StressMarq/Anti-Cox 2 Antibody/SPC-1321D-A565/100-µl-免疫在线蚂蚁淘旗下平台

当前位置：首页 > 产品中心 > Electrophoretic_buffer > StressMarq/Anti-Cox 2 Antibody/SPC-1321D-A565/100-µl

商品详细StressMarq/Anti-Cox 2 Antibody/SPC-1321D-A565/100-µl

StressMarq/Anti-Cox 2 Antibody/SPC-1321D-A565/100-µl

商品编号： SPC-1321D-A565

品牌： StressMarq Biosciences

市场价： ¥6040.00

美元价： 3624.00

产地：美国(厂家直采)

公司：

产品分类：电泳缓冲液

公司分类： Electrophoretic_buffer

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Overview:

Product Name Cox 2 Antibody

Description

Rabbit Anti-Human Cox 2 Polyclonal

Species Reactivity Human, Mouse, Rat

Applications WB, IHC, ICC/IF, ELISA

Antibody Dilution WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.

Host Species Rabbit

Immunogen Species Human

Immunogen Synthesized peptide derived from human Cox2.

Conjugates

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Affinity Purified
Clonality	Polyclonal
Isotype	IgG
Specificity	Detects endogenous levels of total Cox2 protein.
Cite This Product	StressMarq Biosciences Cat# SPC-1321, RRID: AB_2713565
Certificate of Analysis	A 1:1000 dilution of SPC-1321 was sufficient for detection of Cox2 in 10 µg of A549 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names	COX 2 antibody, COX3 antibody, Cyclooxygenase 1 antibody, PCOX 1 antibody, PGG/HS antibody, PGH synthase 2 antibody, PGHS 1 antibody, PHS II antibody, Prostaglandin endoperoxide synthase 1 antibody, Prostaglandin endoperoxide synthase 2 antibody, PTGS2 antibody, TIS10 antibody
Cellular Localization	Endoplasmic reticulum membrane, Microsome Membrane
Accession Number	NP_000954.1
Gene ID	5743
Swiss Prot	P35354
Scientific Background	Cyclooxygenase (COX), also known as Prostaglandin H2 synthase and Prostaglandin endoperoxide synthase, is a key enzyme in the of conversion arachidonic acid to Prostaglandin H2. Prostaglandin H2 is converted by other enzymes into inflammatory mediators prostaglandin (PG) D2, PGE2, PGF2a, PGI2 and Thromboxane A2. Thus, COX is a key enzyme in the production of inflammatory agents and is the target of intense research and drug discovery activities. There are two enzymes of COX, COX 1 and COX 2.COX 2 is found in elevated levels in synoviocytes from rheumatoid arthritis patients. The discomforts of inflammation such as pain and swelling are largely due to the action of prostaglandins produced by COX-2. COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.
References	1. Jones D.A. et al. (1993) J. Biol. Chem. 268:9049-9054. 2. Kosaka T. et al. (1994) Eur. J. Biochem. 221:889-897. 3. Appleby S.B. et al. (1994) Biochem. J. 302:723-727. 4. Barnett J. et al. (1994) Biochim. Biophys. Acta 1209:130-139.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321). Tissue: A549. Species: Human. Primary Antibody: Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321). Tissue: A549. Species: Human. Primary Antibody: Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Western blot analysis of Human A549 cell lysates showing detection of ~74kDa Cox2 protein using Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321). Lane 1: Human A549. Lane 2: Human A549 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321) at 1:1000. Predicted/Observed Size: ~74kDa.</p>

Western blot analysis of Human A549 cell lysates showing detection of ~74kDa Cox2 protein using Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321). Lane 1: Human A549. Lane 2: Human A549 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321) at 1:1000. Predicted/Observed Size: ~74kDa.

Product Citations (0)

Currently there are no citations for this product.

ATTO 565

Overview:

High fluorescence yield
High thermal and photostability
Good solubility in polar solvents
Excellent solubility in water
Very little aggregation
Rhodamine dye derivative
Molar Mass: 611 g/mol

ATTO 565 Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 563 nm

λ_em = 592 nm

ε_max = 1.2×10⁵

Φ_f = 0.9

τ_fl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

公司介绍