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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-HSP101/ClpB NT (Plant) Antibody/SPC-304B/200-µl
商品详细StressMarq/Anti-HSP101/ClpB NT (Plant) Antibody/SPC-304B/200-µl
StressMarq/Anti-HSP101/ClpB NT (Plant) Antibody/SPC-304B/200-µl
StressMarq/Anti-HSP101/ClpB NT (Plant) Antibody/SPC-304B/200-µl
商品编号: SPC-304B
市场价: ¥6160.00
美元价: 3696.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameHSP101/ClpBNT(Plant)Antibody
Description

RabbitAnti-PlantHSP101/ClpBNT(Plant)Polyclonal

SpeciesReactivityArABIdopsisthaliana,Monocot(Agavetequilana),Plant
ApplicationsWB
AntibodyDilutionWB(1:1000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesPlant
ImmunogenRecombinantproteinArabidopsisthalianaHSP101/ClpBN-terminal
ConjugatesUnconjugated

Properties

StorageBufferLyophilizedrabbitAntiserum.Forreconstitutionadd200µlofsterilewater.
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationRabbitantiserum
ClonalityPolyclonal
SpecificityDetectstheN-terminaldomainofHSP101~101kDa.
CiteThisProductStressMarqBiosciencesCat#SPC-304,RRID:AB_1501431
CertificateofAnalysis1µl/mlofSPC-304wassufficientfordetectionofHSP101in2µgofArabidopsisthalianaWTlysatestressedat38°Cfor1.5hoursbycolorimetricimmunoblotanalysisusingGoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesheatshockprotein1010Antibody,HOT1Antibody,HSP101Antibody,HSPSAntibody
ResearchAreasCancer,HeatShock,CellSignaling,ChaperoneProteins,ProteinTrafficking
CellularLocalizationCytoplasm
AccessionNumberNP_565083.1
GeneID843771
SwissProtP42730
ScientificBackgroundHSPs/chaperonesareknowntobeexpressedinplantsnotonlywhentheyexperiencehightemperaturesstress,butalsoinresponsetoawiderangeofotherenvironmentalstressessuchaswaterstress,saltstress,coldstressandoxidativestress(1,2).HSP100isonesuchmajorclassthatactsasachaperone.HSP101/ClpBisamemberofthisHSP100family.Theseproteinshelpdissociateproteinaggregatesformedduringheatstresstoallowthemtoberefoldedbyotherchaperones.Inspiteofexpressionduringheatstress,membersofHSP100proteinfamilyarealsoexpressedduringseeddevelopment.HSP101proteinislocalizedinacytoplasm.
References1.VierlingE.(1991)AnnuRev.PlantPhysiol.PlantMol.Biol.42:579-620.
2.WatersW.,etal.(2004)TrendsPlantSci.9:244-252.

ProductImages

<p>Western blot analysis of Arabidopsis Arabidopsis thalina tissue showing detection of HSP101 protein using Rabbit Anti-HSP101 Polyclonal Antibody (SPC-304). Load: 2 µg. Primary Antibody: Rabbit Anti-HSP101 Polyclonal Antibody (SPC-304) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP-conjugated Goat Anti-Rabbit at 1:10000. From left to right: Arabidopsis thaliana WT stressed at 38°C for 1.5 hour (1); Hsp101 null mutant.  Band showing at ~101kDa.</p>

WesternblotanalysisofArabidopsisArabidopsisthalinatissueshowingdetectionofHSP101proteinusingRabbitAnti-HSP101PolyclonalAntibody(SPC-304).Load:2µg.PrimaryAntibody:RabbitAnti-HSP101PolyclonalAntibody(SPC-304)at1:1000for1houratRT.SecondaryAntibody:HRP-conjugatedGoatAnti-Rabbitat1:10000.Fromlefttoright:ArabidopsisthalianaWTstressedat38°Cfor1.5hour(1);Hsp101nullmutant. Bandshowingat~101kDa.

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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。