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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-HA tag Antibody [HA.C5]/SMC-260D-A488/100-µl
商品详细StressMarq/Anti-HA tag Antibody [HA.C5]/SMC-260D-A488/100-µl
StressMarq/Anti-HA tag Antibody [HA.C5]/SMC-260D-A488/100-µl
StressMarq/Anti-HA tag Antibody [HA.C5]/SMC-260D-A488/100-µl
商品编号: SMC-260D-A488
市场价: ¥6040.00
美元价: 3624.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameHAtagAntibody
Description

MouseAnti-HAtagMonoclonalIgG3

SpeciesReactivitySpeciesIndependent
ApplicationsWB,ICC/IF,IP
AntibodyDilutionWB(1:1000),ICC/IF(1:400),IP(1:400);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesMouse
ImmunogenSpeciesHuman
ImmunogenAsyntheticpeptidefrominfluenzahemagglutininepitope(YPYDVPDYA)coupledtoKLH.
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBS,pH7.4,50%glyercol,0.05%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
ClonalityMonoclonal
CloneNumberHA.C5
IsotypeIgG3
SpecificityHA-tagmonoclonalantibody.RecognizesHA-taggedproteinsoverexpressedincells,includingbothamino-orcarboxy-terminioftargetedproteinsintransfectedmammaliancells.
CiteThisProductStressMarqBiosciencesCat#SMC-260,RRID:AB_2699826
CertificateofAnalysisA1:1000dilutionofSMC-260wassufficientfordetectionofHATagin10µgofHumanHek293celllysatesbyECLimmunoblotanalysisusingGoatAnti-MouseIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesHAepitopetagantibody,HA1antibody,HA2antibody,hemagglutininantibody,HemagglutininHA1chainantibody,HemagglutininHA2chainantibody
ResearchAreasTagsandCellMarkers
ScientificBackgroundHumaninfluenzahemagglutinin(HA)isasurfaceglycoproteinrequiredfortheinfectivityofthehumanvirus.TheHAtagisderivedfromtheHAmoleculecorrespondingtoaminoacids98-106andhasbeenextensivelyusedasageneralepitopetaginexpressionvectors.ManyrecombinantproteinshavebeenengineeredtoexpresstheHAtag,whichdoesnotappeartointerferewiththebioactivityorthebiodistributionoftherecombinantprotein.Thistagfacilitatesthedetection,isolation,andpurificationoftheproteins.
References1.Schembri,L.etal.(2007)NatureMethods4(2):107–108.

ProductImages

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260). Secondary Antibody: APC Goat Anti-Mouse (red).</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-HATagMonoclonalAntibody,CloneHA.C5(SMC-260).PrimaryAntibody:MouseAnti-HATagMonoclonalAntibody(SMC-260).SecondaryAntibody:APCGoatAnti-Mouse(red).

<p>Western Blot analysis of Human Hek293 cell lysates showing detection of HA Tag protein using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Lane 1: Human 293T cells (Untransfected Control). Lane 2: Human 293T cells transfected with HA-Tag Vector.. Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260) at 1:1000.</p>

WesternBlotanalysisofHumanHek293celllysatesshowingdetectionofHATagproteinusingMouseAnti-HATagMonoclonalAntibody,CloneHA.C5(SMC-260).Lane1:Human293Tcells(UntransfectedControl).Lane2:Human293TcellstransfectedwithHA-TagVector..PrimaryAntibody:MouseAnti-HATagMonoclonalAntibody(SMC-260)at1:1000.

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 ATTO488
Overview:
  • Highfluorescenceyield
  • HighphotostABIlity
  • Veryhydrophilic
  • Excellentsolubilityinwater
  • Verylittleaggregation
  • Newdyewithnetchargeof-1
  • MolarMass:804g/mol 

ATTO488Datasheet

  ATTO 488 Fluorophore Excitation and Emission SpectraOpticalProperties:

λex =501nm

λem=523nm

εmax=9.0×104

Φf= 0.80

τfl =4.1ns

Brightness= 72

Laser = 488 nm

Filterset = FITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。