STRESTMARQ/抗Erk1/2抗体/SPC-120D-BI/100-µl-免疫在线蚂蚁淘旗下平台

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商品详细STRESTMARQ/抗Erk1/2抗体/SPC-120D-BI/100-µl

STRESTMARQ/抗Erk1/2抗体/SPC-120D-BI/100-µl

商品编号： SPC-120D-BI

品牌： StressMarq Biosciences

市场价： ¥5880.00

美元价： 3528.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName Erk1/2Antibody

Description

RabbitAnti-RatErk1/2Polyclonal

SpeciesReactivity Human,Mouse,Rat,Africanclawedfrog(Xenopuslaevis),Bovine,Chicken,FruitFly(Drosophilamelanogaster),Sheep

Applications WB,IHC,ICC/IF,FCM

AntibodyDilution WB(1:1000),IHC(1:100),ICC/IF(1:100),FCM(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Rabbit

ImmunogenSpecies Rat

Immunogen A35residuesyntheticpeptide,correspondingtoErk1MAPkinasewiththeCGGspacergroupaddedandthepeptidecoupledtoKLH.

Concentration 1mg/ml

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-ProteinComplex
Smallphycobiliprotein
Isolatedfromredalgae
Largestokesshift(195nm)
MolecularWeight:35kDa

PerCPDatasheet

PerCP Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =482nm

λ_em=677nm

ε_max=1.96x10⁶

Laser =488nm

PE/ATTO594

PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.

Overview:

Highfluorescenceyield
HighphotostABIlity
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation

PE/ATTO594Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

OpticalProperties:

λ_ex =535nm

λ_em =627 nm

Laser = 488to561nm

FITC(Fluorescein)

Overview:

Excellentfluorescencequantumyield
Highrateofphotobleaching
Goodsolubilityinwater
Broademissionspectrum
pHdependentspectra
Molecularformula:C₂₀H₁₂O₅
Molarmass:332.3g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =494 nm

λ_em=520 nm

ε_max=7.3×10⁴

Φ_f= 0.92

τ_fl =5.0ns

Brightness= 67.2

Laser = 488 nm

Filterset = FITC

ATTO700

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:575g/mol

ATTO700Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =700 nm

λ_em=719nm

ε_max=1.25×10⁵

Φ_f= 0.25

τ_fl =1.6ns

Brightness= 31.3

Laser =676nm

Filterset =Cy®5.5

ATTO680

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:631g/mol

ATTO680Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =680nm

λ_em=700 nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.7ns

Brightness=37.5

Laser =633 –676nm

Filterset =Cy®5.5

ATTO655

Overview:

Highfluorescenceyield
Highthermalandphotostability
Excellentozoneresistance
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:634g/mol

ATTO655Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =663nm

λ_em=684nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.8ns

Brightness= 37.5

Laser =633 –647nm

Filterset =Cy®5

ATTO633

Overview:

Highfluorescenceyield
Highthermalandphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
StableatpH4–11
Cationicdye,perchloratesalt
MolarMass:652.2g/mol

ATTO633Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =629nm

λ_em=657nm

ε_max=1.3×10⁵

Φ_f= 0.64

τ_fl =3.2ns

Brightness=83.2

Laser =633 nm

Filterset =Cy®5

ATTO594

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:1137g/mol

ATTO594Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

OpticalProperties:

λ_ex =601 nm

λ_em=627nm

ε_max=1.2×10⁵

Φ_f= 0.85

τ_fl =3.5ns

Brightness=102

Laser =594nm

Filterset =TexasRed®

ATTO565

Overview:

Highfluorescenceyield
Highthermalandphotostability
Goodsolubilityinpolarsolvents
Excellentsolubilityinwater
Verylittleaggregation
Rhodaminedyederivative
MolarMass:611g/mol

ATTO565Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =563nm

λ_em=592nm

ε_max=1.2×10⁵

Φ_f= 0.9

τ_fl =3.4n

Brightness=10

Laser =532nm

Filterset = TRITC

ATTO488

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:804g/mol

ATTO488Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =501nm

λ_em=523nm

ε_max=9.0×10⁴

Φ_f= 0.80

τ_fl =4.1ns

Brightness= 72

Laser = 488 nm

Filterset = FITC

ATTO390

Overview:

Highfluorescenceyield
LargeStokes-shift(89nm)
Goodphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
Coumarinderivate,uncharged
Lowmolarmass:343.42g/mol

ATTO390Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

OpticalProperties:

λ_ex =390nm

λ_em=479nm

ε_max=2.4×10⁴

Φ_f= 0.90

τ_fl =5.0ns

Brightness= 21.6

Laser =365or405nm

APC(Allophycocyanin)

Overview:

Highquantumyield
Largephycobiliprotein
6chromophorespermolecule
Isolatedfromredalgae
MolecularWeight:105kDa

APCDatasheet

APC Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =650nm

λ_em=660nm

ε_max=7.0×10⁵

Φ_f= 0.68

Brightness= 476

Laser =594or633 nm

Filterset =Cy®5

Streptavidin

Properties:

Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinity
Molecularweight:53kDa
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

StreptavidinDatasheet

Biotin

Properties:

BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity
Molarmass:244.31g/mol
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

BiotinDatasheet

HRP(HorserADIshperoxidase)

Properties:

Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
Readilycombineswithhydrogenperoxide(H₂O₂)toformHRP-H₂O₂complexwhichcanoxidizevarioushydrogendonors
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
Highturnoverrateenablesrapidgenerationofastrongsignal
44kDaglycoprotein
Extinctioncoefficient:100(403nm)
Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

AP(AlkalinePhosphatase)

Properties:

Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols
Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts
- Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts
Molecularweight:140kDa
Applications:Westernblot,immunohistochemistry,andELISA

APDatasheet

R-PE(R-Phycoerythrin)

Overview:

Broadexcitationspectrum
Highquantumyield
Photostable
Memberofthephycobiliproteinfamily
Isolatedfromredalgae
Excellentsolubilityinwater
MolecularWeight:250kDa

R-PEDatasheet

R-PE Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =565nm

λ_em=575nm

ε_max=2.0×10⁶

Φ_f= 0.84

Brightness=1.68x10³

Laser = 488to561nm

Filterset = TRITC

Properties

StorageBuffer	PBSpH7.4,50%glycerol,0.09%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	PeptideAffinityPurified
Clonality	Polyclonal
Specificity	Detects~44kda(ERK1)and~42kDa(ERK2).
CiteThisProduct	StressMarqBiosciencesCat#SPC-120,RRID:AB_2266080
CertificateofAnalysis	A1:1000dilutionofSPC-120wassufficientfordetectionofERK1/2in20µgofHeLacelllysatebyECLimmunoblotanalysis.

BIOLOGicalDescription

AlternativeNames	ERK1Antibody,ERK2Antibody,ERT1Antibody,ERT2Antibody,MAPkinase1Antibody,MAPkinase2Antibody,MAPK1Antibody,MAPK2Antibody,MAPK3Antibody,p38Antibody,p40Antibody,p41Antibody,p41mapkAntibody,p42MAPKAntibody,p44ERK1Antibody,p44MAPKAntibody,PRKM1PRKM2Antibody,PRKM3Antibody
ResearchAreas	Alzheimer"sDisease,CellSignaling,Neurodegeneration,Neuroscience,Phosphorylation,Post-translationalModifications
CellularLocalization	Cytoplasm,Nucleus
AccessionNumber	NP_059043.1
GeneID	50689
SwissProt	P21708
ScientificBackground	Theextracellularsignal-regulatedkinases1and2(ERK1andERK2),alsocalledp44andp42MAPkinases,aremembersoftheMitogenActivatedProteinKinase(MAPK)familyofproteinsfoundinalleukaryotes.Becausethe44kDaERK1andthe42kDaERK2arehighlyhomologousandbothfunctioninthesameproteinkinasecascade,thetwoproteinsareoftenreferredtocollectivelyasERK1/2orp44/p42MAPkinase(1).Theyarebothlocatedinthecytosolandmitochondria(2).WhiletheroleofcytosolERK1/2iswellstudiedandinvolvedinmultiplecellularfunctions(2),theroleofmitochondrialERK1/2remainspoorlyunderstood.BothERK1and2areactivatedbyMEK1orMEK2,bydualphosphorylationofathreonineandtyrosineresidueintheactivationloop(TEYmotif)(1,3).Eitherphosphorylationalonecaninduceanelectrophoreticmobilityshift,butbotharerequiredforactivationofthekinase.Thisdualphosphorylationisefficientlydetectedbyphosphorylationstate-specificantibodydirectedtothepTEpYmotif.Onceactivated,MAPkinasesphosphorylateabroadspectrumofsubstrates,includingcytoskeletalproteins,translationregulators,transcriptionfactors,andtheRskfamilyofproteinkinases(4).ERK1/2activationisgenerallythoughttoconferasurvivaladvantagetocells(5);howeverthereisincreasingevidencethatsuggeststhattheactivationofERK1/2alsocontributestocelldeathundercertainconditions(5).ERK1/2alsoisactivatedinneuronalandrenalepithelialcellsuponexposuretooxidativestressandtoxicantsordeprivationofgrowthfactors,andinhibitionoftheERKpathwayblocksapoptosis(5).
References	1.BoultonTG.etal.(1991)Biochemistry.30(1):278-86. 2.YoonS.,andSegerR.(2006)GrowthFactors24:21-44. 3.WolfG.(2005)AntioxidRedoxSignal7:1337-1345. 4.ChuerlandD.,MarmorG.,ShainskayaA.andSegerR.(2008)JBiolChem.Epub:http://www.jbc.org/cgi/doi/10.1074/jbc.M709030200 5.ZhuangS.,andSchnellmannR.G.(2006)JPharmacolExpTher319:991-997.

ProductImages

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal Antibody (SPC-120). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody (SPC-120) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-Erk1/2PolyclonalAntibody(SPC-120).Tissue:HeLaCells.Species:Human.Fixation:2%Formaldehydefor20minatRT.PrimaryAntibody:RabbitAnti-Erk1/2PolyclonalAntibody(SPC-120)at1:100for12hoursat4°C.SecondaryAntibody:APCGoatAnti-Rabbit(red)at1:200for2hoursatRT.Counterstain:DAPI(blue)nuclearstainat1:40000for2hoursatRT.Localization:Cytoplasm.Nucleus.Magnification:100x.(A)DAPI(blue)nuclearstain.(B)Anti-Erk1/2Antibody.(C)Composite.

<p>Western blot analysis of Human Cell line lysates showing detection of ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.</p>

WesternblotanalysisofHumanCelllinelysatesshowingdetectionofERK1proteinusingRabbitAnti-ERK1PolyclonalAntibody(SPC-120).Load:15µgprotein.Block:1.5%BSAfor30minutesatRT.PrimaryAntibody:RabbitAnti-ERK1PolyclonalAntibody(SPC-120)at1:1000for2hoursatRT.SecondaryAntibody:DonkeyAnti-RabbitIgG:HRPfor1houratRT.

<p>Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:25000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.</p>

ImmunohistochemistryanalysisusingRabbitAnti-ERK1PolyclonalAntibody(SPC-120).Tissue:Inflamedcolon.Species:Mouse.Fixation:Formalin.PrimaryAntibody:RabbitAnti-ERK1PolyclonalAntibody(SPC-120)at1:25000for12hoursat4°C.SecondaryAntibody:BiotinGoatAnti-Rabbitat1:2000for1houratRT.Counterstain:MethylGreenat200uLfor2minatRT.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-ERK1PolyclonalAntibody(SPC-120).Tissue:HaCaTcells.Species:Human.Fixation:Cold100%methanolat-20Cfor10minutes.PrimaryAntibody:RabbitAnti-ERK1PolyclonalAntibody(SPC-120)at1:100for12hoursat4°C.SecondaryAntibody:FITCGoatAnti-Rabbitat1:50for1-2hoursatRTindark.Localization:Cytoplasm.Nucleus.

<p>Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative Solution. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.</p>

ImmunohistochemistryanalysisusingRabbitAnti-ERK1PolyclonalAntibody(SPC-120).Tissue:backskin.Species:Mouse.Fixation:Bouin’sFixativeSolution.PrimaryAntibody:RabbitAnti-ERK1PolyclonalAntibody(SPC-120)at1:100for1houratRT.SecondaryAntibody:FITCGoatAnti-Rabbit(green)at1:50for1houratRT.Localization:Cytoplasm.

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-Erk1/2PolyclonalAntibody(SPC-120).Tissue:HeLaCells.Species:Human.Fixation:2%Formaldehydefor20minatRT.PrimaryAntibody:RabbitAnti-Erk1/2PolyclonalAntibody(SPC-120)at1:100for12hoursat4°C.SecondaryAntibody:FITCGoatAnti-Rabbit(green)at1:200for2hoursatRT.Counterstain:DAPI(blue)nuclearstainat1:40000for2hoursatRT.Localization:Cytoplasm.Nucleus.Magnification:20x.(A)DAPI(blue)nuclearstain.(B)Anti-Erk1/2Antibody.(C)Composite.

ProductCitations(5)

WesternBlot

Facilitatoryeffectsoffetuin-Aonatherosclerosis.

Naito,C.etal.(2016)Atherosclerosis.246:344-351

PubMedID:26828753ReactivityHumanApplications:WesternBlot

Hypoxia-drivensarcoplasmic/endoplasmicreticulumcalciumATPase2(SERCA2)downregulationdependsonlow-densitylipoproteinreceptor-relatedprotein1(LRP1)-signallingincardiomyocytes.

Revuelta-López,E.(2015)JMolCellCardiol.85(1):25-36.

PubMedID:25968337ReactivityMouseApplications:WesternBlot

LeptininducedGRP78expressionthroughthePI3K-mTORpathwayinneuronalcells.

Thon,M.,Hosoi,T.,Yoshii,M.andOzawa,K.(2014)SciRep.Articlenumber:7096.

PubMedID:25403445ReactivityHumanApplications:WesternBlot

RoleofHsp90inCpGODNmediatedimmunostimulationinavianmacrophages.

Bhat,A.,Gomis,S.,Potter,A.andTikoo,S.K.(2010)MolImmunol.47(6):1337-1346.

PubMedID:20096933ReactivityChickenApplications:WesternBlot

Immunohistochemistry

Involucrin-claudin-6taildeletionmutant(CDelta206)transgenicmice:amodelofdelayedepidermalpermeabilitybarrierformationandrepair.

Enikanolaiye,A.etal.(2010)DisModelMech.3(3-4):167-180.

PubMedID:20106878ReactivityMouseApplications:Immunohistochemistry

Biotin

Properties:

BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity
Molarmass:244.31g/mol
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

BiotinDatasheet

品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

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