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当前位置: 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/抗胶原蛋白II抗体/SPC-1274D-A565/100-µl
商品详细StressMarq/抗胶原蛋白II抗体/SPC-1274D-A565/100-µl
StressMarq/抗胶原蛋白II抗体/SPC-1274D-A565/100-µl
StressMarq/抗胶原蛋白II抗体/SPC-1274D-A565/100-µl
商品编号: SPC-1274D-A565
市场价: ¥6040.00
美元价: 3624.00
产地: 美国(厂家直采)
公司:
产品分类: 电泳缓冲液
公司分类: Electrophoretic_buffer
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameCollagenIIAntibody
Description

RabbitAnti-HumanCollagenIIPolyclonal

SpeciesReactivityHuman,Mouse,Rat
ApplicationsWB,IHC,ICC/IF,ELISA
AntibodyDilutionWB(1:1000),IHC(1:100),ICC/IF(1:500),ELISA(1:20000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenSynthesizedpeptidederivedfromhumanCollagenII.
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityPolyclonal
IsotypeIgG
SpecificityDetectsendogenouslevelsoftotalCollagenIIprotein.
CiteThisProductStressMarqBiosciencesCat#SPC-1274,RRID:AB_2712737
CertificateofAnalysisA1:1000dilutionofSPC-1274wassufficientfordetectionofCollagenIIin10µgofCOLO205celllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesAlpha1typeIIcollagenAntibody,AOMAntibody,CartilagecollagenAntibody,ChondrocalcinAntibody,COL11A3Antibody,CollagenIIalpha1polypeptideAntibody,SEDCAntibody
ResearchAreasBone,CellSignaling,Collagen,ExtracellularMatrix(ECM),MesenchymalStemCells,Osteogenesis,StemCells
CellularLocalizationExtracellularMatrix
AccessionNumberNP_149162.2
GeneID1280
SwissProtP02458
ScientificBackgroundType-IIcollagenisthelessabundantformofcollageninthehumanbodyandcanbefoundinarticularandhyalinecartilagesandtheeyes.Ithasbeenassociatedwiththetreatmentofarthritis,celluliteandwrinkles.
References1.MullazehiM.,etal.(2012)ArthritisResearchTher.14:R100

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:100. The image on the right is treated with the synthesized peptide.</p>

ImmunohistochemistryanalysisusingRabbitAnti-CollagenIIPolyclonalAntibody(SPC-1274).Tissue:BreastCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-CollagenIIPolyclonalAntibody(SPC-1274)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Tissue: Cos7. Species: Monkey. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-CollagenIIPolyclonalAntibody(SPC-1274).Tissue:Cos7.Species:Monkey.PrimaryAntibody:RabbitAnti-CollagenIIPolyclonalAntibody(SPC-1274)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Western blot analysis of Human COLO205 cell lysates showing detection of ~142kDa Collagen II protein using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Lane 1: Human COLO205. Lane 2: Human COLO205 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:1000. Predicted/Observed Size: ~142kDa.</p>

WesternblotanalysisofHumanCOLO205celllysatesshowingdetectionof~142kDaCollagenIIproteinusingRabbitAnti-CollagenIIPolyclonalAntibody(SPC-1274).Lane1:HumanCOLO205.Lane2:HumanCOLO205treatedwiththeimmunizingpeptide.PrimaryAntibody:RabbitAnti-CollagenIIPolyclonalAntibody(SPC-1274)at1:1000.Predicted/ObservedSize:~142kDa.

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 ATTO565
Overview:
  • Highfluorescenceyield
  • HighthermalandphotostABIlity
  • Goodsolubilityinpolarsolvents
  • Excellentsolubilityinwater
  • Verylittleaggregation
  • Rhodaminedyederivative
  • MolarMass:611g/mol

ATTO565Datasheet

 ATTO 565 Fluorophore Excitation and Emission SpectraOpticalProperties:

λex =563nm

λem=592nm

εmax=1.2×105

Φf= 0.9

τfl =3.4n

Brightness=10

Laser =532nm

Filterset = TRITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。