STRESTMARQ/抗HSC70（鲑鱼）抗体/SPC-303D/100-µl-免疫在线蚂蚁淘旗下平台

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STRESTMARQ/抗HSC70（鲑鱼）抗体/SPC-303D/100-µl

商品编号： SPC-303D

品牌： StressMarq Biosciences

市场价： ¥6160.00

美元价： 3696.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

Product Name HSC70 (Salmon) Antibody

Description

Rabbit Anti-Salmon HSC70 (Salmon) Polyclonal

Species Reactivity Fish, Salmon, Three-spined stickleback (Gasterosteus aculeatus)

Applications WB

Antibody Dilution WB (1:1000); optimal dilutions for assays should be determined by the user.

Host Species Rabbit

Immunogen Species Salmon

Immunogen Synthetic peptide conjugated to KLH. The peptide target is specific to the HSC70 of salmonid fish.

Conjugates

Unconjugated

Properties

Storage Buffer	Lyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Rabbit antiserum
Clonality	Polyclonal
Specificity	Detects ~73kDa. The antibody detects HSC70, but not HSP70.
Cite This Product	StressMarq Biosciences Cat# SPC-303, RRID: AB_1501426

Biological Description

Alternative Names	HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody, HSPA8 Antibody
Research Areas	Cancer, Heat Shock
Cellular Localization	Cytoplasm, Melanosome
Accession Number	NP_001133079.1
Gene ID	100194521
Swiss Prot	B5DFX7
Scientific Background	HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (5).
References	1. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 2. Rothman J. (1989) Cell 59: 591-601. 3. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 4. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA 89: 7290-7294. 5. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.

Product Images

<p>Western blot analysis of Salmon tissue lysates showing detection of HSC70 protein using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-303). Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-303) at 1:1000.</p>

Western blot analysis of Salmon tissue lysates showing detection of HSC70 protein using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-303). Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-303) at 1:1000.

Product Citations (1)

Western Blot

Cold acclimation increases levels of some heat shock protein and sirtuin isoforms in threespine stickleback.

Teigen, L.E., Orczewska, J.I., McLaughlin, J. (2015) Comp Biochem Physiol A Mol Integr Physiol. 188:139-47.

PubMed ID: 26123780 Reactivity Gasterosteus aculeatus (Three-spined stickleback) Applications: Western Blot

品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

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