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当前位置： 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/Anti-Collagen IV Antibody/SPC-1276D-A488/100-µl
商品详细StressMarq/Anti-Collagen IV Antibody/SPC-1276D-A488/100-µl
StressMarq/Anti-Collagen IV Antibody/SPC-1276D-A488/100-µl
StressMarq/Anti-Collagen IV Antibody/SPC-1276D-A488/100-µl
商品编号： SPC-1276D-A488
市场价： ¥6040.00
美元价： 3624.00
产地： 美国(厂家直采)
公司：
产品分类： 电泳缓冲液
公司分类： Electrophoretic_buffer
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name Collagen IV Antibody
Description

Rabbit Anti-Human Collagen IV Polyclonal

Species Reactivity Human, Mouse
Applications WB, IHC, ELISA
Antibody Dilution WB (1:1000), IHC (1:100), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthesized peptide derived from human Collagen IV.
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Isotype IgG
Specificity Detects endogenous levels of total Collagen IV protein.
Cite This Product StressMarq Biosciences Cat# SPC-1276, RRID: AB_2712773
Certificate of Analysis A 1:1000 dilution of SPC-1276 was sufficient for detection of Collagen IV in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Arresten Antibody, CO4A1_HUMAN Antibody, collagen alpha-1(IV) chain Antibody, Collagen Of Basement Membrane Alpha 1 Chain Antibody, Collagen Type IV Antibody
Research Areas Cancer, Cell Signaling, Chondrogenesis, Collagen, Extracellular Matrix (ECM), Invasion/microenvironment, Mesenchymal Stem Cells, Stem Cells
Cellular Localization Extracellular Matrix
Accession Number NP_001836.3
Gene ID 1282
Swiss Prot P02462
Scientific Background Type-IV collagen is found primarily in the basal lamina. It has been associated with Goodpasture's Disease, as well as Alport Syndrome.
References 1. Turner N., et al. (1992) J Clin Invest. 89(2): 592–601.
2. "Entrez Gene: COL4A3 collagen, type IV, alpha 3 (Goodpasture antigen)".

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Western blot analysis of Human HeLa cell lysates showing detection of ~160kDa Collagen IV protein using Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276). Lane 1: Human HeLa cells. Lane 2: Human HeLa cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276) at 1:1000. Predicted/Observed Size: ~160kDa.</p>

Western blot analysis of Human HeLa cell lysates showing detection of ~160kDa Collagen IV protein using Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276). Lane 1: Human HeLa cells. Lane 2: Human HeLa cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276) at 1:1000. Predicted/Observed Size: ~160kDa.

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  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

  ATTO 488 Fluorophore Excitation and Emission SpectraOptical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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