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当前位置: 首页 > 产品中心 > DNA_buffer_solution > STRESTMARQ/抗ENaCβ抗体/SPC-404D-A700/100-微克
商品详细STRESTMARQ/抗ENaCβ抗体/SPC-404D-A700/100-微克
STRESTMARQ/抗ENaCβ抗体/SPC-404D-A700/100-微克
STRESTMARQ/抗ENaCβ抗体/SPC-404D-A700/100-微克
商品编号: SPC-404D-A700
市场价: ¥7020.00
美元价: 4212.00
产地: 美国(厂家直采)
公司:
产品分类: 上样缓冲液
公司分类: DNA_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name ENaC Beta Antibody
Description

Rabbit Anti-Rat ENaC Beta Polyclonal

Species Reactivity Human, Mouse, Rat, African clawed frog (Xenopus laevis), Hamster
Applications WB, IHC, ICC/IF, IP
Antibody Dilution WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Rat
Immunogen Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2)
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein A purified
Clonality Polyclonal
Specificity Detects ~87kDa.
Cite This Product StressMarq Biosciences Cat# SPC-404, RRID: AB_10644173
Certificate of Analysis 1 µg/ml of SPC-404 was sufficient for detection of beta-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names SCNN1B Antibody, Amiloride sensitive sodium channel subunit beta Antibody, Amiloride-sensitive sodium channel beta-subunit Antibody, Beta ENaC Antibody, Beta NaCH Antibody, ENaC beta Antibody, ENaCB Antibody, Epithelial Na(+) channel subunit beta Antibody, Epithelial Na+ channel beta subunit Antibody, Epithelial Na+ channel subunit beta Antibody, Epithelial sodium channel beta 2 subunit Antibody, Epithelial sodium channel beta 3 subunit Antibody, Nonvoltage gated sodium channel 1 beta subunit Antibody, Nonvoltage gated sodium channel 1 subunit beta Antibody, Nonvoltage-gated sodium channel 1 beta subunit Antibody, SCNEB Antibody, SCNN 1B Antibody, Sodium channel nonvoltage gated 1 beta (Liddle syndrome) Antibody, Sodium channel nonvoltage gated 1 beta Antibody
Research Areas Epithelial Sodium Channels (ENaC), Ion Channels, Neuroscience, Sodium Channels
Cellular Localization Apical cell membrane
Accession Number NP_036780
Gene ID 24767
Swiss Prot P37090
Scientific Background The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
References 1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689.
2. Gu Y. (2008) J Cell Physiol. 216(2):453-457.
3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609.
4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594.
5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45.
6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody (SPC-404). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-404) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).</p>

Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody (SPC-404). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-404) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).

<p>Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-404). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-404) at 1:1000.</p>

Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-404). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-404) at 1:1000.

<p>Western blot analysis of Mouse mpkCCD cell lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-404). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-404) at 1:1000.</p>

Western blot analysis of Mouse mpkCCD cell lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-404). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-404) at 1:1000.

<p>Western blot analysis of Mouse kidney cortex showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-404). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-404) at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-8).</p>

Western blot analysis of Mouse kidney cortex showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-404). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-404) at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-8).

Product Citations (29)

Western Blot

Enac Regulation in the Kidney: the Role of Ankyrin G

Klemens, C.A. (2017) University of Pittsburgh. PhD Dissertation.

PubMed ID: N/A Reactivity Rat Applications: Western Blot

Derlin-1 promotes ubiquitination and degradation of epithelial sodium channel (ENaC).

You, H. et al. (2017) J Cell Sci. 130(6):1027-1036.

PubMed ID: 28137758 Reactivity Mouse Applications: Western Blot

Derlin-1 promotes ubiquitination and degradation of epithelial sodium channel (ENaC).

You, H. et al. (2017) J Cell Sci. 130(6):1027-1036.

PubMed ID: 28137758 Reactivity Human Applications: Western Blot

Ankyrin G Expression Regulates Apical Delivery of the Epithelial Sodium Channel (ENaC).

Klemens, C.A., Edinger, R.S., Kightlinger, L., Liu, X. and Butterworth, M.B. (2016) J Biol Chem. 292(1):375-385.

PubMed ID: 27895120 Reactivity Mouse Applications: Western Blot

Renal sodium transport in renin-deficient Dahl salt-sensitive rats.

Pavlov, T.S., Levchenko, V., Ilatovskaya, D.V., Moreno, C. and Staruschenko, A. (2016) J Renin Angiotensin Aldosterone Syst. 17(3).

PubMed ID: 27443990 Reactivity Rat Applications: Western Blot

(Pro)Renin Receptor Regulates Potassium Homeostasis through a Local Mechanism.

Xu, C. et al. (2016) Am J Physiol Renal Physiol. [Epub ahead of print]

PubMed ID: 27440776 Reactivity Rat Applications: Western Blot

SPAK and OSR1 play essential roles in potassium homeostasis through actions on the distal convoluted tubule.

Ferdaus, M.Z. et al. (2016) J Physiol. 594(17):4945-66.

PubMed ID: 27068441 Reactivity Mouse Applications: Western Blot

Renal tubular epithelial cell prorenin receptor regulates blood pressure and sodium transport.

Ramkumar, N. et al. (2016) Am J Physiol Renal Physiol. 311(1):F186-94.

PubMed ID: 27053687 Reactivity Mouse Applications: Western Blot

The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation.

Patel-Chamberlin, M. et al. (2016) PLoS One. 11(3):e0150918.

PubMed ID: 26963391 Reactivity Mouse Applications: Western Blot

The Thiazide-Sensitive Co-Transporter Promotes the Development of Sodium Retention in Mice with Diet-Induced Obesity.

Davies, M. R. P. et al. (2015) Kidney Blood Press Res. 40(5):509-519.

PubMed ID: 26418861 Reactivity Mouse Applications: Western Blot

Inhibition of Mitochondrial Complex-1 Prevents the Downregulation of NKCC2 and ENaCα in Obstructive Kidney Disease.

Zhang, Y. et al. (2015) Sci Rep. 5:12480.

PubMed ID: 26207612 Reactivity Mouse Applications: Western Blot

Compositions And Methods Of Use For (PRO)Renin Receptor.

Feng, Y. and Yang, T. (2015) United States Patent Application 20150025013.

PubMed ID: N/A Reactivity Rat Applications: Western Blot

Intersubunit conformational changes mediate epithelial sodium channel gating.

Collier, D.M., Tomkovicz, V.R., Peterson, Z.J., Benson, C.J., and Snyder, P.M. (2014) J Gen Physiol. 144(4):337-48.

PubMed ID: 25225551 Reactivity Human Applications: Western Blot

Role of Rho GDP-dissociation inhibitor alpha in control of ENaC-mediated sodium reabsorption.

Pavlov, T. S., Levchenko, V. and Staruschenko, A. (2014) J Biol Chem. 289(41):28651-9.

PubMed ID: 25164814 Reactivity Mouse Applications: Western Blot

Collecting duct specific knock-out of renin attenuates angiotensin-II induced hypertension.

Ramkumar, N. et al. (2014) Am J Physiol Renal Physiol. 307(8):F931-8.

PubMed ID: 25122048 Reactivity Mouse Applications: Western Blot

Novel Mechanisms of Sodium Retention in Obesity: Phosphorylation of NKCC2 and Regulation of SPAK/OSR1 by AMPK.

Davies, M. R. P. et al. (2014) Am J Physiol. 307(1):F96-F106.

PubMed ID: 24808538 Reactivity Mouse Applications: Western Blot

Functional coupling of renal K+ and Na+ handling causes high blood pressure in Na+ replete mice.

Vitzthum, H. et al. (2014) J Physiol. 592(Pt 5):1139-57.

PubMed ID: 24396058 Reactivity Mouse Applications: Western Blot

Double Knockout of Carbonic Anhydrase II (CAII) and Na+-Cl- Cotransporter (NCC) Causes Salt Wasting and Volume Depletion.

Xu, J., Barone, S., Brooks, M.B., Soleimani, M. (2013) Cell Physiol Biochem. 32(suppl 1):173-183.

PubMed ID: 24429824 Reactivity Mouse Applications: Western Blot

Adenylyl Cyclase VI Mediates Vasopressin-Stimulated ENaC Activity.

Roos, K.P. et al. (2012) JASN. 24 (2): 218-227.

PubMed ID: 23264685 Reactivity Mouse Applications: Western Blot

Regional differences in rat conjunctival ion transport activities.

Yu, D. et al. (2012) Am J Physiol Cell Physiol. 303 (7): 767-780.

PubMed ID: 22814399 Reactivity Xenopus laevis Applications: Western Blot

The Epithelial Sodium Channel (ENaC) Establishes a Trafficking Vesicle Pool Responsible for Its Regulation.

Edinger, R.S. et al. (2012) PLoS ONE. 7(9): e46593.

PubMed ID: 23029554 Reactivity Mouse Applications: Western Blot

Double Knockout of pendrein and NaCl cotransporter (NCC) causes severe salt wasting, volume depletion and renal failure.

Soleimani, M. et al. (2012) Proc Natl Acad Sci U S A. 109 (33): 13368-13373.

PubMed ID: 22847418 Reactivity Mouse Applications: Western Blot

Aldosterone does not require angiotensin II to activate NCC through a WNK4–SPAK–dependent pathway.

van der Lubbe, N. et al. (2012) Euro J Physiol. 463 (6): 853-863.

PubMed ID: 22549242 Reactivity Rat Applications: Western Blot

Cortical actin binding protein cortactin mediates ENaC activity via Arp2/3 complex.

Ilatovskaya, D.V., Pavlov, T.S., Levchenko, V., Negulyaev, Y.A. and Staruschenko, A. (2011) FASEB J. 25 (8): 2688-2699.

PubMed ID: 21536685 Reactivity Hamster Applications: Western Blot

Cortical actin binding protein cortactin mediates ENaC activity via Arp2/3 complex.

Ilatovskaya, D.V., Pavlov, T.S., Levchenko, V., Negulyaev, Y.A. and Staruschenko, A. (2011) FASEB J. 25 (8): 2688-2699.

PubMed ID: 21536685 Reactivity Mouse Applications: Western Blot

Immunohistochemistry

Epoxyeicosatrienoic Acid (EET) Analog Lowers Blood Pressure Through Vasodilation And Sodium Channel Inhibition.

Khan, M. A., et al. (2014) Clin Sci (Lond). 127(7):463-74.

PubMed ID: 24707975 Reactivity Rat Applications: Immunohistochemistry

Novel Mechanisms of Sodium Retention in Obesity: Phosphorylation of NKCC2 and Regulation of SPAK/OSR1 by AMPK.

Davies, M. R. P. et al. (2014) Am J Physiol. 307(1):F96-F106.

PubMed ID: 24808538 Reactivity Mouse Applications: Immunohistochemistry

ENaC γ-expressing astrocytes in the circumventricular organs, white matter, and ventral medullary surface: sites for Na+ regulation by glial cells.

Miller, R.L. and Loewy, A.D. (2013) J Chem Neuroanat. 53:72-80.

PubMed ID: 24145067 Reactivity Rat Applications: Immunohistochemistry

Regulation of ENaC in mice lacking renal insulin receptors in the collecting duct.

Pavlov, T.S. et. al. (2013) FASEB J. 27(7):2723-32.

PubMed ID: 23558339 Reactivity Mouse Applications: Immunohistochemistry

 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

 ATTO 700 Fluorophore Absorption and Emission SpectrumOptical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。