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当前位置： 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-HA tag Antibody [HA.C5]/SMC-260D-A390/100-µl
商品详细StressMarq/Anti-HA tag Antibody [HA.C5]/SMC-260D-A390/100-µl
StressMarq/Anti-HA tag Antibody [HA.C5]/SMC-260D-A390/100-µl
StressMarq/Anti-HA tag Antibody [HA.C5]/SMC-260D-A390/100-µl
商品编号： SMC-260D-A390
市场价： ¥6060.00
美元价： 3636.00
产地： 美国(厂家直采)
公司：
产品分类： 酸碱缓冲液
公司分类： acid_base_buffer_solution
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name HA tag Antibody
Description

Mouse Anti-HA tag Monoclonal IgG3

Species Reactivity Species Independent
Applications WB, ICC/IF, IP
Antibody Dilution WB (1:1000), ICC/IF (1:400), IP (1:400); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Human
Immunogen A synthetic peptide from influenza hemagglutinin epitope (YPYDVPDYA) coupled to KLH.
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS, pH 7.4, 50% glyercol, 0.05% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Clonality Monoclonal
Clone Number HA.C5
Isotype IgG3
Specificity HA-tag monoclonal antibody. Recognizes HA-tagged proteins overexpressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Cite This Product StressMarq Biosciences Cat# SMC-260, RRID: AB_2699826
Certificate of Analysis A 1:1000 dilution of SMC-260 was sufficient for detection of HA Tag in 10 µg of Human Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names HA epitope tag antibody, HA1 antibody, HA2 antibody, hemagglutinin antibody, Hemagglutinin HA1 chain antibody, Hemagglutinin HA2 chain antibody
Research Areas Tags and Cell Markers
Scientific Background Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 and has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
References 1. Schembri, L. et al. (2007) Nature Methods 4 (2): 107–108.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260). Secondary Antibody: APC Goat Anti-Mouse (red).</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260). Secondary Antibody: APC Goat Anti-Mouse (red).

<p>Western Blot analysis of Human Hek293 cell lysates showing detection of HA Tag protein using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Lane 1: Human 293T cells (Untransfected Control). Lane 2: Human 293T cells transfected with HA-Tag Vector.. Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260) at 1:1000.</p>

Western Blot analysis of Human Hek293 cell lysates showing detection of HA Tag protein using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Lane 1: Human 293T cells (Untransfected Control). Lane 2: Human 293T cells transfected with HA-Tag Vector.. Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260) at 1:1000.

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  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol 

ATTO 390 Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission SpectraOptical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

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StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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