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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/StressXpress®HSP70 ELISA试剂盒/SKT-105-96/96孔
商品详细StressMarq/StressXpress®HSP70 ELISA试剂盒/SKT-105-96/96孔
StressMarq/StressXpress®HSP70 ELISA试剂盒/SKT-105-96/96孔
StressMarq/StressXpress®HSP70 ELISA试剂盒/SKT-105-96/96孔
商品编号: SKT-105-96
市场价: ¥10200.00
美元价: 6120.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameHSP70ELISAKit
Description

ColorimetricdetectionofHSP70

SpeciesReactivityDog,Human,Goat
PlatformMicroplate
SampleTypesCelllysates,Tissue
DetectionMethodColorimetricAssay
AssayTypeSandwichELISA(Enzyme-linkedImmunosorbentAssay)
UtilityELISAkitusedtoquantitateHSP70concentrationinsamples.
Sensitivity0.18ng/ml
AssayRange0.781-50ng/mL
IncubationTime30minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeELISAKits
AssayOverview1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardtoappropriatewells.3.Add50µLofPre-TreatmentBuffertoallsamplewells.4.Add50µLofsampletoappropriatewells.5.CoverplatewithPlateSealerandincubateat37°Cfor2hours.6.Washplatefourtimeswith1XWashBuffer.7.Add100µLofDetectionAntibodyWorkingSolutiontoeachwell.8.CoverplatewithPlateSealerandincubateat37°Cfor2hours.9.Washplatefourtimeswith1XWashBufferasdescribedinstep6.10.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.11.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.12.Washplatefourtimeswith1XWashBufferasdescribedinstep6.13.Add100µLofTMBSubstratetoeachwell.14.Developtheplateinthedarkatroomtemperaturefor30minutes.15.Stopreactionbyadding100µLofStopSolutiontoeachwell.16.Measureabsorbanceonaplatereaderat450nm.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-105AAnti-Hsp70ImmunoassayPlate1Plate
SKC-105B5XHsp70ExtractionReagent1vial/10ml
SKC-105CRecombinantHsp70Standard2vials
SKC-105DStandardandSampleDiluent1vial/50ml
SKC-105E10XWashBufferConcentrate1vial/100ml
SKC-105FAnti-Hsp70BiotinylatedAntibodyConcentrate1vial/150µl
SKC-105GAnti-Hsp70BiotinylatedAntibodyDiluent1vial/13ml
SKC-105HStreptavidin:HRPConcentrate1vial/150µl
SKC-105IStreptavidin:HRPDiluent1vial/13ml
SKC-105JTMBSubstrate1vial/13ml
SKC-105KStopSolution1vial/13ml
SKC-105LPre-treatmentBuffer1vial/13ml
CiteThisProductHSP70ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-105)

BIOLOGicalDescription

AlternativeNamesHSP701ELISAKit,HSP702ELISAKit,HSP70.1ELISAKit,HSP72ELISAKit,HSPA1ELISAKit,HSPA1AELISAKit,HSPA1BELISAKit
ResearchAreasCancer,HeatShock
ScientificBackgroundHSP70genesencodeabundantheat-inducIBLe70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast5O%identity(2).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(3).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingABIlitythereforeresidedwithintheC-terminalhalf(4).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(5).AllHSP70s,regardlessoflocation,bindproteins,particularlyunfoldedones.ThemolecularchaperonesoftheHSP70familyrecognizeandbindtonascentpolypeptidechainsaswellaspartiallyfoldedintermediatesofproteinspreventingtheiraggregationandmisfolding.ThebindingofATPtriggersacriticalconformationalchangeleADIngtothereleaseoftheboundsubstrateprotein(6).TheuniversalabilityofHSP70stoundergocyclesofbindingtoandreleasefromhydrophobicstretchesofpartiallyunfoldedproteinsdeterminestheirroleinagreatvarietyofvitalintracellularfunctionssuchasproteinsynthesis,proteinfoldingandoligomerizationandproteintransport.LookingformoreinformationonHSP70?VisitournewHSP70ScientificResourceGuideathttp://www.HSP70.com.
References1.ZhoJ.(1998)Cell.94:471-480.
2.BoorsteinW.R.,ZiegelhofferT.&CraigE.A.(1993)J.Mol.Evol.38(1):1-17.
3.RothmanJ.(1989)Cell.59:591-601.
4.DeLuca-Flahertyetal.(1990)Cell.62:875-887.
5.BorkP.,SanderC.&ValenciaA.(1992)Proc.NatlAcad.Sci.USA.89:7290-7294.
6.FinkA.L.(1999)Physiol.Rev.79:425-449.
7.SmithD.F.,etal.(1993)Mol.Cell.Biol.13(2):869-876.
8.PrapapanichV.,etal.,(1996)Mol.Cell.Biol.16(11):6200-6207.
9.Fernandez-Funezetal.,(2000)Nature.408(6808):101-106.

ProductImages

<p>Typical Standard Curve for the HSP70 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-105. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.781 – 50 ng/mL.</p>

TypicalStandardCurvefortheHSP70ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-105.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.781–50ng/mL.

ProductCitations(4)

OtherCitations

Urinaryheatshockprotein72asabioMarkerofacutekidneyinjuryindogs.

Bruchim,Y.etal.(2017)VetJ.[Epubaheadofprint].

PubMedID:N/AReactivityDogApplications:Urine

Identificationofheatstress-susceptibleand-tolerantphenotypesingoatsinsemiaridtropics.

Rout,P.K.,Kaushik,R.,Ramachandran,N.andJindal,S.K.(2017)AnimalProductionScience.[Epubaheadofprint].

PubMedID:N/AReactivityGoat

Differentialexpressionpatternofheatshockprotein70geneintissuesandheatstressphenotypesingoatsduringpeakheatstressperiod.

Rout,P.K.,Kaushik,R.andRamachandran,N.(2016)CellStressChaperones.21(4):645-51.

PubMedID:27169748ReactivityGoatApplications:Varioustissues

EnterococcusfaeciumNCIMB10415ModulatesEpithelialIntegrity,HeatShockProtein,andProinflammatoryCytokineResponseinIntestinalCells.

Klingspor,S.etal.(2014)MediatorsInflamm.2015:304149.

PubMedID:25948884ReactivityHumanApplications:Epithelialintestinalcellline

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。