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当前位置: 首页 > 产品中心 > DNA_buffer_solution > STRESTMARQ/抗ENaCβ抗体[7B8]/SMC-240D-A565/100-微克
商品详细STRESTMARQ/抗ENaCβ抗体[7B8]/SMC-240D-A565/100-微克
STRESTMARQ/抗ENaCβ抗体[7B8]/SMC-240D-A565/100-微克
STRESTMARQ/抗ENaCβ抗体[7B8]/SMC-240D-A565/100-微克
商品编号: SMC-240D-A565
市场价: ¥6840.00
美元价: 4104.00
产地: 美国(厂家直采)
公司:
产品分类: 上样缓冲液
公司分类: DNA_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameENaCbetaAntibody
Description

MouseAnti-RatENaCbetaMonoclonalIgG1

SpeciesReactivityMouse
ApplicationsWB,IHC
AntibodyDilutionWB(1:1000);IHC(1:150);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesMouse
ImmunogenSpeciesRat
ImmunogenSyntheticpeptidefromtheC-terminalofRatENaCbeta(aa.617-638)
Concentration1mg/ml
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,0.09%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationProteinGPurified
ClonalityMonoclonal
CloneNumber7B8
IsotypeIgG1
SpecificityDetects~87kDa.
CiteThisProductStressMarqBiosciencesCat#SMC-240,RRID:AB_2699538
CertificateofAnalysisA1:1000dilutionofSMC-240wassufficientfordetectionofENaCbetain15µgofMousewholekidneylysatebyECLimmunoblotanalysisusinggoatanti-mouseIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesSCNN1BAntibody,AmiloridesensitivesodiumchannelsubunitbetaAntibody,Amiloride-sensitivesodiumchannelbeta-subunitAntibody,BetaENaCAntibody,BetaNaCHAntibody,ENaCbetaAntibody,ENaCBAntibody,EpithelialNa(+)channelsubunitbetaAntibody,EpithelialNa+channelbetasubunitAntibody,EpithelialNa+channelsubunitbetaAntibody,Epithelialsodiumchannelbeta2subunitAntibody,Epithelialsodiumchannelbeta3subunitAntibody,Nonvoltagegatedsodiumchannel1betasubunitAntibody,Nonvoltagegatedsodiumchannel1subunitbetaAntibody,Nonvoltage-gatedsodiumchannel1betasubunitAntibody,SCNEBAntibody,SCNN1BAntibody,Sodiumchannelnonvoltagegated1beta(Liddlesyndrome)Antibody,Sodiumchannelnonvoltagegated1betaAntibody
ResearchAreasEpithelialSodiumChannels(ENaC),IonChannels,Neuroscience,SodiumChannels
CellularLocalizationApicalcellmembrane
AccessionNumberNP_036780
GeneID24767
SwissProtP37090
ScientificBackgroundTheEpithelialSodiumChannel(ENaC)isamembraneionchannelpermeabletoNa+ions.Itislocatedintheapicalplasmamembraneofepitheliainthekidneys,lung,colon,andothertissueswhereitplaysaroleintransepithelialNa+-iontransport(1).SpecificallyNa+transportviaENaCoccursacrossmanyepithelialsurfaces,andplaysakeyroleinregulatingsaltandwaterabsorption(2).ENaCsarecomposedofthreestructurallyrelatedsubunitsthatformatetramericchannel,alpha,beta,andgamma.Theexpressionofitsalphaandbetasubunitsisenhancedaskeratinocytesdifferentiate(3,4).Thebetaandgamma-ENaCsubunitsareessentialforedemafluidtoexertitsmaximaleffectonnetfluidabsorptionbydistallungepithelia(5).Andithasbeenconcludedthatthesubunitsaredifferentiallyexpressedintheretinaofmicewithocularhypertension,thereforetheup-regulationofalpha-ENaCproteinscouldserveasaprotectionmechanismagainstelevatedintraocularpressure(6).
References1.KakizoeY.,etal.(2009)JHpyertens.27(8):1679-1689.
2.GuY.(2008)JCellPhysiol.216(2):453-457.
3.BrunsJ.B.(2003)AmJPhysiolRenalPhysiol.285(4):F600-F609.
4.MauroT.,etal.(2002)JInvestDermatol.118(4):589-594.
5.EliasN.,etal.(2007)AmJPhysiolLungCellMolPhysiol.293(3):L537-45.
6.DykaF.M.,MayC.A.andEnzR.(2005)JNeuRochem.94(1):120-128.

ProductImages

<p>Immunohistochemistry analysis using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8 (SMC-240). Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody (SMC-240) at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.</p>

ImmunohistochemistryanalysisusingMouseAnti-ENaCbetaMonoclonalAntibody,Clone7B8(SMC-240).Tissue:Kidney(cortex).Species:Mouse.PrimaryAntibody:MouseAnti-ENaCbetaMonoclonalAntibody(SMC-240)at1:150.Localization:Collectingductprincipalcells.Magnification:60X.

<p>Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8 (SMC-240). Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 µg. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody (SMC-240) at 1:1000. Predicted/Observed Size: ~87kDa.</p>

WesternBlotanalysisofMouseWholekidneyhomogenatesshowingdetectionof~87kDaENaCbetaproteinusingMouseAnti-ENaCbetaMonoclonalAntibody,Clone7B8(SMC-240).Lane1:MolecularWeightLadder(MW).Lane2:Low-saltdiet.Lane3:Normal-saltdiet.Load:20µg.PrimaryAntibody:MouseAnti-ENaCbetaMonoclonalAntibody(SMC-240)at1:1000.Predicted/ObservedSize:~87kDa.

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 ATTO565
Overview:
  • Highfluorescenceyield
  • HighthermalandphotostABIlity
  • Goodsolubilityinpolarsolvents
  • Excellentsolubilityinwater
  • Verylittleaggregation
  • Rhodaminedyederivative
  • MolarMass:611g/mol

ATTO565Datasheet

 ATTO 565 Fluorophore Excitation and Emission SpectraOpticalProperties:

λex =563nm

λem=592nm

εmax=1.2×105

Φf= 0.9

τfl =3.4n

Brightness=10

Laser =532nm

Filterset = TRITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。