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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-HSP70 Antibody [7FB]/SMC-230D/100-µg
商品详细StressMarq/Anti-HSP70 Antibody [7FB]/SMC-230D/100-µg
StressMarq/Anti-HSP70 Antibody [7FB]/SMC-230D/100-µg
StressMarq/Anti-HSP70 Antibody [7FB]/SMC-230D/100-µg
商品编号: SMC-230D
市场价: ¥5600.00
美元价: 3360.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameHSP70Antibody
Description

RatAnti-DrosophilaHSP70MonoclonalIgG2B

SpeciesReactivityFruitFly(Drosophilamelanogaster)
ApplicationsWB,ICC/IF,ELISA
AntibodyDilutionWB(1:2000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRat
ImmunogenSpeciesDrosophila
ImmunogenPreparedfromDrosophilatissueculturecellsheatshockedat36.5◦Cfor3hours,andisolatedusingSDSPAGE.
Concentration1mg/ml
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,0.1%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationProteinGPurified
ClonalityMonoclonal
CloneNumber7FB
IsotypeIgG2b
SpecificityDetects~70kDa(heat-inducIBLeform).
CiteThisProductStressMarqBiosciencesCat#SMC-230,RRID:AB_2699430
CertificateofAnalysis1µg/mlofSMC-230wassufficientfordetectionofDrosophilaHSP70usinganindirectassaywithrabbitanti-ratIgGandgoatanti-rabbitIgG:HRP.

BIOLOGicalDescription

AlternativeNamesHSP70BbAntibody,HeatShockProtein70BbAntibody,dHSP70Antibody,HSP70bAntibody,HSP70BAntibody,Dm-HSP70Antibody
ResearchAreasCancer,HeatShock
AccessionNumberNP_524927.2
GeneID48582
SwissProtQ9BIS2
ScientificBackgroundHSP70genesencodeabundantheat-inducible70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast50%identity(2).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(3).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingABIlitythereforeresidedwithintheC-terminalhalf(4).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(5).AllHSP70s,regardlessoflocation,bindproteins,particularlyunfoldedones.ThemolecularchaperonesoftheHSP70familyrecognizeandbindtonascentpolypeptidechainsaswellaspartiallyfoldedintermediatesofproteinspreventingtheiraggregationandmisfolding.ThebindingofATPtriggersacriticalconformationalchangeleADIngtothereleaseoftheboundsubstrateprotein(6).TheuniversalabilityofHSP70stoundergocyclesofbindingtoandreleasefromhydrophobicstretchesofpartiallyunfoldedproteinsdeterminestheirroleinagreatvarietyofvitalintracellularfunctionssuchasproteinsynthesis,proteinfoldingandoligomerizationandproteintransport.FormoreinformationvisitourHSP70ScientificResourceGuideathttp://www.HSP70.com.
References1.WelchW.J.andSuhanJ.P.(1986)JCellBiol.103:2035-2050.
2.BoorsteinW.R.,ZiegelhofferT.&CraigE.A.(1993)J.Mol.Evol.38(1):1-17.
3.RothmanJ.(1989)Cell59:591-601.
4.DeLuca-Flahertyetal.(1990)Cell62:875-887.
5.BorkP.,SanderC.&ValenciaA.(1992)Proc.Nut1Acad.Sci.USA89:7290-7294.
6.FinkA.L.(1999)Physiol.Rev.79:425-449.
7.GalanA.,etal.(2000)J.Biol.Chem.275:11418-11424.
8.KondoT.,etal.(2000)J.Biol.Chem.275:8872-8879.
9.MisakiT.,etal.(1994)Clin.Exp.Immun.98:234-239.
10.PockleyA.G.,etal.(1998)Immunol.Invest.27:367-377.
11.MoonI.S.,etal.(2001)CerebCortex11(3):238-248.
12.Dresseletal.(2000)J.Immunol.164:2362-2371.
13.VermaA.K.,etal.(2007)FishandShellfishImmunology.22(5):547-555.
14.BanduseelaV.C.,etal.(2009)PhysiolGenomics.39(3):141-159.

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ProductCitations(1)

WesternBlot

TheheatshockresponserestrictsvirusinfectioninDrosophila.

Merkling,S.H.etal.(2015)SciRep.5:12758.

PubMedID:26234525ReactivityDrosophilaApplications:WesternBlot

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。