StressMarq/StressXpress®HO-1 ELISA试剂盒/SKT-111-96/96孔
商品编号：
SKT-111-96
市场价：
¥10280.00
美元价：
6168.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | HO-1ELISAKit |
| Description | Colorimetricdetectionofhemeoxygenase1 |
| SpeciesReactivity | Human |
| Platform | Microplate |
| SampleTypes | Celllysates,Serum,Tissue |
| DetectionMethod | ColorimetricAssay |
| AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
| Utility | ELISAkitusedtoquantitateHO-1concentrationinsamples. |
| Sensitivity | 0.21ng/ml |
| AssayRange | 0.781-50ng/mL |
| IncubationTime | 30minutes |
| NumberofSamples | 40samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | |||||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | |||||||||||||||||||||||||||||||||||||||
| ProductType | ELISAKits | |||||||||||||||||||||||||||||||||||||||
| AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add50µLofPre-TreatmentBuffertoallsampleandstandardwells.3.Add50µLofStandardandsampletoappropriatewells.4.CoverplatewithPlateSealerandincubateatroomtemperature(20-25°C)for2hours.5.Washplatefourtimeswith1XWashBuffer.6.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.7.CoverplatewithPlateSealerandincubateatroomtemperaturefor1hour.8.Washplatefourtimeswith1XWashBufferasdescribedinstep5.9.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.10.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.11.Washplatefourtimeswith1XWashBufferasdescribedinstep5.12.Add100µLofTMBSubstratetoeachwell.13.Developtheplateinthedarkatroomtemperaturefor30minutes.14.Stopreactionbyadding100µLofStopSolutiontoeachwell.15.Measureabsorbanceonaplatereaderat450nm. | |||||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | HO-1ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-111) |
BIOLOGicalDescription
| AlternativeNames | Hemeoxygenase1ELISAKit,HemoxELISAKit,HMOX1ELISAKit,HO1ELISAKit,HO1ELISAKit,HSP32ELISAKit |
| ResearchAreas | Cancer,OxidativeStress |
| ScientificBackground | Heme-oxygenaseisaubiquitousenzymethatcatalyzestheinitialandrate-limitingstepsinhemecatabolismyieldingequimolaramountsofbiliverdin,ironandcarbonmonoxide.Biliverdinissubsequentlyconvertedtobilirubinandthefreeironissequesteredtoferritin(1).Theseproductshaveimportantphysiologicaleffectsascarbonmonoxideisapotentvasodilator;biliverdinandbilirubinarepotentantioxidants;andthefreeironincreasesoxidativestressandregulatestheexpressionofmanymRNAs(2).Therearethreeisoformsofheme-oxygenase,HO-1,HO-2andHO-3;howeverHO-1andHO-2arethemajorisoformsastheybothhavebeenidentifiedinmammals(3).HO-1,alsoknownasheatshockprotein32,isaninducIBLeisoformactivatedbymostoxidativestressinducers,cytokines,inflammatoryagentsandheatshock.HO-2isaconstitutiveisoformwhichisexpressedunderhomeostaticconditions.HO-1isalsoconsideredtobeacytoprotectivefactorinthatfreehemeishighlyreactiveandcytotoxic,andsecondly,carbonmonoxideisamediatorinhibitingtheinflammatoryprocessandbilirubinisascavengerforreactiveoxygen,bothofwhicharetheendproductsofhemecatalyzation(4).IthasalsobeenshownthatHO-1deficiencymaycausereducedstressdefense,apro-inflammatorytendency(5),susceptibilitytoatheroscleroticlesionformation(6),endothelialcellinjury,andgrowthretardation(7).Up-regulationofHO-1isthereforesaidtobeoneofthemajordefensemechanismsofoxidativestress(4). |
| References | 1.FrohM.etal.(2007)WorldJ.Gastroentereol13(25):3478-86. 2.ElbirtK.K.andBonkovskyH.L.(1999)ProcAssocAmPhysicians111(5):348-47. 3.MainesM.D.,TrakshelG.M.,andKuttyR.K.(1986)JBiolChem261:411–419. 4.BrydunA.,etal.(2007)HypertensRes30(4):341-8. 5.PossK.D.andTonegawaS.(1997).ProcNatlAcadSciUSA.94:10925–10930. 6.YetS.F.,etal.(2003)FASEBJ.17:1759–1761. 7.YachieA.,etal.(1999)JClinInvest.103:129–135. |
ProductImages
TypicalStandardCurvefortheHO-1ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-111.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.781–50ng/mL.
ProductCitations(1)
OtherCitations
Protectiveeffectofremotelimbpostconditioningviaupregulationofheme-oxygenase-1/BDNFpathwayinratmodelofcerebralischemicreperfusioninjury.
Ramagiri,S.andTaliyan,R.(2017)BrainRes.[Epubaheadofprint].
PubMedID:28535981ReactivityRatApplications:Brain
品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
