StressMarq/Anti-HDEL Antibody [2E7]/SMC-175D-BI/100-µg-免疫在线蚂蚁淘旗下平台

当前位置：首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-HDEL Antibody [2E7]/SMC-175D-BI/100-µg

商品详细StressMarq/Anti-HDEL Antibody [2E7]/SMC-175D-BI/100-µg

StressMarq/Anti-HDEL Antibody [2E7]/SMC-175D-BI/100-µg

商品编号： SMC-175D-BI

品牌： StressMarq Biosciences

市场价： ¥6940.00

美元价： 4164.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Overview:

Product Name HDEL Antibody

Description

Mouse Anti-Yeast HDEL Monoclonal IgG2b

Species Reactivity Barnyard Grass (Echinochloa), Beet (Beta vulgaris), Cotton (Gossypium), Fruit Fly (Drosophila melanogaster), Grass (Sorghum), Mung Bean (Vigna radiata), Plant, Wheat (Triticum spp.), Yeast, Yeast (Saccharomyces cerevisiae)

Applications WB, ICC/IF

Antibody Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Host Species Mouse

Immunogen Species Yeast

Immunogen Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip

Concentration 1 mg/ml

Conjugates

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Protein G Purified
Clonality	Monoclonal
Clone Number	2E7
Isotype	IgG2b
Specificity	Detects ~78kDa.
Cite This Product	StressMarq Biosciences Cat# SMC-175, RRID: AB_10556216
Certificate of Analysis	1 µg/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names	H-D-E-L (his-asp-glu-leu) Antibody, endoplasmic reticulum Antibody, luminal ER protein retention Antibody, KDELR1 Antibody, Endoplasmic reticulum retention signal Antibody
Research Areas	Cancer, Heat Shock, Cell Signaling, Protein Trafficking, Trafficking
Cellular Localization	Endoplasmic Reticulum
Scientific Background	HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
References	1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684. 2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697. 3. Entrez Gene: HDEL, Gene ID: 10945 4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.

Product Images

Currently there are no images for this product

Product Citations (0)

Currently there are no citations for this product.

Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

公司介绍