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StressMarq/StressXpress® HSP60 ELISA Kit/SKT-110-96/96-well
StressMarq/StressXpress® HSP60 ELISA Kit/SKT-110-96/96-well
商品编号：
SKT-110-96
市场价：
¥10280.00
美元价：
6168.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | HSP60ELISAKit |
| Description | ColorimetricdetectionofHSP60 |
| SpeciesReactivity | Human |
| Platform | Microplate |
| SampleTypes | Celllysates,Serum,Tissue |
| DetectionMethod | ColorimetricAssay |
| AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
| Utility | ELISAkitusedtoquantitateHSP60concentrationinsamples. |
| Sensitivity | 1.73ng/ml |
| AssayRange | 11-700ng/ml |
| IncubationTime | 30minutes |
| NumberofSamples | 40samplesinduplicate |
| OtherResources | KitBookletLotNo.BH585599,KitBookletLotNo.TH585578,KitBookletLotNo.160427,MSDS |
Properties
| StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||||||||
| ProductType | ELISAKits | ||||||||||||||||||||||||||||||||||||
| AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateat37°Cfor1hour.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateat37°Cfor1hour.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm. | ||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | HSP60ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-110) |
BIOLOGicalDescription
| AlternativeNames | CPN60ELISAKit,GROELELISAKit,HLD4ELISAKit,HSP60ELISAKit,HSP65ELISAKit,HSPD1ELISAKit,HuCHA60ELISAKit,SPG13ELISAKit |
| ResearchAreas | Cancer,HeatShock |
| ScientificBackground | Inbothprokaryoticandeukaryoticcells,themisfoldingandaggregationofproteinsduringbiogenesisandunderconditionsofcellularstressarepreventedbymolecularchaperones.MembersoftheHSP60familyofheatshockproteinsaresomeofthebestcharacterizedchaperones.HSP60,alsoknownasCpn60orGroEl,isanabundantproteinsynthesizedconstitutivelyinthecellthatisinducedtoahigherconcentrationafterbriefcellshock.Itispresentinmanyspeciesandexhibitsaremarkablesequencehomologyamongvariouscounterpartsinbacteria,plants,andmammalswithmorethanhalfoftheresiduesidenticalbetweenbacterialandmammalianHSP60(1-3).WhereasmammalianHSP60islocalizedwithinthemitochondria,plantHSP60,orotherwiseknownasRubisco-bindingprotein,islocatedinplantchloroplasts.IthasbeenindicatedthattheseproteinscarryoutaveryimportantbiologicalfunctionduetothefactthatHSP60ispresentinsomanydifferentspecies.ThecommoncharacteristicsoftheHSP60sfromthedivergentspeciesarei)highabundance,ii)inductionwithenvironmentalstresssuchasheatshock,iii)homo-oligomericstructuresofeither7or14subunitswhichreversIBLydissociateinthepresenceofMg2+andATP,iv)ATPaseactivityandv)aroleinfoldingandassemblyofoligomericproteinstructures(4).Thesesimilaritiesaresupportedbyrecentstudieswherethesingle-ringhumanmitochondrialhomolog,HSP60withitsco-chaperonin,HSP10wereexpressedinaE.colistrain,engineeredsothatthegroEoperonisunderstrictregulatorycontrol.ThisstudyhasdemonstratedthatexpressionofHSP60-HSP10wasabletocarryoutallessentialinvivofunctionsofGroELanditsco-chaperonin,GroES(5).AnotherimportantfunctionofHSP60andHSP10istheirprotectivefunctionsagainstinfectionandcellularstress.HSP60hashoweverbeenlinkedtoanumberofautoimmunediseases,aswellasAlzheimers,coronaryarterydiseases,MS,anddiabetes(6-9). |
| References | 1.HartlF.U.(1996)Nature.381:571-579. 2.BukauB.andHorwich,A.L.(1998)Cell.92:351-366. 3.HartlF.U.andHayer-Hartl,M.(2002)Science.295:1852-1858. 4.JindalS.,etal.(1989)MolecularandCellularBiology.9:2279-2283. 5.LaVerda,D.,etal(1999)InfectDis.Obstet.Gynecol.7:64-71. 6.ItohH.,etal.(2002)Eur.J.Biochem.269:5931-5938. 7.GuptaS.andKnowltonA.A.J.CellMol.Med.9:51-58. 8.DeocarisC.C.,etal.(2006)CellStressChaperones.11:116-128. 9.LaiH.C.,etal.(2007)Am.J.Physiol.Endocrinol.Metab.292:E292-E297. |
ProductImages
TypicalStandardCurvefortheHSP60ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-110.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:7–450ng/mL.
TypicalStandardCurvefortheHSP60ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-110.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:11–700ng/ml.
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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
