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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/HSP70 Protein/SPR-117A/50-µg
商品详细StressMarq/HSP70 Protein/SPR-117A/50-µg
StressMarq/HSP70 Protein/SPR-117A/50-µg
StressMarq/HSP70 Protein/SPR-117A/50-µg
商品编号: SPR-117A
市场价: ¥6160.00
美元价: 3696.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameHSP70Protein
Description

HumanRecombinantHSP70FullLengthProtein

ApplicationsWB,SDS-PAGE,ATPaseActivityAssay,FunctionalAssay,ELISA
ConcentrationLot/batchspecific.Seeincludeddatasheet.
ConjugatesNotag

Properties

StorageBuffer50mMTris/HClpH7.5,0.3MNaCl,10%glycerol,0.1mMEDTA
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationEndotoxin-free,Multi-StepPurified
Specificity~70kDa
CiteThisProductHumanRecombinantHSP70Protein(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SPR-117)
CertificateofAnalysisThisproducthasbeencertified>90%pureusingSDS-PAGEanalysis.TheproteintestedpositiveforATPaseactivityusingaMalachiteGreenassay.

BIOLOGicalDescription

AlternativeNamesHSP701Protein,HSP702Protein,HSP70.1Protein,HSP72Protein,HSP73Protein,HSPA1Protein,HSPA1AProtein,HSPA1BProtein
ResearchAreasCancer,HeatShock
CellularLocalizationCytoplasm
AccessionNumberM11717
GeneID3303
SwissProtP08107
ScientificBackgroundHSP70genesencodeabundantheat-inducIBLe70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast50%identity(2).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(3).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingABIlitythereforeresidedwithintheC-terminalhalf(4).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(5).AllHSP70s,regardlessoflocation,bindproteins,particularlyunfoldedones.ThemolecularchaperonesoftheHSP70familyrecognizeandbindtonascentpolypeptidechainsaswellaspartiallyfoldedintermediatesofproteinspreventingtheiraggregationandmisfolding.ThebindingofATPtriggersacriticalconformationalchangeleADIngtothereleaseoftheboundsubstrateprotein(6).TheuniversalabilityofHSP70stoundergocyclesofbindingtoandreleasefromhydrophobicstretchesofpartiallyunfoldedproteinsdeterminestheirroleinagreatvarietyofvitalintracellularfunctionssuchasproteinsynthesis,proteinfoldingandoligomerizationandproteintransport.LookingformoreinformationonHSP70?VisitournewHSP70ScientificResourceGuideathttp://www.HSP70.com.
References1.ZhoJ.(1998)Cell.94:471-480.
2.BoorsteinW.R.,ZiegelhofferT.&CraigE.A.(1993)J.Mol.Evol.38(1):1-17.
3.RothmanJ.(1989)Cell.59:591-601.
4.DeLuca-Flahertyetal.(1990)Cell.62:875-887.
5.BorkP.,SanderC.&ValenciaA.(1992)Proc.NatlAcad.Sci.USA.89:7290-7294.
6.FinkA.L.(1999)Physiol.Rev.79:425-449.

ProductImages

<p>SDS-PAGE of endotoxin-free 70kDa native human Hsp70 protein (SPR-117).</p>

SDS-PAGEofendotoxin-free70kDanativehumanHsp70protein(SPR-117).

ProductCitations(1)

OtherCitations

ModulatingEffectofExtracellularHSP70onGenerationofReactiveOxigenSpeciesinPopulationsofPhagocytes.

Troyanova,N.I.etal.(2015)RussianJournalofBioorganicChemistry.41(3):271-279.

PubMedID:26502607Applications:FunctionalAssay

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。