StressMarq/StressXpress®HSC70 ELISA试剂盒/SKT-106-96/96孔
商品编号：
SKT-106-96
市场价：
¥12880.00
美元价：
7728.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | HSC70ELISAkit |
| Description | ColorimetricdetectionofHSC70 |
| SpeciesReactivity | Human |
| Platform | Microplate |
| SampleTypes | Celllysates,Serum,Tissue |
| DetectionMethod | ColorimetricAssay |
| AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
| Utility | ELISAkitusedtoquantitateHSC70concentrationinsamples. |
| Sensitivity | 1.54ng/ml |
| AssayRange | 2.34-150ng/mL |
| IncubationTime | 30minutes |
| NumberofSamples | 40samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||||||||
| ProductType | ELISAKits | ||||||||||||||||||||||||||||||||||||
| AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateatroomtemperature(20-25°C)for1hour.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatroomtemperaturefor1hour.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm. | ||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | HSC70ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-106) |
BIOLOGicalDescription
| AlternativeNames | HSC54ELISAKit,HSC71ELISAKit,HSC73ELISAKit,HSP71ELISAKit,HSP73ELISAKit,HSPA10ELISAKit,HSPA8ELISAKit,LAP1ELISAKit,NIP71ELISAKit |
| ResearchAreas | Cancer,HeatShock |
| ScientificBackground | HSP70genesencodeabundantheat-inducible70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast5O%identity(2).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(3).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingabilitythereforeresidedwithintheC-terminalhalf(4).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(5).Whencellsaresubjectedtometabolicstress(e.g.,heatshock)amemberoftheHSP70family,HSP70(HSP72),isexpressed;HSP70ishighlyrelatedtoHSC70(>90%sequenceidentity).ConstitutivelyexpressedHSC70rapidlyformsastablecomplexwiththehighlyinducIBLeHSP70incellsfollowingheatshock.TheinteractionofHSC70withHSP70isregulatedbyATP.Thesetwoheatshockproteinsmovetogetherinthecellexperiencingstress.FurThermore,researchonHSC70hasimplicatesitwitharoleinfacilitatingtherecoveryofcentrosomalstructureandfunctionafterheatshock(6). |
| References | 1.BrownC.L.,etal.(1993)J.CellBiol.,120(5):1101-1112. 2.BoorsteinW.R.,ZiegelhofferT.,andCraigE.A.(1993)J.Mol.Evol.38(1):1-17. 3.RothmanJ.(1989)Cell59:591-601. 4.DeLuca-Flaherty,etal.(1990)Cell62:875-887. 5.BorkP.,SanderC.,andValenciaA.(1992)Proc.NatlAcad.Sci.USA89:7290-7294. 6.BrownC.L.,etal.(1996)J.Biol.Chem.271(2):833-840. |
ProductImages
TypicalStandardCurvefortheHSC70ELISAkit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-106.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:2.34–150ng/mL.
ProductCitations(1)
OtherCitations
ChangesintheHeatShock70kDaProteinLevelinHumanNeutrophilsInducedbyHeatShock.
Boyko,A.A.,Vetchinin,S.S.,Sapozhnikov,A.M.andKovalenko,E.I.(2014)BioorgKhim.40(5):488-498.
PubMedID:25895348ReactivityHumanApplications:Neutrophil
品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
