StressMarq/StressXpress®谷胱甘肽S-转移酶活性试剂盒/SKT-203/96井
商品编号：
SKT-203
市场价：
¥7180.00
美元价：
4308.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | GlutathioneS-TransferaseActivityKit |
| Description | QuantitativefluorometricmeasurementoftheactivityofGST |
| SpeciesReactivity | Human,Fish,Mollusk,Oyster,Toadfish(Batrachoididae) |
| Platform | Microplate |
| SampleTypes | Celllysates,EDTAPlasma,HeparinPlasma,Serum,Urine |
| DetectionMethod | FluorometricAssay |
| AssayType | DirectEnzymeActivityAssay |
| Utility | FluorometricassayusedtoquantitativelymeasuretheactivityofglutathioneS-transferaseinsamples. |
| Sensitivity | 2.70mU/ml |
| AssayRange | 7.81-500mU/ml |
| Precision | IntraAssayPrecision:Fourserumsampleswerediluted1:2withAssayBufferandruninreplicatesof16inanassay.ThemeanandprecisionofthecalculatedGSTactivitieswere:Sample1-315.9mU/mL,4.6%CVSample2-221.2mU/mL,5.6%CVSample3-88.2mU/mL,4.2%CVSample4-22.7mU/mL,6.6%CVInterAssayPrecision:Fourserumsampleswerediluted1:2withAssayBufferandruninduplicatesintwentyassaysovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedGSTactivitieswere:Sample1-291.7mU/mL,12.6%CVSample2-218.5mU/mL,11.0%CVSample3-89.6mU/mL,10.4%CVSample4-23.0mU/mL,15.9%CV |
| IncubationTime | 30minutes |
| NumberofSamples | 40samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | |||||||||||||||||||||
| ShippingTemperature | BlueIce | |||||||||||||||||||||
| ProductType | ActivityKits | |||||||||||||||||||||
| AssayOverview | TheGlutathioneS-TransferaseActivitykitisdesignedtoquantitativelymeasuretheactivityofGSTpresentinavarietyofsamples.AGSTstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.Thekitutilizesanon-fluorescentmoleculethatisasubstratefortheGSTenzymewhichcovalentlyattachestoglutathione(GSH)toyieldahighlyfluorescentproduct.MixingthesampleorstandardwiththesuppliedDetectionReagentandGSHandincubatingatroomtemperaturefor30minutesyieldsafluorescentproductwhichisreadat460nminafluorescentplatereaderwithexcitationat390nm.TheactivityoftheGSTinthesampleiscalculated,aftermakingasuitablecorrectionforanydilutionofthesample,usingsoftwareavailablewithmostfluorescenceplatereaders.Wehaveprovideda96wellplateformeasurementbutthisassayisadaptableforhigherdensityplateformats.TheendusershouldensurethattheirHTSblackplateissuitableforusewiththesereagentspriortorunningsamples. | |||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | GlutathioneS-TransferaseActivityKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-203) |
BIOLOGicalDescription
| AlternativeNames | γ-L-Glutamyl-L-cysteinylglycineActivityKit(2S)-2-Amino-5-[[(2R)-1-(carboxymethylamino)-1-oxo-3-sulfanylpropan-2-yl]amino]-5-oxopentanoicacidActivityKit |
| ResearchAreas | Cancer,OxidativeStress |
| ScientificBackground | TheGlutathioneS-Transferase(GST)familyofisozymesfunctiontodetoxifyandneutralizeawidevarietyofelectrophilicmoleculesbymediatingtheirconjugationwithreducedglutathione(1).HumanGSTsareencodedby5genefamilies,expressinginalmostalltissuesasfourcytosolicandonemicrosomalforms.Dividingthefamilybyisoelectricpoints,thebasicalpha(pI8–11),theneutralmu(pI5–7)andacidicpi(pH<5) classes are populated by additional subclasses, each isozyme displaying differential specificity for given electrophilic molecules (2). Given its pivotal role in ameliorating oxidative stress/damage, GST activity has been repeatedly investigated as a bioMarkerforarthritis,asthma,COPD,andmultipleformsofcancer,aswellasanenvironmentalmarker(3-7).ExaminationofGSTisoformsandactivityinhumancancers,tumorsandtumorcelllineshasrevealedthepredominanceoftheacidicpiclass.FurThermore,thisactivityisthoughttosubstantiallycontributetotheinnateoracquiredresistanceofspecificneoplasmstoanticancertherapy(8,9). |
| References | 1.HABIg,W,etal.J.Biol.Chem.1974249(22):7130-7139. 2.Cook,JA,etal.CancerRes.199151:1606-1612. 3.Dalle-Donne,I,etal.200652(4):601-623. 4.Surapneni,KM&VSCGopan.Ind.J.Clin.Biochem.200823(1):41.44. 5.Mohan,SK&OVenkataramana.Ind.J.Med.Sci.200761:9-14. 6.Ferrandina,G.,etal.Ann.Oncol.19978:343-350. 7.Otitoju,O&Onwarah,INE.AfricanJ.Biotech.20076(12)1455-1459. 8.Shea,TC,etal.CancerRes.198848:527-533. 9.Shea,TC,etal.CancerRes.199050:6848-6853. |
