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当前位置: 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/Anti-Collagen I Antibody/SPC-1273D-A488/100-µl
商品详细StressMarq/Anti-Collagen I Antibody/SPC-1273D-A488/100-µl
StressMarq/Anti-Collagen I Antibody/SPC-1273D-A488/100-µl
StressMarq/Anti-Collagen I Antibody/SPC-1273D-A488/100-µl
商品编号: SPC-1273D-A488
市场价: ¥6040.00
美元价: 3624.00
产地: 美国(厂家直采)
公司:
产品分类: 电泳缓冲液
公司分类: Electrophoretic_buffer
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name Collagen I Antibody
Description

Rabbit Anti-Human Collagen I Polyclonal

Species Reactivity Human, Mouse, Rat
Applications IHC, ICC/IF, ELISA
Antibody Dilution IHC (1:100), ICC/IF (1:500), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthesized peptide derived from N-terminal of human Collagen I.
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Isotype IgG
Specificity Detects ~139kDa, endogenous levels of total Collagen I protein. Collage type I is a triple helix consisting of one alpha-2 and 2 alpha-1 chains, so dimeric (~270kDa) and trimeric (~400kDa) forms may
Cite This Product StressMarq Biosciences Cat# SPC-1273, RRID: AB_2712719
Certificate of Analysis A 1:100 dilution of SPC-1273 was sufficient for detection of Collagen I in NIH/3T3 cells using Goat Anti-Rabbit IgG as the secondary antibody.

Biological Description

Alternative Names Alpha 1 type I collagen Antibody, Alpha 2 type I collagen Antibody, CO1A1_HUMAN Antibody, collagen alpha 1 chain type I Antibody, EDSC Antibody, Type I procollagen antibody
Research Areas Cell Signaling, Collagen, Extracellular Matrix (ECM), Mesenchymal Stem Cells, Osteogenesis, Stem Cells
Cellular Localization Extracellular Matrix
Accession Number NP_000080.2
Gene ID 1278
Swiss Prot P08123
Scientific Background Type-I collagen is the predominant form of collagen in the human body and plays a role in scar tissue formation. Collagen type 1 is also an important component of tendons and found in most connective tissues including cartilage. Collagen 1 is associated with several diseases including: Ehlers Danlos syndrome (arthrochalasia), Ehlers-Danlos syndrome (classical type), Osteogenesis imperfecta type 1-4 and osteoporosis.
References 1. Stuart J.M., et al. (2005) Arthritis Rheum. 26(7): 832-840.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273). Tissue: NIH/3T3. Species: Mouse. Primary Antibody: Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273). Tissue: NIH/3T3. Species: Mouse. Primary Antibody: Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273) at 1:100. The image on the right is treated with the synthesized peptide.

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  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

  ATTO 488 Fluorophore Excitation and Emission SpectraOptical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。