StressMarq/StressXpress®HSP65 ELISA试剂盒/SKT-113-96/96孔
商品编号：
SKT-113-96
市场价：
¥11220.00
美元价：
6732.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | HSP65ELISAKit |
| Description | ColorimetricdetectionofHSP65 |
| SpeciesReactivity | MycobacteriumBovis,MycobacteriumTuberculosum |
| Platform | Microplate |
| SampleTypes | Celllysates,Serum,Tissue |
| DetectionMethod | ColorimetricAssay |
| AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
| Utility | ELISAkitusedtoquantitateM.bovis/M.tuberculosumHSP65concentrationinhumansamples |
| Sensitivity | 0.06ng/ml |
| AssayRange | 0.344-22ng/ml |
| IncubationTime | 30minutes |
| NumberofSamples | 40samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||||||||
| ProductType | ELISAKits | ||||||||||||||||||||||||||||||||||||
| AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateatroomtemperature(20-25°C)for1hour.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatroomtemperaturefor1hour.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm. | ||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | HSP65ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-113) |
BIOLOGicalDescription
| AlternativeNames | 60kDachaperonin2ELISAKit,CellwallproteinAELISAKit,groELProtein2ELISAKit,GroEL2ELISAKit,M.TuberculosiscellwallproteinAELISAKit,M.TuberculosisHSP65ELISAKit |
| ResearchAreas | Cancer,HeatShock |
| ScientificBackground | HSP65isamemberoftheHSP60familyofheatshockproteins(2,3).HSP60saremitochondrialchaperoninsthataretypicallyheldresponsIBLeforthetransportationandrefoldingofproteinsfromthecytoplasmintothemitochondrialmatrix.Inadditiontoitsroleasaheatshockprotein,HSP60functionsasachaperonintoassistinfoldinglinearaminoacidchainsintotheirrespectivethree-dimensionalstructure.HSP60sareaubiquitousclassofHSPsthatspecificallypromotethefoldingandassemblyofcellularpolypeptidesinanATP-dependentmanner(1).Specifically,sequencecomparisonofHSP65fromdifferentmycobacteriumstrainsshowedthattheproteinsequenceofM.bovisBCGisidenticaltothatofM.tuberculosis,andverysimilartothatofM.leprae,thepathogensthatcausetuberculosisandtuberculoidleprosy,respectively(2,4).MycobacteriumbovisBCGHSP65wasidentifiedastheimmunodominantantigenduringmycobacterialdiseasesandvaccination.Itisalsobelievedtobetheantigenthatinducesautoimmunedisease,suchasadjuvantarthritisinrats(5,6). |
| References | 1.KollH.,etal.(1992)Cell.68:1163-1175. 2.TholeJ.E.R.,etal.(1985)Infect.Immuno.50:800-806. 3.TholeJ.E.R.,etal.,(1987)Infect.Immuno.55:1466-1475. 4.ShinnickT.M.SweetserD.,TholeJ.,vanEmBDenJ.andYoungR.A.(1987)Infect.Immuno.55:1932-1935. 5.VanEdenW.,etal.(1988)Nature331:171-178. 6.CobelensP.M.,etal.(2002)Rheumatology41:775-779. |
ProductImages
TypicalStandardCurvefortheHSP65ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-113.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.344–22ng/mL.
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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
