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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/HOP Protein/SPR-302A/50-µg
商品详细StressMarq/HOP Protein/SPR-302A/50-µg
StressMarq/HOP Protein/SPR-302A/50-µg
StressMarq/HOP Protein/SPR-302A/50-µg
商品编号: SPR-302A
市场价: ¥3200.00
美元价: 1920.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameHOPProtein
Description

HumanRecombinantHOPProtein

ApplicationsWB,SDS-PAGE,FunctionalAssay
ConcentrationLot/batchspecific.Seeincludeddatasheet.
ConjugatesHistag

Properties

StorageBuffer20mMHEPESbufferpH7.2,80mMNaCl,10%glycerol
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
Specificity~63kDa
CiteThisProductHumanRecombinantHOPProtein(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SPR-302)
CertificateofAnalysisThisproducthasbeencertified>90%pureusingSDSPAGEanalysis.4uMSPR-302,whenaddedto2uMSPR-300(Aha1)-activatedHSP90(2uM;His-taggedHSP90beta)in33mMHepespH7.2,30mMNaCl,5mMMgCl2,1mMDTT,1.5mMATPina100ulreactionat37degreesC,eliminatedallAha1-mediatedATPasestimulationaswellasintrinsicHSP90ATPaseactivity.(Thisisanenzyme-linkedATPregenerationassaytrackinglossofNADHabsorbanceat340nm).

BIOLOGicalDescription

AlternativeNamesHSPcochaperoneProtein
ResearchAreasCancer,HeatShock
CellularLocalizationCytoplasm,Nucleus
AccessionNumberNP_006810.1
GeneID10963
SwissProtP31948
ScientificBackgroundHop(HSP70/HSP90OrganizingProtein),orStress-inducedPhosphoprotein1(STI1)asitisalsoknown,isa60kDaproteinthatbelongstothelargegroupofco-chaperoneswhichregulateandassistthemajorchaperones.Itislocatedindiversecellularregionsandcanmovebetweenthecytoplasmandthenucleus.ItfunctionstoreversIBLylinktogethertheproteinchaperonesHSP70andHSP90.HOPcontainsthreetetratricopeptiderepeat(TPR)domains,TPR1,TPR2aandTPR2b.HSP70bindinghasbeenlocalizedtoTRP1andsp90bindinghavebeenlocalizedtoTPR2a(1).Ithasalsobeenfoundtomodulatethechaperoneactivitiesofthelinkedproteinsandpossibleinteractswithotherchaperonesandproteins.IthasalsobeenfoundtoparticipateinothercomplexesbesidestheHSP70/HSP90one(2).HOPiscloselyrelatedtohuman63kDaproteinthatissensitivetosimianvirusSV40transformation,andisrelatedtotheyeastheat-shock-responsiveSTI1geneproduct(3,4).
References1.FlomG.,BehalR.H.,RosenL.,ColeD.G.,JohnsonJ.L.(2007)BiochemJ.404(1):159-167.
2.HarstA.,LinH.,ObermannW.M.(2005)BiochemJ.387(pt3):789-796.
3.HonoreB.H.,etal.(1992)JBiolChem.267:8485-8491.
4.NicoletC.M.,etal.(1989)MolCellBio.9:3638-3646.

ProductImages

<p>SDS-PAGE of his-tagged human HOP protein (SPR-302).</p>

SDS-PAGEofhis-taggedhumanHOPprotein(SPR-302).

ProductCitations(3)

OtherCitations

HittingaMovingTarget:HowDoesanN-MethylGroupImpactBiologicalActivity?

Koay,Y.C.etal.(2016)ChemMedChem.11(8):881-92.

PubMedID:26805515Applications:ProteinBindingAssay

Dimerizationofaheatshockprotein90inhibitorenhancesinhibitoryactivity.

Wahyudi,H.,Wang,Y.andMcAlpine,S.R.(2014)OrgBiomolChem.12(5):765-73.

PubMedID:24323090Applications:ProteinBindingAssay

KnockdownofHopdownregulatesRhoCexpression,anddecreasespseudopodiaformationandmigrationincancercelllines.

Willmer,T.,Contu,L.,Blatch,G.L.,andEdkins,A.L.(2013)CancerLett.328(2):252-260.

PubMedID:23036489Applications:Actinco-sedimentationassay

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。