StressMarq/Anti-HSP70 (Fish) Antibody/SPC-313D/100-µl-免疫在线蚂蚁淘旗下平台

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商品详细StressMarq/Anti-HSP70 (Fish) Antibody/SPC-313D/100-µl

StressMarq/Anti-HSP70 (Fish) Antibody/SPC-313D/100-µl

商品编号： SPC-313D

品牌： StressMarq Biosciences

市场价： ¥6160.00

美元价： 3696.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName HSP70(Fish)Antibody

Description

RabbitAnti-SalmonHSP70(Fish)Polyclonal

SpeciesReactivity BrookTrout(Salvelinusfontinalis),Fish,RainbowTrout(Oncorhynchusmykiss),Salmon,Three-spinedstickleback(Gasterosteusaculeatus)

Applications WB

AntibodyDilution WB(1:5000);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Rabbit

ImmunogenSpecies Salmon

Immunogen SyntheticpeptideconjugatedtoKLH.PeptidesequencespecifictoC-terminalpartofsalmonidHSP70.ThetargetisnotpresentinanyotherHSP70proteins.

Conjugates

Unconjugated

Properties

StorageBuffer	LyophilizedrabbitAntiserum.Forreconstitutionadd100µlofsterilewater.
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	Rabbitantiserum
Clonality	Polyclonal
Specificity	Detects~70kDa.ItdoesnotcrossreactwithHSC70.ItdoesnotdetectHSP70inotherspecies.
CiteThisProduct	StressMarqBiosciencesCat#SPC-313,RRID:AB_1501439
CertificateofAnalysis	0.2µl/mlofSPC-313wassufficientfordetectionofHSP70in20µgofheatshocked(25°C)rainbowtroutcytoplasmicfractionlysatebycolorimetricimmunoblotanalysisusingGoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	HSP701Antibody,HSP702Antibody,HSP70.1Antibody,HSP72Antibody,HSPA1Antibody,HSPA1AAntibody,HSPA1BAntibody
ResearchAreas	Cancer,HeatShock
CellularLocalization	Cytoplasm
AccessionNumber	NP_001135156.1
GeneID	100196655
SwissProt	B5DG30
ScientificBackground	HSP70genesencodeabundantheat-inducible70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast5O%identity(1).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(2).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingabilitythereforeresidedwithintheC-terminalhalf(3).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(4).Whencellsaresubjectedtometabolicstress(e.g.,heatshock)amemberoftheHSP70family,HSP70(HSP72),isexpressed;HSP70ishighlyrelatedtoHSC70(>90%sequenceidentity).ConstitutivelyexpressedHSC70rapidlyformsastablecomplexwiththehighlyinducIBLeHSP70incellsfollowingheatshock.TheinteractionofHSC70withHSP70isregulatedbyATP.Thesetwoheatshockproteinsmovetogetherinthecellexperiencingstress.FurThermore,researchonHSC70hasimplicatesitwitharoleinfacilitatingtherecoveryofcentrosomalstructureandfunctionafterheatshock(5).LookingformoreinformationonHSP70?VisitournewHSP70ScientificResourceGuideathttp://www.HSP70.com.
References	1.BoorsteinW.R.,ZiegelhofferT.,andCraigE.A.(1993)J.Mol.Evol.38(1):1-17. 2.RothmanJ.(1989)Cell.59:591-601. 3.DeLuca-Flahertyetal.(1990)Cell.62:875-887. 4.BorkP.,SanderC.,andValenciaA.(1992)Proc.NatlAcad.Sci.USA.89:7290-7294. 5.BrownC.L.etal.(1996)J.Biol.Chem.271(2):833-840.

ProductImages

<p>Western blot analysis of Rainbow trout Tissue lysates showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody (SPC-313). Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (SPC-313) at 1:5000.</p>

WesternblotanalysisofRainbowtroutTissuelysatesshowingdetectionofHSP70proteinusingRabbitAnti-HSP70PolyclonalAntibody(SPC-313).PrimaryAntibody:RabbitAnti-HSP70PolyclonalAntibody(SPC-313)at1:5000.

ProductCitations(4)

WesternBlot

Coldacclimationincreaseslevelsofsomeheatshockproteinandsirtuinisoformsinthreespinestickleback.

Teigen,L.E.,Orczewska,J.I.,McLaughlin,J.(2015)CompBiochemPhysiolAMolIntegrPhysiol.188:139-47.

PubMedID:26123780ReactivityGasterosteusaculeatus(Three-spinedstickleback)Applications:WesternBlot

Temporalchangesinstressandtissue-specificmetabolicresponsestomunicipalwastewatereffluentexposureinrainbowtrout.

Ings,J.S.,Oakes,K.D.,Vijayan,M.M.,andServos,M.R.(2012)CompBiochemPhysiolCToxicolPharmacol.156(2):67-74.

PubMedID:22579662ReactivityTroutApplications:WesternBlot

Hepatictranscriptomicsandproteinexpressioninrainbowtroutexposedtomunicipalwastewatereffluent.

Ings,J.S.,Servos,M.R.andVijayan,M.M.(2011)EnvironSciTechnol.45(6):2368-2376.

PubMedID:21322548ReactivityTroutApplications:WesternBlot

OtherCitations

PlasmaexosomesareenrichedinHsp70andmodulatedbystressandcortisolinrainbowtrout.

Faught,E.,Henrickson,L.andVijayan,M.M.(2016)JEndocrinol.232(2):237-246.

PubMedID:27872197ReactivityRainbowTrout

品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

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