StressMarq/Anti-HSP27 Antibody (Ser15)/SPC-1260D-HRP/100-µl-免疫在线蚂蚁淘旗下平台

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商品详细StressMarq/Anti-HSP27 Antibody (Ser15)/SPC-1260D-HRP/100-µl

StressMarq/Anti-HSP27 Antibody (Ser15)/SPC-1260D-HRP/100-µl

商品编号： SPC-1260D-HRP

品牌： StressMarq Biosciences

市场价： ¥5800.00

美元价： 3480.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName HSP27Antibody(Ser15)

Description

RabbitAnti-HumanHSP27(Ser15)Polyclonal

SpeciesReactivity Human

Applications WB,IHC

AntibodyDilution WB(1:1000),IHC(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Rabbit

ImmunogenSpecies Human

Immunogen SynthesizedunphosphorylatedpeptidederivedfromhumanHSP27aroundthephosphorylationsiteofserine15(G-P-SP-W-D).

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBuffer	PBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	AffinityPurified
Clonality	Polyclonal
Isotype	IgG
Specificity	DetectsendogenouslevelsoftotalHSP27protein.
CiteThisProduct	StressMarqBiosciencesCat#SPC-1260,RRID:AB_2712485
CertificateofAnalysis	A1:1000dilutionofSPC-1260wassufficientfordetectionofHsp27in10µgofHeLacelllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	28kDaheatshockproteinAntibody,CMT2FAntibody,HSP25Antibody,HSP27Antibody,HSP28Antibody,HSPB1Antibody,SRP27Antibody
ResearchAreas	Cancer,HeatShock,Atherosclerosis,CardiovascularSystem,ChaperoneProteins,Chaperones,Heart,ProteinTrafficking,Trafficking
CellularLocalization	Cytoplasm,Nucleus
AccessionNumber	NP_001531.1
GeneID	3315
SwissProt	P04792
ScientificBackground	HSP27sbelongtoanabundantandubiquitousfamilyofsmallheatshockproteins(sHSP).ItisanimportantHSPfoundinbothnormalhumancellsandcancercells.ThebasicstructureofmostsHSPsisahomologousandhighlyconservedaminoacidsequence,withanα-crystallindomainattheC-terminusandtheWD/EPFdomainatthelessconservedN-terminus.ThisN-terminusisessentialforthedevelopmentofhighmolecularoligomers(1,2).HSP27-oligomersconsistofstabledimersformedbyasmanyas8-40HSP27proteinmonomers(3).Theoligomerizationstatusisconnectedwiththechaperoneactivity:aggregatesoflargeoligomershavehighchaperoneactivity,whereasdimershavenochaperoneactivity(4).HSP27islocalizedtothecytoplasmofunstressedcellsbutcanredistributetothenucleusinresponsetostress,whereitmayfunctiontostABIlizeDNAand/orthenuclearmembrane.Otherfunctionsincludechaperoneactivity(asmentionedabove),Thermotoleranceinvivo,inhibitionofapoptosis,andsignaltransduction.Specifically,invitro,itactsasanATP-independentchaperonebyinhibitingproteinaggregationandbystabilizingpartiallydenaturedproteins,whichensuresrefoldingoftheHSP70complex.HSP27isalsoinvolvedintheapoptoticsignalingpathwaybecauseitinterfereswiththeactivationofcytochromec/Apaf-1/dATPcomplex,therebyinhibitingtheactivationofprocaspase-9.ItisalsohypothesizedthatHSP27mayservesomeroleincross-bridgeformationbetweenactinandmyosin(5).Andfinally,HSP27isalsothoughttobeinvolvedintheprocessofcelldifferentiation.Theup-regulationofHSP27correlateswiththerateofphosphorylationandwithanincreaseoflargeoligomers.ItispossIBLethatHSP27mayplayacrucialroleinterminationofgrowth(6).LookingformoreinformationonHSP27?VisitournewHSP27ScientificResourceGuideathttp://www.HSP27.com.
References	1.KimK.K.,KimR.,andKim,S.(1998)Nature394(6693):595-599. 2.VanMontfortR.,SlingsbyC.,andVierlingE.(2001)AddvProteinChem.59:105-56. 3.EhrnspergerM.,GraberS.,GaestelM.andBuchnerJ.(1997)EMBOJ.16:221-229. 4.CioccaD.R.,OesterreichS.,ChamnessG.C.,McGuireW.L.,andFuguaS.A.(1993)JNatlCancerInst.85(19):1558-70. 5.SartoC.,BinnzP.A.,andMocarelliP.(2000)Electrophoresis.21(6):1218-26. 6.ArrigoA.P.(2005)JCellBiochem.94(2):241-6.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1260). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1260) at 1:1000. The image on the right is treated with the synthesized peptide.</p>

ImmunohistochemistryanalysisusingRabbitAnti-Hsp27PolyclonalAntibody(SPC-1260).Tissue:BreastCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-Hsp27PolyclonalAntibody(SPC-1260)at1:1000.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Western blot analysis of Human HeLa cell lysates showing detection of ~27kDa Hsp27 protein using Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1260). Lane 1 and Lane 2: Human HeLa, Hsp27 Antibody (Ser15). Lane 3 and Lane 4: Human HeLa, Hsp27 Antibody (pSer15). Primary Antibody: Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1260) at 1:1000. Predicted/Observed Size: ~27kDa.</p>

WesternblotanalysisofHumanHeLacelllysatesshowingdetectionof~27kDaHsp27proteinusingRabbitAnti-Hsp27PolyclonalAntibody(SPC-1260).Lane1andLane2:HumanHeLa,Hsp27Antibody(Ser15).Lane3andLane4:HumanHeLa,Hsp27Antibody(pSer15).PrimaryAntibody:RabbitAnti-Hsp27PolyclonalAntibody(SPC-1260)at1:1000.Predicted/ObservedSize:~27kDa.

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HRP(HorserADIshperoxidase)

Properties:

Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
Readilycombineswithhydrogenperoxide(H₂O₂)toformHRP-H₂O₂complexwhichcanoxidizevarioushydrogendonors
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
Highturnoverrateenablesrapidgenerationofastrongsignal
44kDaglycoprotein
Extinctioncoefficient:100(403nm)
Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

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