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当前位置： 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-HSP27 Antibody (pSer15)/SPC-1263D-A488/100-µl
商品详细StressMarq/Anti-HSP27 Antibody (pSer15)/SPC-1263D-A488/100-µl
StressMarq/Anti-HSP27 Antibody (pSer15)/SPC-1263D-A488/100-µl
StressMarq/Anti-HSP27 Antibody (pSer15)/SPC-1263D-A488/100-µl
商品编号： SPC-1263D-A488
市场价： ¥6040.00
美元价： 3624.00
产地： 美国(厂家直采)
公司：
产品分类： 酸碱缓冲液
公司分类： acid_base_buffer_solution
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

ProductNameHSP27Antibody(pSer15)
Description

RabbitAnti-HumanHSP27(pSer15)Polyclonal

SpeciesReactivityHuman,Mouse,Rat
ApplicationsWB,IHC,ICC/IF
AntibodyDilutionWB(1:1000),IHC(1:100),ICC/IF(1:200);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenSynthesizedphosphopeptidederivedfromhumanHSP27aroundthephosphorylationsiteofserine15(G-P-SP-W-D).
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityPolyclonal
IsotypeIgG
SpecificityDetectsendogenouslevelsofHSP27onlywhenphosphorylatedatserine15.
CiteThisProductStressMarqBiosciencesCat#SPC-1263,RRID:AB_2712539
CertificateofAnalysisA1:1000dilutionofSPC-1263wassufficientfordetectionofHsp27in10µgofHeLacelllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames28kDaheatshockproteinAntibody,CMT2FAntibody,HSP25Antibody,HSP27Antibody,HSP28Antibody,HSPB1Antibody,SRP27Antibody
ResearchAreasCancer,HeatShock,Atherosclerosis,CardiovascularSystem,ChaperoneProteins,Chaperones,Heart,ProteinTrafficking,Trafficking
CellularLocalizationCytoplasm,Nucleus
AccessionNumberNP_001531.1
GeneID3315
SwissProtP04792
ScientificBackgroundHSP27sbelongtoanabundantandubiquitousfamilyofsmallheatshockproteins(sHSP).ItisanimportantHSPfoundinbothnormalhumancellsandcancercells.ThebasicstructureofmostsHSPsisahomologousandhighlyconservedaminoacidsequence,withanα-crystallindomainattheC-terminusandtheWD/EPFdomainatthelessconservedN-terminus.ThisN-terminusisessentialforthedevelopmentofhighmolecularoligomers(1,2).HSP27-oligomersconsistofstabledimersformedbyasmanyas8-40HSP27proteinmonomers(3).Theoligomerizationstatusisconnectedwiththechaperoneactivity:aggregatesoflargeoligomershavehighchaperoneactivity,whereasdimershavenochaperoneactivity(4).HSP27islocalizedtothecytoplasmofunstressedcellsbutcanredistributetothenucleusinresponsetostress,whereitmayfunctiontostABIlizeDNAand/orthenuclearmembrane.Otherfunctionsincludechaperoneactivity(asmentionedabove),Thermotoleranceinvivo,inhibitionofapoptosis,andsignaltransduction.Specifically,invitro,itactsasanATP-independentchaperonebyinhibitingproteinaggregationandbystabilizingpartiallydenaturedproteins,whichensuresrefoldingoftheHSP70complex.HSP27isalsoinvolvedintheapoptoticsignalingpathwaybecauseitinterfereswiththeactivationofcytochromec/Apaf-1/dATPcomplex,therebyinhibitingtheactivationofprocaspase-9.ItisalsohypothesizedthatHSP27mayservesomeroleincross-bridgeformationbetweenactinandmyosin(5).Andfinally,HSP27isalsothoughttobeinvolvedintheprocessofcelldifferentiation.Theup-regulationofHSP27correlateswiththerateofphosphorylationandwithanincreaseoflargeoligomers.ItispossIBLethatHSP27mayplayacrucialroleinterminationofgrowth(6).LookingformoreinformationonHSP27?VisitournewHSP27ScientificResourceGuideathttp://www.HSP27.com.
References1.KimK.K.,KimR.,andKim,S.(1998)Nature394(6693):595-599.
2.VanMontfortR.,SlingsbyC.,andVierlingE.(2001)AddvProteinChem.59:105-56.
3.EhrnspergerM.,GraberS.,GaestelM.andBuchnerJ.(1997)EMBOJ.16:221-229.
4.CioccaD.R.,OesterreichS.,ChamnessG.C.,McGuireW.L.,andFuguaS.A.(1993)JNatlCancerInst.85(19):1558-70.
5.SartoC.,BinnzP.A.,andMocarelliP.(2000)Electrophoresis.21(6):1218-26.
6.ArrigoA.P.(2005)JCellBiochem.94(2):241-6.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1263). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1263) at 1:1000. The image on the right is treated with the synthesized peptide.</p>

ImmunohistochemistryanalysisusingRabbitAnti-Hsp27PolyclonalAntibody(SPC-1263).Tissue:BreastCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-Hsp27PolyclonalAntibody(SPC-1263)at1:1000.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Western blot analysis of Human HeLa cell lysates showing detection of ~27kDa Hsp27 protein using Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1263). Lane 1 and Lane 2: Human HeLa, Hsp27 Antibody (Ser15). Lane 3 and Lane 4: Human HeLa, Hsp27 Antibody (pSer15). Primary Antibody: Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1263) at 1:1000. Predicted/Observed Size: ~27kDa.</p>

WesternblotanalysisofHumanHeLacelllysatesshowingdetectionof~27kDaHsp27proteinusingRabbitAnti-Hsp27PolyclonalAntibody(SPC-1263).Lane1andLane2:HumanHeLa,Hsp27Antibody(Ser15).Lane3andLane4:HumanHeLa,Hsp27Antibody(pSer15).PrimaryAntibody:RabbitAnti-Hsp27PolyclonalAntibody(SPC-1263)at1:1000.Predicted/ObservedSize:~27kDa.

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 ATTO488
Overview:
  • Highfluorescenceyield
  • Highphotostability
  • Veryhydrophilic
  • Excellentsolubilityinwater
  • Verylittleaggregation
  • Newdyewithnetchargeof-1
  • MolarMass:804g/mol 

ATTO488Datasheet

  ATTO 488 Fluorophore Excitation and Emission SpectraOpticalProperties:

λex =501nm

λem=523nm

εmax=9.0×104

Φf= 0.80

τfl =4.1ns

Brightness= 72

Laser = 488 nm

Filterset = FITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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CLIA套件 兴奋剂 敌手 抑制剂 蛋白质 活化剂 抗氧化剂 氧化还原探针 多克隆
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