STRESTMARQ/抗ENaCα抗体/SPC-403D/100-微克-免疫在线蚂蚁淘旗下平台

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商品详细STRESTMARQ/抗ENaCα抗体/SPC-403D/100-微克

STRESTMARQ/抗ENaCα抗体/SPC-403D/100-微克

商品编号： SPC-403D

品牌： StressMarq Biosciences

市场价： ¥6080.00

美元价： 3648.00

产地：美国(厂家直采)

公司：

产品分类：上样缓冲液

公司分类： DNA_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Overview:

ProductName ENaCAlphaAntibody

Description

RabbitAnti-RatENaCAlphaPolyclonal

SpeciesReactivity Mouse,Rat,Africanclawedfrog(Xenopuslaevis)

Applications WB,IHC,ICC/IF

AntibodyDilution WB(1:1000),ICC/IF(1:400);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Rabbit

ImmunogenSpecies Rat

Immunogen ProducedagainstasyntheticpeptidemappingtotheN-terminalofthealphasubunit(aminoacids46-68)ofratAlphaENaC(antibodydesignation3560-2).

Concentration 1mg/ml

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-ProteinComplex
Smallphycobiliprotein
Isolatedfromredalgae
Largestokesshift(195nm)
MolecularWeight:35kDa

PerCPDatasheet

PerCP Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =482nm

λ_em=677nm

ε_max=1.96x10⁶

Laser =488nm

PE/ATTO594

PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.

Overview:

Highfluorescenceyield
HighphotostABIlity
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation

PE/ATTO594Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

OpticalProperties:

λ_ex =535nm

λ_em =627 nm

Laser = 488to561nm

FITC(Fluorescein)

Overview:

Excellentfluorescencequantumyield
Highrateofphotobleaching
Goodsolubilityinwater
Broademissionspectrum
pHdependentspectra
Molecularformula:C₂₀H₁₂O₅
Molarmass:332.3g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =494 nm

λ_em=520 nm

ε_max=7.3×10⁴

Φ_f= 0.92

τ_fl =5.0ns

Brightness= 67.2

Laser = 488 nm

Filterset = FITC

ATTO700

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:575g/mol

ATTO700Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =700 nm

λ_em=719nm

ε_max=1.25×10⁵

Φ_f= 0.25

τ_fl =1.6ns

Brightness= 31.3

Laser =676nm

Filterset =Cy®5.5

ATTO680

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:631g/mol

ATTO680Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =680nm

λ_em=700 nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.7ns

Brightness=37.5

Laser =633 –676nm

Filterset =Cy®5.5

ATTO655

Overview:

Highfluorescenceyield
Highthermalandphotostability
Excellentozoneresistance
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:634g/mol

ATTO655Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =663nm

λ_em=684nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.8ns

Brightness= 37.5

Laser =633 –647nm

Filterset =Cy®5

ATTO633

Overview:

Highfluorescenceyield
Highthermalandphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
StableatpH4–11
Cationicdye,perchloratesalt
MolarMass:652.2g/mol

ATTO633Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =629nm

λ_em=657nm

ε_max=1.3×10⁵

Φ_f= 0.64

τ_fl =3.2ns

Brightness=83.2

Laser =633 nm

Filterset =Cy®5

ATTO594

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:1137g/mol

ATTO594Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

OpticalProperties:

λ_ex =601 nm

λ_em=627nm

ε_max=1.2×10⁵

Φ_f= 0.85

τ_fl =3.5ns

Brightness=102

Laser =594nm

Filterset =TexasRed®

ATTO565

Overview:

Highfluorescenceyield
Highthermalandphotostability
Goodsolubilityinpolarsolvents
Excellentsolubilityinwater
Verylittleaggregation
Rhodaminedyederivative
MolarMass:611g/mol

ATTO565Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =563nm

λ_em=592nm

ε_max=1.2×10⁵

Φ_f= 0.9

τ_fl =3.4n

Brightness=10

Laser =532nm

Filterset = TRITC

ATTO488

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:804g/mol

ATTO488Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =501nm

λ_em=523nm

ε_max=9.0×10⁴

Φ_f= 0.80

τ_fl =4.1ns

Brightness= 72

Laser = 488 nm

Filterset = FITC

ATTO390

Overview:

Highfluorescenceyield
LargeStokes-shift(89nm)
Goodphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
Coumarinderivate,uncharged
Lowmolarmass:343.42g/mol

ATTO390Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

OpticalProperties:

λ_ex =390nm

λ_em=479nm

ε_max=2.4×10⁴

Φ_f= 0.90

τ_fl =5.0ns

Brightness= 21.6

Laser =365or405nm

APC(Allophycocyanin)

Overview:

Highquantumyield
Largephycobiliprotein
6chromophorespermolecule
Isolatedfromredalgae
MolecularWeight:105kDa

APCDatasheet

APC Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =650nm

λ_em=660nm

ε_max=7.0×10⁵

Φ_f= 0.68

Brightness= 476

Laser =594or633 nm

Filterset =Cy®5

Streptavidin

Properties:

Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinity
Molecularweight:53kDa
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

StreptavidinDatasheet

Biotin

Properties:

BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity
Molarmass:244.31g/mol
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

BiotinDatasheet

HRP(HorserADIshperoxidase)

Properties:

Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
Readilycombineswithhydrogenperoxide(H₂O₂)toformHRP-H₂O₂complexwhichcanoxidizevarioushydrogendonors
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
Highturnoverrateenablesrapidgenerationofastrongsignal
44kDaglycoprotein
Extinctioncoefficient:100(403nm)
Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

AP(AlkalinePhosphatase)

Properties:

Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols
Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts
- Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts
Molecularweight:140kDa
Applications:Westernblot,immunohistochemistry,andELISA

APDatasheet

R-PE(R-Phycoerythrin)

Overview:

Broadexcitationspectrum
Highquantumyield
Photostable
Memberofthephycobiliproteinfamily
Isolatedfromredalgae
Excellentsolubilityinwater
MolecularWeight:250kDa

R-PEDatasheet

R-PE Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =565nm

λ_em=575nm

ε_max=2.0×10⁶

Φ_f= 0.84

Brightness=1.68x10³

Laser = 488to561nm

Filterset = TRITC

Properties

StorageBuffer	PBS,50%glycerol,0.09%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	ProteinApurified
Clonality	Polyclonal
Specificity	Detects~85kDa.
CiteThisProduct	StressMarqBiosciencesCat#SPC-403,RRID:AB_10640131
CertificateofAnalysis	1µg/mlofSPC-403wassufficientfordetectionofalpha-ENaCin35µgofratkidneytissuelysatebycolorimetricimmunoblotanalysisusingGoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	SCNN1AAntibody,EpithelialSodiumChannel-αAntibody,EpithelialSodiumChannelalphaAntibody,AlphaENaC2Antibody,AlphaENaCAntibody,AlphaNaCHAntibody,Alpha-ENaCAntibody,AmiloridesensitiveepithelialsodiumchannelalphasubunitAntibody,AmiloridesensitivesodiumchannelsubunitalphaAntibody,Amiloride-sensitivesodiumchannelsubunitalphaAntibody,ENaCaAntibody,ENaCalphaAntibody,EpithelialNa(+)channelsubunitalphaAntibody,EpithelialNa+channelsubunitalphaAntibody,FLJ21883Antibody,Nonvoltagegatedsodiumchannel1subunitalphaAntibody,Nonvoltage-gatedsodiumchannel1subunitalphaAntibody,SCNEAAntibody,SCNN1Antibody,SCNN1Antibody,SCNN1AAntibody,SCNNA_HUMANAntibody,Sodiumchannelnonvoltagegated1alphaAntibody
ResearchAreas	EpithelialSodiumChannels(ENaC),IonChannels,Neuroscience,SodiumChannels
CellularLocalization	Apicalcellmembrane
AccessionNumber	NP_113736
GeneID	25122
SwissProt	Q6IRJ1
ScientificBackground	TheEpithelialSodiumChannel(ENaC)isamembraneionchannelpermeabletoNa+ions.Itislocatedintheapicalplasmamembraneofepitheliainthekidneys,lung,colon,andothertissueswhereitplaysaroleintransepithelialNa+-iontransport(1).SpecificallyNa+transportviaENaCoccursacrossmanyepithelialsurfaces,andplaysakeyroleinregulatingsaltandwaterabsorption(2).ENaCsarecomposedofthreestructurallyrelatedsubunitsthatformatetramericchannel,α,β,andγ.Theexpressionofitsalphaandbetasubunitsisenhancedaskeratinocytesdifferentiate(3,4).Thebetaandgamma-ENaCsubunitsareessentialforedemafluidtoexertitsmaximaleffectonnetfluidabsorptionbydistallungepithelia(5).Andithasbeenconcludedthatthesubunitsaredifferentiallyexpressedintheretinaofmicewithocularhypertension,thereforetheup-regulationofalpha-ENaCproteinscouldserveasaprotectionmechanismagainstelevatedintraocularpressure(6).
References	1.KakizoeY.,etal.(2009)JHpyertens.27(8):1679-1689. 2.GuY.(2008)JCellPhysiol.216(2):453-457. 3.BrunsJ.B.(2003)AmJPhysiolRenalPhysiol.285(4):F600-F609. 4.MauroT.,etal.(2002)JInvestDermatol.118(4):589-594. 5.EliasN.,etal.(2007)AmJPhysiolLungCellMolPhysiol.293(3):L537-45. 6.DykaF.M.,MayC.A.andEnzR.(2005)JNeuRochem.94(1):120-128.

ProductImages

<p>Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-403). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-403) at 1:1000.</p>

WesternblotanalysisofRatkidneytissuelysatesshowingdetectionofENaCproteinusingRabbitAnti-ENaCPolyclonalAntibody(SPC-403).PrimaryAntibody:RabbitAnti-ENaCPolyclonalAntibody(SPC-403)at1:1000.

<p>Western blot analysis of Mouse kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-403). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-403) at 1:1000.</p>

WesternblotanalysisofMousekidneytissuelysatesshowingdetectionofENaCproteinusingRabbitAnti-ENaCPolyclonalAntibody(SPC-403).PrimaryAntibody:RabbitAnti-ENaCPolyclonalAntibody(SPC-403)at1:1000.

ProductCitations(34)

WesternBlot

EnacRegulationintheKidney:theRoleofAnkyrinG

Klemens,C.A.(2017)UniversityofPittsburgh.PhDDissertation.

PubMedID:N/AReactivityRatApplications:WesternBlot

Derlin-1promotesubiquitinationanddegradationofepithelialsodiumchannel(ENaC).

You,H.etal.(2017)JCellSci.130(6):1027-1036.

PubMedID:28137758ReactivityMouseApplications:WesternBlot

Derlin-1promotesubiquitinationanddegradationofepithelialsodiumchannel(ENaC).

You,H.etal.(2017)JCellSci.130(6):1027-1036.

PubMedID:28137758ReactivityHumanApplications:WesternBlot

AnkyrinGExpressionRegulatesApicalDeliveryoftheEpithelialSodiumChannel(ENaC).

Klemens,C.A.,Edinger,R.S.,Kightlinger,L.,Liu,X.andButterworth,M.B.(2016)JBiolChem.292(1):375-385.

PubMedID:27895120ReactivityMouseApplications:WesternBlot

Renalsodiumtransportinrenin-deficientDahlsalt-sensitiverats.

Pavlov,T.S.,Levchenko,V.,Ilatovskaya,D.V.,Moreno,C.andStaruschenko,A.(2016)JReninAngiotensinAldosteroneSyst.17(3).

PubMedID:27443990ReactivityRatApplications:WesternBlot

(Pro)ReninReceptorRegulatesPotassiumHomeostasisthroughaLocalMechanism.

Xu,C.etal.(2016)AmJPhysiolRenalPhysiol.[Epubaheadofprint]

PubMedID:27440776ReactivityRatApplications:WesternBlot

AMP-activatedproteinkinaseattenuateshighsalt-inducedactivationofepithelialsodiumchannels(ENaC)inhumanumbilicalveinendothelialcells.

Zheng,W.etal.(2016)OxidMedCellLongev.2016:1531392.

PubMedID:27635187ReactivityHumanApplications:WesternBlot

Renaltubularepithelialcellproreninreceptorregulatesbloodpressureandsodiumtransport.

Ramkumar,N.etal.(2016)AmJPhysiolRenalPhysiol.311(1):F186-94.

PubMedID:27053687ReactivityMouseApplications:WesternBlot

TheRoleofEpithelialSodiumChannelENaCandtheApicalCl-/HCO3-ExchangerPendrininCompensatorySaltReabsorptionintheSettingofNa-ClCotransporter(NCC)Inactivation.

Patel-Chamberlin,M.etal.(2016)PLoSOne.11(3):e0150918.

PubMedID:26963391ReactivityMouseApplications:WesternBlot

PossIBLerolefornephron-derivedangiotensinogeninangiotensin-IIdependenthypertension.

Ramkumar,N.etal.(2016)PhysiolRep.4(1).pii:e12675.

PubMedID:26755736ReactivityMouseApplications:WesternBlot

TheThiazide-SensitiveCo-TransporterPromotestheDevelopmentofSodiumRetentioninMicewithDiet-InducedObesity.

Davies,M.R.P.etal.(2015)KidneyBloodPressRes.40(5):509-519.

PubMedID:26418861ReactivityMouseApplications:WesternBlot

InhibitionofMitochondrialComplex-1PreventstheDownregulationofNKCC2andENaCαinObstructiveKidneyDisease.

Zhang,Y.etal.(2015)SciRep.5:12480.

PubMedID:26207612ReactivityMouseApplications:WesternBlot

IncreasedEpithelialSodiumChannelActivityContributestoHypertensionCausedbyNa+-HCO3−CotransporterElectrogenic2Deficiency.

Wen,D.etal.(2015)Hypertension.66(1):68-74.

PubMedID:25941340ReactivityMouseApplications:WesternBlot

CompositionsAndMethodsOfUseFor(PRO)ReninReceptor.

Feng,Y.andYang,T.(2015)UnitedStatesPatentApplication20150025013.

PubMedID:N/AReactivityRatApplications:WesternBlot

RoleofRhoGDP-dissociationinhibitoralphaincontrolofENaC-mediatedsodiumreabsorption.

Pavlov,T.S.,Levchenko,V.andStaruschenko,A.(2014)JBiolChem.289(41):28651-9.

PubMedID:25164814ReactivityMouseApplications:WesternBlot

Collectingductspecificknock-outofreninattenuatesangiotensin-IIinducedhypertension.

Ramkumar,N.etal.(2014)AmJPhysiolRenalPhysiol.307(8):F931-8.

PubMedID:25122048ReactivityMouseApplications:WesternBlot

NovelMechanismsofSodiumRetentioninObesity:PhosphorylationofNKCC2andRegulationofSPAK/OSR1byAMPK.

Davies,M.R.P.etal.(2014)AmJPhysiol.307(1):F96-F106.

PubMedID:24808538ReactivityMouseApplications:WesternBlot

ChronicAngiotensinIIInfusionDrivesExtensiveAldosterone-IndependentEpithelialNa+ChannelActivation.

Mamenko,M.et.al.(2013)Hypertension.62(6):1111-22.

PubMedID:24060890ReactivityMouseApplications:WesternBlot

AdenylylCyclaseVIMediatesVasopressin-StimulatedENaCActivity.

Roos,K.P.etal.(2012)JASN.24(2):218-227.

PubMedID:23264685ReactivityMouseApplications:WesternBlot

IncreasedexpressionofrenalTRPM6compensatesforMg2+wastingduringfurosemidetreatment.

vanAngelen,A.A.,Kemp,A.W.,Hoenderop,J.G.andBindels,B.J.(2012)ClinKidneyJ.5(6):535-544.

PubMedID:26069797ReactivityMouseApplications:WesternBlot

Regionaldifferencesinratconjunctivaliontransportactivities.

Yu,D.etal.(2012)AmJPhysiolCellPhysiol.303(7):767-780.

PubMedID:22814399ReactivityXenopuslaevisApplications:WesternBlot

TheEpithelialSodiumChannel(ENaC)EstablishesaTraffickingVesiclePoolResponsibleforItsRegulation.

Edinger,R.S.etal.(2012)PLoSONE.7(9):e46593.

PubMedID:23029554ReactivityMouseApplications:WesternBlot

AldosteronedoesnotrequireangiotensinIItoactivateNCCthroughaWNK4–SPAK–dependentpathway.

vanderLubbe,N.etal.(2012)EuroJPhysiol.463(6):853-863.

PubMedID:22549242ReactivityRatApplications:WesternBlot

Immunohistochemistry

TheProstaglandinE2Receptor1(EP1)AntagonizesAngIIintheCollectingDuct.

Eckert,D.(2017)UniversityofOttawa.Dissertation.

PubMedID:N/AReactivityMouseApplications:Immunohistochemistry

InhibitionofMitochondrialComplex-1PreventstheDownregulationofNKCC2andENaCαinObstructiveKidneyDisease.

Zhang,Y.etal.(2015)SciRep.5:12480.

PubMedID:26207612ReactivityMouseApplications:Immunohistochemistry

EpoxyeicosatrienoicAcid(EET)AnalogLowersBloodPressureThroughVasodilationAndSodiumChannelInhibition.

Khan,M.A.,etal.(2014)ClinSci(Lond).127(7):463-74.

PubMedID:24707975ReactivityRatApplications:Immunohistochemistry

ChronicAngiotensinIIInfusionDrivesExtensiveAldosterone-IndependentEpithelialNa+ChannelActivation.

Mamenko,M.et.al.(2013)Hypertension.62(6):1111-22.

PubMedID:24060890ReactivityMouseApplications:Immunohistochemistry

ENaCγ-expressingastrocytesinthecircumventricularorgans,whitematter,andventralmedullarysurface:sitesforNa+regulationbyglialcells.

Miller,R.L.andLoewy,A.D.(2013)JChemNeuroanat.53:72-80.

PubMedID:24145067ReactivityRatApplications:Immunohistochemistry

RegulationofENaCinmicelackingrenalinsulinreceptorsinthecollectingduct.

Pavlov,T.S.et.al.(2013)FASEBJ.27(7):2723-32.

PubMedID:23558339ReactivityMouseApplications:Immunohistochemistry

OtherCitations

Derlin-1promotesubiquitinationanddegradationofepithelialsodiumchannel(ENaC).

You,H.etal.(2017)JCellSci.130(6):1027-1036.

PubMedID:28137758ReactivityMouseApplications:Immunoprecipitation

Derlin-1promotesubiquitinationanddegradationofepithelialsodiumchannel(ENaC).

You,H.etal.(2017)JCellSci.130(6):1027-1036.

PubMedID:28137758ReactivityHumanApplications:Immunoprecipitation

ENaCandROMKactivityareinhibitedintheDCT2/CNTofTgWnk4PHAIImice.

Zhang,C.etal.(2016)AmJPhysiolRenalPhysiol.[Epubaheadofprint]

PubMedID:N/A

mTORC1maintainsrenaltubularhomeostasisandisessentialinresponsetoischemicstress.

Grahammer,F.etal.(2014)ProcNatlAcadSciUSA.111(27):E2817-26.

PubMedID:24958889ReactivityMouseApplications:GelShift

mPGES-1-DerivedPGE2MediatesDehydrationNatriuresis.

Jia,Z.etal.(2013)AmJPhysiolRenalPhysiol.304(2):F214-21.

PubMedID:23171554ReactivityMouseApplications:WesternBlot

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