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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-HDEL Antibody [2E7]/SMC-175C/25-µg
商品详细StressMarq/Anti-HDEL Antibody [2E7]/SMC-175C/25-µg
StressMarq/Anti-HDEL Antibody [2E7]/SMC-175C/25-µg
StressMarq/Anti-HDEL Antibody [2E7]/SMC-175C/25-µg
商品编号: SMC-175C
市场价: ¥3200.00
美元价: 1920.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameHDELAntibody
Description

MouseAnti-YeastHDELMonoclonalIgG2b

SpeciesReactivityBarnyardGrass(Echinochloa),Beet(Betavulgaris),Cotton(Gossypium),FruitFly(Drosophilamelanogaster),Grass(Sorghum),MungBean(VignarADIata),Plant,Wheat(Triticumspp.),Yeast,Yeast(Saccharomycescerevisiae)
ApplicationsWB,ICC/IF
AntibodyDilutionWB(1:1000),ICC/IF(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesMouse
ImmunogenSpeciesYeast
ImmunogenRaisedagainstasyntheticHDELpeptidecorrespondingtotheC-terminusofyeastBip
Concentration1mg/ml
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,0.09%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationProteinGPurified
ClonalityMonoclonal
CloneNumber2E7
IsotypeIgG2b
SpecificityDetects~78kDa.
CiteThisProductStressMarqBiosciencesCat#SMC-175,RRID:AB_10556216
CertificateofAnalysis1µg/mlofSMC-175wassufficientfordetectionofHDEL-containingproteinsin10µgofS.cerevisiaelysatebycolorimetricimmunoblotanalysisusingGoatanti-mouseIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesH-D-E-L(his-asp-glu-leu)Antibody,endoplasmicreticulumAntibody,luminalERproteinretentionAntibody,KDELR1Antibody,EndoplasmicreticulumretentionsignalAntibody
ResearchAreasCancer,HeatShock,CellSignaling,ProteinTrafficking,Trafficking
CellularLocalizationEndoplasmicReticulum
ScientificBackgroundHSP70familycomprisesfourhighlyconservedproteins,HSP70,HSC70,GRP75andGRP78,whichserveavarietyofroles.Theyactasmolecularchaperones,facilitatingtheassemblyofmulti-proteincomplexes;participateinthetranslocationofpolypeptidesacrosscellmembranesandtothenucleus;andaidintheproperfoldingofnascentpolypeptidechains(1,2).GRP78islocalizedintheendoplasmicreticulum(ER),whereitreceivesimportedsecretoryproteinsandisinvolvedinthefoldingandtranslocationofnascentpeptidechains(2).SortingoftheseproteinsisdependentonaC-terminaltetrapeptidesignal,usuallyKDELinanimalcells,andHDELinS.cerevisiae(3).The2E7clonerecognizestheC-terminalpeptideHDEL,acommonversionoftheendoplasmicreticulumretentionsignalfoundinyeast,plant,nematodeandotherERproteins.2E7specificallystainsHDELproteinsinbarnyardgrass,beet,cotton,mungbean,sorghumandwheat(4).
References1.MayerM.P.,andBukauB.(2005)CellMolLifeSci.62(6):670-684.
2.LuoS.,MaoC.,LeeB.,andLeeA.S.(2006)MolCellBiol.26(15):5688-5697.
3.EntrezGene:HDEL,GeneID:10945
4.NapierR.M.,etal.(1992)JCellSci.102:261-271.

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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。