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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-GLUT1 Antibody/SPC-1295D-P594/100-µl
商品详细StressMarq/Anti-GLUT1 Antibody/SPC-1295D-P594/100-µl
StressMarq/Anti-GLUT1 Antibody/SPC-1295D-P594/100-µl
StressMarq/Anti-GLUT1 Antibody/SPC-1295D-P594/100-µl
商品编号: SPC-1295D-P594
市场价: ¥6180.00
美元价: 3708.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name GLUT1 Antibody
Description

Rabbit Anti-Human GLUT1 Polyclonal

Species Reactivity Human, Mouse, Rat
Applications WB, IHC, ELISA
Antibody Dilution WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:40000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthesized peptide derived from human GLUT1.
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Isotype IgG
Specificity Detects ~54kDa, endogenous levels of total GLUT1 protein.
Cite This Product StressMarq Biosciences Cat# SPC-1295, RRID: AB_2713115
Certificate of Analysis A 1:1000 dilution of SPC-1295 was sufficient for detection of GLUT1 in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) Antibody, CSE Antibody, DYT17 Antibody, DYT18 Antibody, DYT9 Antibody, EIG12 Antibody, erythrocyte/brain Antibody, Erythrocyte/hepatoma glucose transporter Antibody, facilitated glucose transporter member 1 Antibody, Glucose transporter 1 Antibody, Glucose transporter type 1 Antibody, Glucose transporter type 1, erythrocyte/brain Antibody, GLUT Antibody, GLUT-1 Antibody, GLUT1 Antibody, GLUT1DS Antibody, GLUTB Antibody, GT1 Antibody, GTG1 Antibody, Gtg3 Antibody, GTR1_HUMAN Antibody, HepG2 glucose transporter Antibody, HTLVR Antibody, Human T cell leukemia virus (I and II) receptor Antibody, MGC141895 Antibody, MGC141896 Antibody, PED Antibody, RATGTG1 Antibody, Receptor for HTLV 1 and HTLV 2 Antibody, SLC2A1 Antibody, Solute carrier family 2 (facilitated glucose transporter), member 1 Antibody, Solute carrier family 2 Antibody, Solute carrier family 2, facilitated glucose transporter member 1 Antibody
Research Areas Cancer, Atherosclerosis, Cardiovascular System
Cellular Localization Cell membrane, Melanosome
Accession Number NP_006507.2
Gene ID 6513
Swiss Prot P11166
Scientific Background Glucose transporter 1 is a uniporter protein that in humans is encoded by the SLC2A1 gene, also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1). GLUT-1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55kDa and a 45 kDa. The 55kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier, whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake.
References 1. Mueckler M. et al. (1985) Science 229 (4717): 941–5.
2. Olson A.L. and Pessin J.E. (1996) Annual Review of Nutrition 16: 235–56.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295). Tissue: Brain Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295). Tissue: Brain Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Western blot analysis of Human Jurkat cell lysates showing detection of ~55kDa GLUT1 protein using Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295). Lane 1: Human Jurkat cells.  Lane 2: Human Jurkat cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295) at 1:1000. Predicted/Observed Size: ~55kDa.</p>

Western blot analysis of Human Jurkat cell lysates showing detection of ~55kDa GLUT1 protein using Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295). Lane 1: Human Jurkat cells. Lane 2: Human Jurkat cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295) at 1:1000. Predicted/Observed Size: ~55kDa.

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 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission SpectraOptical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。