StressMarq/Anti-HSP70 Antibody [7FB]/SMC-230D-A565/100-µg-免疫在线蚂蚁淘旗下平台

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商品详细StressMarq/Anti-HSP70 Antibody [7FB]/SMC-230D-A565/100-µg

StressMarq/Anti-HSP70 Antibody [7FB]/SMC-230D-A565/100-µg

商品编号： SMC-230D-A565

品牌： StressMarq Biosciences

市场价： ¥6540.00

美元价： 3924.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName HSP70Antibody

Description

RatAnti-DrosophilaHSP70MonoclonalIgG2B

SpeciesReactivity FruitFly(Drosophilamelanogaster)

Applications WB,ICC/IF,ELISA

AntibodyDilution WB(1:2000);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Rat

ImmunogenSpecies Drosophila

Immunogen PreparedfromDrosophilatissueculturecellsheatshockedat36.5◦Cfor3hours,andisolatedusingSDSPAGE.

Concentration 1mg/ml

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBuffer	PBSpH7.4,50%glycerol,0.1%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	ProteinGPurified
Clonality	Monoclonal
CloneNumber	7FB
Isotype	IgG2b
Specificity	Detects~70kDa(heat-inducIBLeform).
CiteThisProduct	StressMarqBiosciencesCat#SMC-230,RRID:AB_2699430
CertificateofAnalysis	1µg/mlofSMC-230wassufficientfordetectionofDrosophilaHSP70usinganindirectassaywithrabbitanti-ratIgGandgoatanti-rabbitIgG:HRP.

BIOLOGicalDescription

AlternativeNames	HSP70BbAntibody,HeatShockProtein70BbAntibody,dHSP70Antibody,HSP70bAntibody,HSP70BAntibody,Dm-HSP70Antibody
ResearchAreas	Cancer,HeatShock
AccessionNumber	NP_524927.2
GeneID	48582
SwissProt	Q9BIS2
ScientificBackground	HSP70genesencodeabundantheat-inducible70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast50%identity(2).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(3).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingABIlitythereforeresidedwithintheC-terminalhalf(4).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(5).AllHSP70s,regardlessoflocation,bindproteins,particularlyunfoldedones.ThemolecularchaperonesoftheHSP70familyrecognizeandbindtonascentpolypeptidechainsaswellaspartiallyfoldedintermediatesofproteinspreventingtheiraggregationandmisfolding.ThebindingofATPtriggersacriticalconformationalchangeleADIngtothereleaseoftheboundsubstrateprotein(6).TheuniversalabilityofHSP70stoundergocyclesofbindingtoandreleasefromhydrophobicstretchesofpartiallyunfoldedproteinsdeterminestheirroleinagreatvarietyofvitalintracellularfunctionssuchasproteinsynthesis,proteinfoldingandoligomerizationandproteintransport.FormoreinformationvisitourHSP70ScientificResourceGuideathttp://www.HSP70.com.
References	1.WelchW.J.andSuhanJ.P.(1986)JCellBiol.103:2035-2050. 2.BoorsteinW.R.,ZiegelhofferT.&CraigE.A.(1993)J.Mol.Evol.38(1):1-17. 3.RothmanJ.(1989)Cell59:591-601. 4.DeLuca-Flahertyetal.(1990)Cell62:875-887. 5.BorkP.,SanderC.&ValenciaA.(1992)Proc.Nut1Acad.Sci.USA89:7290-7294. 6.FinkA.L.(1999)Physiol.Rev.79:425-449. 7.GalanA.,etal.(2000)J.Biol.Chem.275:11418-11424. 8.KondoT.,etal.(2000)J.Biol.Chem.275:8872-8879. 9.MisakiT.,etal.(1994)Clin.Exp.Immun.98:234-239. 10.PockleyA.G.,etal.(1998)Immunol.Invest.27:367-377. 11.MoonI.S.,etal.(2001)CerebCortex11(3):238-248. 12.Dresseletal.(2000)J.Immunol.164:2362-2371. 13.VermaA.K.,etal.(2007)FishandShellfishImmunology.22(5):547-555. 14.BanduseelaV.C.,etal.(2009)PhysiolGenomics.39(3):141-159.

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ProductCitations(1)

WesternBlot

TheheatshockresponserestrictsvirusinfectioninDrosophila.

Merkling,S.H.etal.(2015)SciRep.5:12758.

PubMedID:26234525ReactivityDrosophilaApplications:WesternBlot

ATTO565

Overview:

Highfluorescenceyield
Highthermalandphotostability
Goodsolubilityinpolarsolvents
Excellentsolubilityinwater
Verylittleaggregation
Rhodaminedyederivative
MolarMass:611g/mol

ATTO565Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =563nm

λ_em=592nm

ε_max=1.2×10⁵

Φ_f= 0.9

τ_fl =3.4n

Brightness=10

Laser =532nm

Filterset = TRITC

品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

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