StressMarq/Anti-HSP70 Antibody [5A5]/SMC-162D-A488/100-µg-免疫在线蚂蚁淘旗下平台

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商品详细StressMarq/Anti-HSP70 Antibody [5A5]/SMC-162D-A488/100-µg

StressMarq/Anti-HSP70 Antibody [5A5]/SMC-162D-A488/100-µg

商品编号： SMC-162D-A488

品牌： StressMarq Biosciences

市场价： ¥6480.00

美元价： 3888.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName HSP70Antibody

Description

MouseAnti-HumanHSP70MonoclonalIgG1

SpeciesReactivity Human,Mouse,Rat,Amphibians,Chicken,Fish,FruitFly(Drosophilamelanogaster),Yeast,Yeast(Saccharomycescerevisiae)

Applications WB,IHC,ICC/IF,IP,AM

AntibodyDilution WB(1:1000),ICC/IF(1:500),IP(1µg);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Mouse

ImmunogenSpecies Human

Immunogen HumanrecombinantHSP70overexpressedinE.coli

Concentration 1mg/ml

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-ProteinComplex
Smallphycobiliprotein
Isolatedfromredalgae
Largestokesshift(195nm)
MolecularWeight:35kDa

PerCPDatasheet

PerCP Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =482nm

λ_em=677nm

ε_max=1.96x10⁶

Laser =488nm

PE/ATTO594

PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.

Overview:

Highfluorescenceyield
HighphotostABIlity
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation

PE/ATTO594Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

OpticalProperties:

λ_ex =535nm

λ_em =627 nm

Laser = 488to561nm

FITC(Fluorescein)

Overview:

Excellentfluorescencequantumyield
Highrateofphotobleaching
Goodsolubilityinwater
Broademissionspectrum
pHdependentspectra
Molecularformula:C₂₀H₁₂O₅
Molarmass:332.3g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =494 nm

λ_em=520 nm

ε_max=7.3×10⁴

Φ_f= 0.92

τ_fl =5.0ns

Brightness= 67.2

Laser = 488 nm

Filterset = FITC

ATTO700

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:575g/mol

ATTO700Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =700 nm

λ_em=719nm

ε_max=1.25×10⁵

Φ_f= 0.25

τ_fl =1.6ns

Brightness= 31.3

Laser =676nm

Filterset =Cy®5.5

ATTO680

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:631g/mol

ATTO680Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =680nm

λ_em=700 nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.7ns

Brightness=37.5

Laser =633 –676nm

Filterset =Cy®5.5

ATTO655

Overview:

Highfluorescenceyield
Highthermalandphotostability
Excellentozoneresistance
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:634g/mol

ATTO655Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =663nm

λ_em=684nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.8ns

Brightness= 37.5

Laser =633 –647nm

Filterset =Cy®5

ATTO633

Overview:

Highfluorescenceyield
Highthermalandphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
StableatpH4–11
Cationicdye,perchloratesalt
MolarMass:652.2g/mol

ATTO633Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =629nm

λ_em=657nm

ε_max=1.3×10⁵

Φ_f= 0.64

τ_fl =3.2ns

Brightness=83.2

Laser =633 nm

Filterset =Cy®5

ATTO594

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:1137g/mol

ATTO594Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

OpticalProperties:

λ_ex =601 nm

λ_em=627nm

ε_max=1.2×10⁵

Φ_f= 0.85

τ_fl =3.5ns

Brightness=102

Laser =594nm

Filterset =TexasRed®

ATTO565

Overview:

Highfluorescenceyield
Highthermalandphotostability
Goodsolubilityinpolarsolvents
Excellentsolubilityinwater
Verylittleaggregation
Rhodaminedyederivative
MolarMass:611g/mol

ATTO565Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =563nm

λ_em=592nm

ε_max=1.2×10⁵

Φ_f= 0.9

τ_fl =3.4n

Brightness=10

Laser =532nm

Filterset = TRITC

ATTO488

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:804g/mol

ATTO488Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =501nm

λ_em=523nm

ε_max=9.0×10⁴

Φ_f= 0.80

τ_fl =4.1ns

Brightness= 72

Laser = 488 nm

Filterset = FITC

ATTO390

Overview:

Highfluorescenceyield
LargeStokes-shift(89nm)
Goodphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
Coumarinderivate,uncharged
Lowmolarmass:343.42g/mol

ATTO390Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

OpticalProperties:

λ_ex =390nm

λ_em=479nm

ε_max=2.4×10⁴

Φ_f= 0.90

τ_fl =5.0ns

Brightness= 21.6

Laser =365or405nm

APC(Allophycocyanin)

Overview:

Highquantumyield
Largephycobiliprotein
6chromophorespermolecule
Isolatedfromredalgae
MolecularWeight:105kDa

APCDatasheet

APC Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =650nm

λ_em=660nm

ε_max=7.0×10⁵

Φ_f= 0.68

Brightness= 476

Laser =594or633 nm

Filterset =Cy®5

Streptavidin

Properties:

Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinity
Molecularweight:53kDa
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

StreptavidinDatasheet

Biotin

Properties:

BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity
Molarmass:244.31g/mol
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

BiotinDatasheet

HRP(HorserADIshperoxidase)

Properties:

Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
Readilycombineswithhydrogenperoxide(H₂O₂)toformHRP-H₂O₂complexwhichcanoxidizevarioushydrogendonors
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
Highturnoverrateenablesrapidgenerationofastrongsignal
44kDaglycoprotein
Extinctioncoefficient:100(403nm)
Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

AP(AlkalinePhosphatase)

Properties:

Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols
Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts
- Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts
Molecularweight:140kDa
Applications:Westernblot,immunohistochemistry,andELISA

APDatasheet

R-PE(R-Phycoerythrin)

Overview:

Broadexcitationspectrum
Highquantumyield
Photostable
Memberofthephycobiliproteinfamily
Isolatedfromredalgae
Excellentsolubilityinwater
MolecularWeight:250kDa

R-PEDatasheet

R-PE Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =565nm

λ_em=575nm

ε_max=2.0×10⁶

Φ_f= 0.84

Brightness=1.68x10³

Laser = 488to561nm

Filterset = TRITC

Properties

StorageBuffer	PBSpH7.2,50%glycerol,0.09%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	ProteinGPurified
Clonality	Monoclonal
CloneNumber	5A5
Isotype	IgG1
Specificity	Detects~70kDa.MaydetectHSP70,HSC70,Grp78andHSP72.
CiteThisProduct	StressMarqBiosciencesCat#SMC-162,RRID:AB_2119819
CertificateofAnalysis	1µg/mlofSMC-162wassufficientfordetectionofHSP70in20µgofheatshockedHeLacelllysatebycolorimetricimmunoblotanalysisusingGoatanti-mouseIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	HSP701Antibody,HSP702Antibody,HSP70.1Antibody,HSP72Antibody,HSPA1Antibody,HSPA1AAntibody,HSPA1BAntibody
ResearchAreas	Cancer,HeatShock
CellularLocalization	Cytoplasm
AccessionNumber	NP_005336.3
GeneID	3303
SwissProt	P08107
ScientificBackground	HSP70genesencodeabundantheat-inducIBLe70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast50%identity(2).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(3).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingabilitythereforeresidedwithintheC-terminalhalf(4).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(5).AllHSP70s,regardlessoflocation,bindproteins,particularlyunfoldedones.ThemolecularchaperonesoftheHSP70familyrecognizeandbindtonascentpolypeptidechainsaswellaspartiallyfoldedintermediatesofproteinspreventingtheiraggregationandmisfolding.ThebindingofATPtriggersacriticalconformationalchangeleadingtothereleaseoftheboundsubstrateprotein(6).TheuniversalabilityofHSP70stoundergocyclesofbindingtoandreleasefromhydrophobicstretchesofpartiallyunfoldedproteinsdeterminestheirroleinagreatvarietyofvitalintracellularfunctionssuchasproteinsynthesis,proteinfoldingandoligomerizationandproteintransport.FormoreinformationvisitourHSP70ScientificResourceGuideathttp://www.HSP70.com.
References	1.BalashovaN.etal.(2005)JBiolChem280:2186-96. 2.BoorsteinW.R.,ZiegelhofferT.&CraigE.A.(1993)J.Mol.Evol.38(1):1-17. 3.RothmanJ.(1989)Cell59:591-601. 4.DeLuca-Flahertyetal.(1990)Cell62:875-887. 5.BorkP.,SanderC.&ValenciaA.(1992)Proc.NatAcad.Sci.USA89:7290-7294. 6.FinkA.L.(1999)Physiol.Rev.79:425-449.

ProductImages

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 5A5 (SMC-162). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (SMC-162) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-HSP70MonoclonalAntibody,Clone5A5(SMC-162).Tissue:CervicalCancercellline(HeLa).Species:Human.Fixation:4%Formaldehydefor15minatRT.PrimaryAntibody:MouseAnti-HSP70MonoclonalAntibody(SMC-162)at1:100for60minatRT.SecondaryAntibody:GoatAnti-MouseATTO488at1:100for60minatRT.Counterstain:DAPI(blue)nuclearstainat1:5000for5minRT.Localization:Nucleus,Cytoplasm.Magnification:40X.

<p>Western Blot analysis of Rat skeletal muscle tissue lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 5A5 (SMC-162). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-162) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.</p>

WesternBlotanalysisofRatskeletalmuscletissuelysateshowingdetectionofHsp70proteinusingMouseAnti-Hsp70MonoclonalAntibody,Clone5A5(SMC-162).Load:15µgprotein.Block:1.5%BSAfor30minutesatRT.PrimaryAntibody:MouseAnti-Hsp70MonoclonalAntibody(SMC-162)at1:1000for2hoursatRT.SecondaryAntibody:SheepAnti-MouseIgG:HRPfor1houratRT.

ProductCitations(2)

OtherCitations

BioMarkerAnalysiswithGratingCoupledSurfacePlasmonCoupledFluorescence.

Mendoza,A.,Dias,J.A.,Zeltner,T.andLawrence,D.A.(2014)JAdvBio&Biotech.1(1):1-22.

PubMedID:N/AReactivityHumanApplications:AntibodyMicroarray

BiomarkerAnalysiswithGratingCoupledSurfacePlasmonCoupledFluorescence.

Mendoza,A.,Dias,J.A.,Zeltner,T.andLawrence,D.A.(2014)JAdvBio&Biotech.1(1):1-22.

PubMedID:N/AReactivityMouseApplications:AntibodyMicroarray

ATTO488

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:804g/mol

ATTO488Datasheet

OpticalProperties:

λ_ex =501nm

λ_em=523nm

ε_max=9.0×10⁴

Φ_f= 0.80

τ_fl =4.1ns

Brightness= 72

Laser = 488 nm

Filterset = FITC

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StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

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