STRESS MARQ/STRESS XPRESS®循环放大器EIA套件/SKT-209-96/96井
商品编号：
SKT-209-96
市场价：
¥7700.00
美元价：
4620.00
产品分类：
电泳缓冲液
公司分类：
Electrophoretic_buffer
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | CyclicAMPEIAKit |
| Description | QuantitativecolorimetricdetectionofcAMP |
| SpeciesReactivity | SpeciesIndependent |
| Platform | Microplate |
| SampleTypes | Celllysates,EDTAPlasma,HeparinPlasma,Saliva,Tissue,TissueCultureMedia,Urine |
| DetectionMethod | ColorimetricAssay |
| AssayType | DirectEIA(EnzymeImmunoassay) |
| Utility | EIAkitusedtoquantitativelymeasurecAMPpresentinsamples. |
| Sensitivity | 0.64pmol/ml |
| AssayRange | Regular:0.617-150pmol/ml.Acetylated:0.156-10pmol/ml |
| Precision | IntraAssayPrecision(Regular):ThreehumanurinesamplesweredilutedwithSampleDiluentandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedcAMPconcentrationswere:Sample1-56.9pmol/mL,8.6%CVSample2-11.9pmol/mL,11.3%CVSample3-6.7pmol/mL,12.3%CVIntraAssayPrecision(Acetylated):TwohumanplasmasamplesweredilutedwithSampleDiluent,acetylatedandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedcAMPconcentrationswere:Sample1-1.74pmol/mL,10.4%CVSample2-0.60pmol/mL,11.8%CVInterAssayPrecision(Regular):ThreehumanurinesamplesweredilutedwithSampleDiluentandruninduplicatesintwelveassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedcAMPconcentrationswere:Sample1-57.1pmol/mL,10.0%CVSample2-10.9pmol/mL,11.5%CVSample3-6.3pmol/mL,11.3%CVInterAssayPrecision(Acetylated):OnehumanurineandtwohumanplasmasampleweredilutedwithSampleDiluent,acetylatedandruninduplicatesintwelveassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedcAMPconcentrationswere:Sample1-5.22pmol/mL,9.8%CVSample2-0.99pmol/mL,8.1%CVSample3-0.73pmol/mL,15.4%CV |
| IncubationTime | 2.5Hours |
| NumberofSamples | 39samplesinduplicate |
| OtherResources | KitBooklet,MSDS,SalivaSampleHandlingInstructions |
Properties
| StorageTemperature | 4ºC | |||||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | |||||||||||||||||||||||||||||||||||||||
| ProductType | EIAKits | |||||||||||||||||||||||||||||||||||||||
| AssayOverview | TheCyclicAMPEIAkitisdesignedtoquantitativelymeasurecAMPpresentinlysedcells,EDTAandheparinplasma,urine,salivaandtissueculturemediasamples.Fortissuesamples,salivaandurine,wherethelevelsofcAMPareexpectedtoberelativelyhigh,theregularformatfortheassaycanbeused.Forplasmasamplesandsomedilutecelllysatesanoptionalacetylationprotocolcanbeused.ThekitisuniqueinthatallsamplesandstandardsaredilutedintoanacidicSampleDiluent,whichcontainsspecialadditivesandstABIlizers,forcAMPmeasurement.Thisallowsplasma,urineandsalivasamplestobereadinanidenticalmannertolysedcells.AcidifiedsamplesofcAMParestableandendogenousphosphodiesterasesareinactivatedintheSampleDiluent.AcAMPstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.AclearmicrotiterplatecoatedwithanantibodytocapturesheepIgGisprovidedandaneutralizingPlatePrimersolutionisaddedtoalltheusedwells.Standardsordilutedsamples,eitherwithorwithoutacetylation,arePipettedintotheprimedwells.AcAMP-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.ThebindingreactionisinitiatedbytheadditionofasheepantibodytocAMPtoeachwell.Aftera2hourincubation,theplateiswashedandsubstrateisadded.ThesubstratereactswiththeboundcAMP-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.TheconcentrationofthecAMPinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders. | |||||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | CyclicAMPEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-209) |
BIOLOGicalDescription
| AlternativeNames | 3"-5"-cyclicadenosinemonophosphateEIAKit |
| ResearchAreas | CellSignaling,Neuroscience |
| ScientificBackground | Adenosine-3’,5’-cyclicmonophosphate(cyclicAMP)isasecondmessengerthatplaysanimportantroleinintracellularregulation.Specifically,itfunctionsasamediatorofactivityofseveralkeyhormonesincludingepinephrine,glucagon,andACTH(1-4).AdenylatecyclaseisactivatedbythehormonesglucagonandadrenalineandbyGprotein.Liveradenylatecyclaserespondsmorestronglytoglucagon,andmuscleadenylatecyclaserespondsmorestronglytoadrenaline.cAMPdecompositionintoAMPiscatalyzedbytheenzymephosphodiesterase.IntheHumanMetabolomeDatabasethereare166metabolicenzymeslistedthatconvertcAMP(5).OtherbiologicalactionsofcAMPincluderegulationofinnateimmunefunctioning(6),axonregeneration(7),cancer(8),andinflammation(9). |
| References | 1.Sutherland,E.W.andRall,T.W.FractionationandCharacterizationofaCyclicAdenineRibonucleotideFormedbyTissueParticles.J.Biol.Chem.,232:1077,1958. 2.Marsh,J.M.Biol.Reprod.,14:30-53,1976. 3.Korenman,S.G.andKrall,J.F.Biol.Reprod.,16:1-17,1977. 4.Kelley,D.J.,Bhattacharyya,A.,Lahvis,G.P.,Yin,J.C.P.,Malter,J.,andDavidson,R.J.Neurosci.Biobehav.Rev.,32(8):1533-1543,2008. 5.http://www.hmdb.ca/metabolites/HMDB00058 6.Serezani,C.H.,Ballinger,M.N.,Aronoff,D.M.,andPeters-Golden,M.Am.J.Resp.CellandMol.Biol.,39(2):127,2008. 7.Hannila,S.S.,andFilbin,M.T.Exp.Neurol.,209(2):321–332,2008. 8.Shankar,D.B,Cheng,J.C.,andSakamoto,K.M.Cancer,104(9):1819-24,2005. 9.GaleaE.andFeinstein,D.L.FASEBJ.,13:2125-2137,1999. |
ProductImages
RegularFormatTypicalStandardCurvefortheCyclicAMPEIAKit(EnzymeImmunoassay)StressXpress®–SKT-209.AssayType:DirectEIA.DetectionMethod:ColorimetricAssay.AssayRange:Regular:0.617–150pmol/ml.Acetylated:0.156–10pmol/ml.
AcetylatedFormatTypicalStandardCurvefortheCyclicAMPEIAKit(EnzymeImmunoassay)StressXpress®–SKT-209.AssayType:DirectEIA.DetectionMethod:ColorimetricAssay.AssayRange:Regular:0.617–150pmol/ml.Acetylated:0.156–10pmol/ml.
ChemicalstructureofCyclicAMP(Adenosine-3,5-cyclicmonophosphate)
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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
