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当前位置： 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/Anti-Collagen II Antibody/SPC-1274D-A594/100-µl
商品详细StressMarq/Anti-Collagen II Antibody/SPC-1274D-A594/100-µl
StressMarq/Anti-Collagen II Antibody/SPC-1274D-A594/100-µl
StressMarq/Anti-Collagen II Antibody/SPC-1274D-A594/100-µl
商品编号： SPC-1274D-A594
市场价： ¥6040.00
美元价： 3624.00
产地： 美国(厂家直采)
公司：
产品分类： 电泳缓冲液
公司分类： Electrophoretic_buffer
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name Collagen II Antibody
Description

Rabbit Anti-Human Collagen II Polyclonal

Species Reactivity Human, Mouse, Rat
Applications WB, IHC, ICC/IF, ELISA
Antibody Dilution WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthesized peptide derived from human Collagen II.
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Isotype IgG
Specificity Detects endogenous levels of total Collagen II protein.
Cite This Product StressMarq Biosciences Cat# SPC-1274, RRID: AB_2712737
Certificate of Analysis A 1:1000 dilution of SPC-1274 was sufficient for detection of Collagen II in 10 µg of COLO205 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Alpha 1 type II collagen Antibody, AOM Antibody, Cartilage collagen Antibody, Chondrocalcin Antibody, COL11A3 Antibody, Collagen II alpha 1 polypeptide Antibody, SEDC Antibody
Research Areas Bone, Cell Signaling, Collagen, Extracellular Matrix (ECM), Mesenchymal Stem Cells, Osteogenesis, Stem Cells
Cellular Localization Extracellular Matrix
Accession Number NP_149162.2
Gene ID 1280
Swiss Prot P02458
Scientific Background Type-II collagen is the less abundant form of collagen in the human body and can be found in articular and hyaline cartilages and the eyes. It has been associated with the treatment of arthritis, cellulite and wrinkles.
References 1. Mullazehi M., et al. (2012) Arthritis Research Ther. 14:R100

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Tissue: Cos7. Species: Monkey. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Tissue: Cos7. Species: Monkey. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Western blot analysis of Human COLO205 cell lysates showing detection of ~142kDa Collagen II protein using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Lane 1: Human COLO205. Lane 2: Human COLO205 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:1000. Predicted/Observed Size: ~142kDa.</p>

Western blot analysis of Human COLO205 cell lysates showing detection of ~142kDa Collagen II protein using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Lane 1: Human COLO205. Lane 2: Human COLO205 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:1000. Predicted/Observed Size: ~142kDa.

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 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

 ATTO 594 Fluorophore Excitation and Emission SpectrumOptical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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