricAssay |
| AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
| Utility | ELISAkitusedtoquantitateHSP90alphaconcentrationinsamples. |
| Sensitivity | 0.117ng/ml |
| AssayRange | 0.44-28ng/ml |
| IncubationTime | 30minutes |
| NumberofSamples | 40samplesinduplicate |
| OtherResources | KitBookletLotNo.SH585170,KitBookletLotNo.1008,MSDS |
Properties
| StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||||||||
| ProductType | ELISAKits | ||||||||||||||||||||||||||||||||||||
| AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateat37°Cfor1hour.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatroomtemperature,20-25°Cfor1hour.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm. | ||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | HSP90alphaELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-107) |
BIOLOGicalDescription
| AlternativeNames | HSP86ELISAKit,HSP89AELISAKit,HSP90AELISAKit,HSP90AA1ELISAKit,HSPC1ELISAKit,HSPCAELISAKit,HsoCAL3ELISAKit,HSP90alphaELISAKit |
| ResearchAreas | Cancer,HeatShock |
| ScientificBackground | HSP90isahighlyconservedandessentialstressproteinthatisexpressedinalleukaryoticcells.Fromafunctionalperspective,HSP90participatesinthefolding,assembly,maturation,andstABIlizationofspecificproteinsasanintegralcomponentofachaperonecomplex(1-4).Despiteitslabelofbeingaheat-shockprotein,HSP90isoneofthemosthighlyexpressedproteinsinunstressedcells(1–2%ofcytosolicprotein).Itcarriesoutanumberofhousekeepingfunctions–includingcontrollingtheactivity,turnover,andtraffickingofavarietyofproteins.MostoftheHSP90-regulatedproteinsthathavebeendiscoveredtodateareinvolvedincellsignaling(5-6).ThenumberofproteinsnowknowntointeractwithHSP90isabout100.Targetproteinsincludethekinasesv-Src,Wee1,andc-Raf,transcriptionalregulatorssuchasp53andsteroidreceptors,andthepolymerasesofthehepatitisBvirusandtelomerase(3).WhenboundtoATP,HSP90interactswithco-chaperonesCdc37,p23,andanassortmentofimmunophilin-likeproteins,formingacomplexthatstabilizesandprotectstargetproteinsfromproteasomaldegradation.Inmostcases,HSP90-interactingproteinshavebeenshowntoco-precipitatewithHSP90whencarryingoutimmune-oadsorptionstudies,andtoexistincytosolicheterocomplexeswithit.Inanumberofcases,variationsinHSP90expressionorHSP90mutationhasbeenshowntodegradesignalingfunctionviatheproteinortoimpairaspecificfunctionoftheprotein(suchassteroidbinding,kinaseactivity)invivo.Ansamycinantibiotics,suchasgeldanamycinandrADIcicol,inhibitHSP90function(7).LookingformoreinformationonHSP90?VisitournewHSP90ScientificResourceGuideathttp://www.HSP90.ca. |
| References | 1.ArlanderS.J.H.,etal.(2003)JBiolChem.278:52572-52577. 2.PearlH.,etal.(2001)AdvProteinChem.59:157-186. 3.NeckersL,etal.(2002)TrendsMolMed.8:S55-S61. 4.PrattW.,ToftD.(2003)ExpBiolMed.228:111-133. 5.PrattW.,ToftD.(1997)EndocrRev.18:306-360. 6.PrattW.B.(1998)ProcSocExptlBiolMed.217:420-434. 7.WhitesellL.,etal.(1994)ProcNatlAcadSciUSA.91:8324-8328. |
ProductImages
TypicalStandardCurvefortheHSP90AlphaELISAkit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-107.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.44–28ng/mL.
ProductCitations(3)
Identificationofheatstress-susceptIBLeand-tolerantphenotypesingoatsinsemiaridtropics.
Rout,P.K.,Kaushik,R.,Ramachandran,N.andJindal,S.K.(2017)AnimalProductionScience.
PubMedID:N/AReactivityGoat
OtherCitations
Elevationofserumheat-shockproteinlevelsinamyotrophiclateralsclerosis.
Miyazaki,D.etal.(2016)NeurolSci.37(8):1277-81.
PubMedID:27112486ReactivityHumanApplications:Serum
Highexpressionofheatshockprotein90alphaanditssignificanceinhumanacuteleukemiacells.
Tian,W.L.etal.(2014)Gene.542(2):122-8.
PubMedID:24680776ReactivityHumanApplications:Plasma
品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
