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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-HSP70 (Fish) Antibody/SPC-314B/200-µg
商品详细StressMarq/Anti-HSP70 (Fish) Antibody/SPC-314B/200-µg
StressMarq/Anti-HSP70 (Fish) Antibody/SPC-314B/200-µg
StressMarq/Anti-HSP70 (Fish) Antibody/SPC-314B/200-µg
商品编号: SPC-314B
市场价: ¥7180.00
美元价: 4308.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameHSP70(Fish)Antibody
Description

RabbitAnti-SalmonHSP70(Fish)Polyclonal

SpeciesReactivityBrookTrout(Salvelinusfontinalis),Fish,RainbowTrout(Oncorhynchusmykiss),Salmon
ApplicationsWB,IHC,IP
AntibodyDilutionWB(1:1000),IHC(1:100),IP(5µg);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesSalmon
ImmunogenSyntheticpeptideconjugatedtoKLH.PeptidesequencespecifictoC-terminalpartofsalmonidHSP70.ThetargetisnotpresentinanyotherHSP70proteins.
ConjugatesUnconjugated

Properties

StorageBufferLyophilizedrabbitAntiserum.Forreconstitutionadd200µlofsterilewater.
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationProteinApurified
ClonalityPolyclonal
SpecificityDetects~70kDa.ItdoesnotcrossreactwithHSC70.ItdoesnotdetectHSP70inotherspecies.
CiteThisProductStressMarqBiosciencesCat#SPC-314,RRID:AB_1501441
CertificateofAnalysis1µg/mlofSPC-314wassufficientfordetectionofHSP70in20µgofheatshocked(25°C)salmoncytoplasmicfractionlysatebycolorimetricimmunoblotanalysisusingGoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesHSP701Antibody,HSP702Antibody,HSP70.1Antibody,HSP72Antibody,HSPA1Antibody,HSPA1AAntibody,HSPA1BAntibody
ResearchAreasCancer,HeatShock
CellularLocalizationCytoplasm
AccessionNumberNP_001135156.1
GeneID100196655
SwissProtB5DG30
ScientificBackgroundHSP70genesencodeabundantheat-inducible70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast5O%identity(1).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(2).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingabilitythereforeresidedwithintheC-terminalhalf(3).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(4).Whencellsaresubjectedtometabolicstress(e.g.,heatshock)amemberoftheHSP70family,HSP70(HSP72),isexpressed;HSP70ishighlyrelatedtoHSC70(>90%sequenceidentity).ConstitutivelyexpressedHSC70rapidlyformsastablecomplexwiththehighlyinducIBLeHSP70incellsfollowingheatshock.TheinteractionofHSC70withHSP70isregulatedbyATP.Thesetwoheatshockproteinsmovetogetherinthecellexperiencingstress.FurThermore,researchonHSC70hasimplicatesitwitharoleinfacilitatingtherecoveryofcentrosomalstructureandfunctionafterheatshock(5).LookingformoreinformationonHSP70?VisitournewHSP70ScientificResourceGuideathttp://www.HSP70.com.
References1.BoorsteinW.R.,ZiegelhofferT.,andCraigE.A.(1993)J.Mol.Evol.38(1):1-17.
2.RothmanJ.(1989)Cell.59:591-601.
3.DeLuca-Flahertyetal.(1990)Cell.62:875-887.
4.BorkP.,SanderC.,andValenciaA.(1992)Proc.NatlAcad.Sci.USA.89:7290-7294.
5.BrownC.L.etal.(1996)J.Biol.Chem.271(2):833-840.

ProductImages

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ProductCitations(1)

WesternBlot

DevelopmentofRTgutGCDevelopmentofRTgutGCasaToolforFishFeedDevelopment.

Pumputis,P.G.(2017)UniverisityofWaterloo.Dissertation.

PubMedID:N/AReactivityRainbowTroutApplications:WesternBlot

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。