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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-HO-2 Antibody/SPC-212D-A390/100-µg
商品详细StressMarq/Anti-HO-2 Antibody/SPC-212D-A390/100-µg
StressMarq/Anti-HO-2 Antibody/SPC-212D-A390/100-µg
StressMarq/Anti-HO-2 Antibody/SPC-212D-A390/100-µg
商品编号: SPC-212D-A390
市场价: ¥6860.00
美元价: 4116.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameHO-2Antibody
Description

RabbitAnti-RatHO-2Polyclonal

SpeciesReactivityHuman,Mouse,Rat
ApplicationsWB,IHC,IP
AntibodyDilutionWB(1:500),IHC(1:1000),IP(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesRat
ImmunogenRatnativefull-lengthHO-2purifiedfromtestes
Concentration1mg/ml
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,0.09%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationProteinApurified
ClonalityPolyclonal
SpecificityDetects~36kDa.
CiteThisProductStressMarqBiosciencesCat#SPC-212,RRID:AB_2704325
CertificateofAnalysis2µg/mlofSPC-212wassufficientfordetectionofHO-2in20µgofRatbrainlysatebycolorimetricimmunoblotanalysisusingGoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesHemeoxygenase2Antibody,Hemeoxygenase(decycling)2Antibody,Hemeoxygenase(decyclizing)2Antibody,HMOX2Antibody,HMOX2Antibody,HMOX2_HUMANAntibody,HO2Antibody,HO2antibody
ResearchAreasCancer,OxidativeStress
CellularLocalizationEndoplasmicReticulum,Microsome
AccessionNumberNP_077363.1
GeneID79239
SwissProtP23711
ScientificBackgroundHeme-oxygenaseisaubiquitousenzymethatcatalyzestheinitialandrate-limitingstepsinhemecatabolismyieldingequimolaramountsofbiliverdin,ironandcarbonmonoxide.Biliverdinissubsequentlyconvertedtobilirubinandthefreeironissequesteredtoferritin(1).Theseproductshaveimportantphysiologicaleffectsascarbonmonoxideisapotentvasodilator;biliverdinandbilirubinarepotentantioxidants;andthefreeironincreasesoxidativestressandregulatestheexpressionofmanymRNAs(2).Therearethreeisoformsofheme-oxygenase,HO-1,HO-2andHO-3;howeverHO-1andHO-2arethemajorisoformsastheybothhavebeenidentifiedinmammals(3).HO-1,alsoknownasheatshockprotein32,isaninducIBLeisoformactivatedbymostoxidativestressinducers,cytokines,inflammatoryagentsandheatshock.HO-2isaconstitutiveisoformwhichisexpressedunderhomeostaticconditions.HO-1isalsoconsideredtobeacytoprotectivefactorinthatfreehemeishighlyreactiveandcytotoxic,andsecondly,carbonmonoxideisamediatorinhibitingtheinflammatoryprocessandbilirubinisascavengerforreactiveoxygen,bothofwhicharetheendproductsofhemecatalyzation(4).IthasalsobeenshownthatHO-1deficiencymaycausereducedstressdefense,apro-inflammatorytendency(5),susceptibilitytoatheroscleroticlesionformation(6),endothelialcellinjury,andgrowthretardation(7).Up-regulationofHO-1isthereforesaidtobeoneofthemajordefensemechanismsofoxidativestress(4).
References1.FrohM.etal.(2007)WorldJ.Gastroentereol13(25):3478-86.
2.ElbirtK.K.andBonkovskyH.L.(1999)ProcAssocAmPhysicians111(5):348-47.
3.MainesM.D.,TrakshelG.M.,andKuttyR.K.(1986)JBiolChem261:411–419.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody (SPC-212). Tissue: Spinal cord. Species: Rat. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody (SPC-212) at 1:1000.</p>

ImmunohistochemistryanalysisusingRabbitAnti-HO-2PolyclonalAntibody(SPC-212).Tissue:Spinalcord.Species:Rat.PrimaryAntibody:RabbitAnti-HO-2PolyclonalAntibody(SPC-212)at1:1000.

<p>Western blot analysis of Rat Brain cell lysates showing detection of HO-2 protein using Rabbit Anti-HO-2 Polyclonal Antibody (SPC-212). Lane 1: Rat Brain lysate. Lane 2: Purified HO-2. Load: 10 µg. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody (SPC-212) at 1:1000.</p>

WesternblotanalysisofRatBraincelllysatesshowingdetectionofHO-2proteinusingRabbitAnti-HO-2PolyclonalAntibody(SPC-212).Lane1:RatBrainlysate.Lane2:PurifiedHO-2.Load:10µg.PrimaryAntibody:RabbitAnti-HO-2PolyclonalAntibody(SPC-212)at1:1000.

ProductCitations(0)

Currentlytherearenocitationsforthisproduct.

 ATTO390
Overview:
  • Highfluorescenceyield
  • LargeStokes-shift(89nm)
  • GoodphotostABIlity
  • Moderatelyhydrophilic
  • Goodsolubilityinpolarsolvents
  • Coumarinderivate,uncharged
  • Lowmolarmass:343.42g/mol 

ATTO390Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission SpectraOpticalProperties:

λex =390nm

λem=479nm

εmax=2.4×104

Φf= 0.90

τfl =5.0ns

Brightness= 21.6

Laser =365or405nm

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。