应力MARQ/FCR1/SIH-180-1MG/1-mg-免疫在线蚂蚁淘旗下平台

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商品详细应力MARQ/FCR1/SIH-180-1MG/1-mg

应力MARQ/FCR1/SIH-180-1MG/1-mg

商品编号： SIH-180-1MG

品牌： StressMarq Biosciences

市场价： ¥8500.00

美元价： 5100.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName	FCR1
Description	FRETratiometricfluorescence-basedredoxsensor
MolecularFormula	C₃₄H₃₇N₇O₆
MolecularWeight	639

Properties

StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
ProductType	RedoxProbe
Solubility	SolubleinDMSO
Source	Synthetic
Appearance	OrangeSolid
SafetyPhrases	Classification:Caution:Substancenotyetfullytested.SafetyPhrases:S22-DonotbreathedustS24/25-AvoidcontactwithskinandeyesS36/37/39-Wearsuitableprotectiveclothing,glovesandeye/faceprotection
CiteThisProduct	FCR1(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SIH-180)

BIOLOGicalDescription

AlternativeNames	7-(Diethylamino)-N-((1r,4r)-4-(2-(10-ethyl-2,4-dioxo-4,10-dihydrobenzo[g]pteridin-3(2H)-yl)acetamido)cyclohexyl)-2-oxo-2H-chromene-3-carboxamide(FCR1)
ResearchAreas	Cancer,OxidativeStress
ScientificBackground	FCR1orflavincoumarinredoxsensor1,isanovelratiometricfluorescentredoxsensorutilizedinfluorescencelifetimeimagingmicroscopyandflowcytometry.Intheoxidizedform,excitationofFCR1at405nmresultsinagreenfluorescencewithmaxemissionsat525nm.Treatmentwithamildreducingagent(includingsodiumcyanoborohydride,DTT,andglutathione)reducestheflavintherebydecreasingthegreenfluorescenceintensity,andincreasingtheblue.Re-oxidationcanbeachievedusingmildoxidizingagents.Ingeneral,theFCR1probecanbeusedtoobservechangesinoxidativecapacitywithoutinterferencefrombackgroundeffects.
References	1.KaurA.,HaghighatbinM.A.,HoganC.F.,andNewE.J.(2015)Chem.Commun.Epub.

ProductImages

<p>Chemical structure and design of FCR1 (SIH-180), showing FRET processes in oxidised form. Inset: photographs of cuvettes of FCR1 in oxidised and reduced forms under 365 nm excitation. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

ChemicalstructureanddesignofFCR1(SIH-180),showingFRETprocessesinoxidisedform.Inset:photographsofcuvettesofFCR1inoxidisedandreducedformsunder365nmexcitation.ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

<p>Fluorescence behavior of FCR1 (SIH-180) in the oxidized (green) and reduced (blue) forms, using 10 µM. Excitation: 405 nm. Reduced Emission: 475 nm. Oxidized Emission: 520 nm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

FluorescencebehaviorofFCR1(SIH-180)intheoxidized(green)andreduced(blue)forms,using10µM.Excitation:405nm.ReducedEmission:475nm.OxidizedEmission:520nm.ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

<p>Two photon – confocal microscopy imaging of HeLa cells treated with FCR1 (SIH-180, 10 µM, 15 min, λex = 820 nm) and (a) N-acetyl cysteine (50 µM, 30 min), (b) vehicle control and (c) H2O2 (50 µM, 30 min) in blue and green channels. The pseudo colour ratio images indicate the ratio of emission intensity in the green channel to blue channel. Scale bar represents 20 µm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

Twophoton–confocalmicroscopyimagingofHeLacellstreatedwithFCR1(SIH-180,10µM,15min,λex=820nm)and(a)N-acetylcysteine(50µM,30min),(b)vehiclecontroland(c)H2O2(50µM,30min)inblueandgreenchannels.Thepseudocolourratioimagesindicatetheratioofemissionintensityinthegreenchanneltobluechannel.Scalebarrepresents20µm.ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

<p>Fluorescence lifetimes of the donor (420 – 470 nm) in HeLa cells treated with FCR1 (SIH-180, 10 µM, λex = 820 nm) and N-acetyl cysteine, vehicle control and H2O2. Pseudo-colour images represent mean lifetime. Scale bar represents 50 µm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

Fluorescencelifetimesofthedonor(420–470nm)inHeLacellstreatedwithFCR1(SIH-180,10µM,λex=820nm)andN-acetylcysteine,vehiclecontrolandH2O2.Pseudo-colourimagesrepresentmeanlifetime.Scalebarrepresents50µm.ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

<p>Flow cytometric studies of HeLa cells treated with FCR1 (SIH-180, 10 µM, λex = 405 nm) showing the fluorescence ratio (560 / 450 nm) when treated with N-acetyl cysteine (blue), vehicle control (green) and H2O2 (red). Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

FlowcytometricstudiesofHeLacellstreatedwithFCR1(SIH-180,10µM,λex=405nm)showingthefluorescenceratio(560/450nm)whentreatedwithN-acetylcysteine(blue),vehiclecontrol(green)andH2O2(red).ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

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品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

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