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当前位置： 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/Anti-Cdc7 Antibody (pThr376)/SPC-934D-A390/100-µl
商品详细StressMarq/Anti-Cdc7 Antibody (pThr376)/SPC-934D-A390/100-µl
StressMarq/Anti-Cdc7 Antibody (pThr376)/SPC-934D-A390/100-µl
StressMarq/Anti-Cdc7 Antibody (pThr376)/SPC-934D-A390/100-µl
商品编号： SPC-934D-A390
市场价： ¥6860.00
美元价： 4116.00
产地： 美国(厂家直采)
公司：
产品分类： 电泳缓冲液
公司分类： Electrophoretic_buffer
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name Cdc7 Antibody (pThr376)
Description

Rabbit Anti-Human Cdc7 (pThr376) Polyclonal

Species Reactivity Human
Applications WB, AM
Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen A phospho-specific peptide corresponding to residues surrounding Thr376 of human Cdc7 (AA373-379)
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Peptide Affinity Purified
Clonality Polyclonal
Specificity Detects 63.888 kDa.
Cite This Product StressMarq Biosciences Cat# SPC-934, RRID: AB_2707211
Certificate of Analysis A 1:250 dilution of SPC-934 was sufficient for detection of Cdc7 (pThr376) in 10 µg of human breast cancer cell (MCF7) lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names CDC7 cell division cycle 7 like 1 Antibody, Cell division cycle 7 kinase Antibody, HsCdc7 Antibody, Hsk 1 Antibody, HuCDC 7 Antibody
Research Areas Cancer, Cell Cycle, Cell Signaling, Epigenetics and Nuclear Signaling, Protein Phosphorylation
Cellular Localization Nucleus
Accession Number NP_003494
Gene ID 8317
Swiss Prot O00311
Scientific Background The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7/Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase a-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4. The kinase activity of the complex is regulated throughout the cell cycle, mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.

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  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol 

ATTO 390 Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission SpectraOptical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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