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STRESS MARQ/STRESS XPRESS®HSP70 ELISA试剂盒（工厂）/SKT-124-96/96孔
STRESS MARQ/STRESS XPRESS®HSP70 ELISA试剂盒（工厂）/SKT-124-96/96孔
商品编号：
SKT-124-96
市场价：
¥12880.00
美元价：
7728.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | HSP70ELISAKit(PLANT) |
| Description | ColorimetricdetectionusedtoquantitateHSP70inplants |
| SpeciesReactivity | Human,Alfalfa(Medicagosativa),Flax(Linumusitatissimum),Maize(Zeamays),Plant |
| Platform | Microplate |
| SampleTypes | Celllysates,Plantextract,Plasma,Serum,Tissue |
| DetectionMethod | ColorimetricAssay |
| AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
| Utility | ELISAkitusedtoquantitateHSP70concentrationinsamples. |
| Sensitivity | 0.18ng/ml |
| AssayRange | 1.563-100ng/ml |
| IncubationTime | 30minutes |
| NumberofSamples | 40samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||||||||
| ProductType | ELISAKits | ||||||||||||||||||||||||||||||||||||
| AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardtoappropriatewells.3.CoverplantwithPlateSealerandincubateatroomtemperaturefor1hour.4.WashplatefourtimeswithWashBuffer.5.Add100µLofDetectionAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatroomtemperaturefor1hour.7.Washplatefourtimeswith1XWashBufferasdescribedinstep4.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBufferasdescribedinstep4.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm. | ||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | HSP70ELISAKit(PLANT)(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-124) |
BIOLOGicalDescription
| AlternativeNames | HSP701ELISAKit,HSP702ELISAKit,HSP70.1ELISAKit,HSP72ELISAKit,HSPA1ELISAKit,HSPA1AELISAKit,HSPA1BELISAKit |
| ResearchAreas | Cancer,HeatShock |
| ScientificBackground | HSP70genesencodeabundantheat-inducIBLe70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast50%identity(2).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(3).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingABIlitythereforeresidedwithintheC-terminalhalf(4).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(5).AllHSP70s,regardlessoflocation,bindproteins,particularlyunfoldedones.ThemolecularchaperonesoftheHSP70familyrecognizeandbindtonascentpolypeptidechainsaswellaspartiallyfoldedintermediatesofproteinspreventingtheiraggregationandmisfolding.ThebindingofATPtriggersacriticalconformationalchangeleADIngtothereleaseoftheboundsubstrateprotein(6).TheuniversalabilityofHSP70stoundergocyclesofbindingtoandreleasefromhydrophobicstretchesofpartiallyunfoldedproteinsdeterminestheirroleinagreatvarietyofvitalintracellularfunctionssuchasproteinsynthesis,proteinfoldingandoligomerizationandproteintransport.LookingformoreinformationonHSP70?VisitournewHSP70ScientificResourceGuideathttp://www.HSP70.com. |
| References | 1.BoorsteinW.R.,ZiegelhofferT.&CraigE.A.(1993)J.Mol.Evol.38(1):1-17. 2.RothmanJ.(1989)Cell.59:591-601. 3.DeLuca-Flahertyetal.(1990)Cell.62:875-887. 4.BorkP.,SanderC.&ValenciaA.(1992)Proc.NatlAcad.Sci.USA.89:7290-7294. 5.FinkA.L.(1999)Physiol.Rev.79:425-449. |
ProductImages
TypicalStandardCurvefortheHSP70ELISAKit(PLANT)(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-124.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:1.563–100ng/mL.
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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
