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StressMarq/StressXpress®过氧化氢酶活性试剂盒/SKT-215-192/2-x-96-well
StressMarq/StressXpress®过氧化氢酶活性试剂盒/SKT-215-192/2-x-96-well
商品编号：
SKT-215-192
市场价：
¥7700.00
美元价：
4620.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | CatalaseActivityKit |
| Description | Quantitativecolorimetricmeasurementofcatalaseactivity |
| SpeciesReactivity | SpeciesIndependent |
| Platform | Microplate |
| SampleTypes | Celllysates,EDTAPlasma,Erythrocytes,HeparinPlasma,Serum,Tissue |
| DetectionMethod | ColorimetricAssay |
| AssayType | DirectEnzymeActivityAssay |
| Utility | Colorimetricassayusedtoquantitativelymeasurecatalaseactivityinavarietyofsamples. |
| Sensitivity | 0.052U/ml |
| AssayRange | 0.156-5U/ml |
| Precision | IntraAssayPrecision:ThreehumanserumsamplesdilutedinAssayBufferwereruninreplicatesof20inanassay.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-1.71U/mL,3.5%CVSample2-0.84U/mL,4.0%CVSample3-0.48U/mL,4.8%CVInterAssayPrecision:ThreehumanserumsamplesdilutedinAssayBufferwereruninduplicatesintwenty-oneassaysrunovermultipledaysbythreeoperators.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-1.79U/mL,11.9%CVSample2-0.94U/mL,9.8%CVSample3-0.53U/mL,12.3%CV |
| IncubationTime | 45Minutes |
| NumberofSamples | 89samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | |||||||||||||||||||||
| ShippingTemperature | BlueIce | |||||||||||||||||||||
| ProductType | ActivityKits | |||||||||||||||||||||
| AssayOverview | TheCatalaseActivityKitisdesignedtoquantitativelymeasurecatalaseactivityinavarietyofsamples.Abovinecatalasestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffofthestandardcurve.SamplesaredilutedintheprovidedAssayBufferandaddedtothewellsofahalfareaclearplate.Hydrogenperoxideisaddedtoeachwellandtheplateincubatedatroomtemperaturefor30minutes.ThesuppliedColorimetricDetectionReagentisadded,followedbydilutedhorserADIshperoxidaseandincubatedatroomtemperaturefor15minutes.TheHRPreactswiththesubstrateinthepresenceofhydrogenperoxidetoconvertthecolorlesssubstrateintoapink-coloredproduct.Thecoloredproductisreadat560nm.IncreasinglevelsofcatalaseinthesamplescausesadecreaseinH2O2concentrationandareductioninpinkproduct.Theactivityofthecatalaseinthesampleiscalculatedaftermakingasuitablecorrectionforanydilution,usingsoftwareavailablewithmostplatereaders.TheresultsareexpressedintermsofunitsofcatalaseactivitypermL. | |||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | CatalaseActivityKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-215) |
BIOLOGicalDescription
| AlternativeNames | Cas1ActivityKit,CATActivityKit,Cs1ActivityKit,MGC138422ActivityKit,MGC138424ActivityKit |
| ResearchAreas | Cancer,Apoptosis,OxidativeStress |
| ScientificBackground | Hydrogenperoxide,H2O2isoneofthemostfrequentlyoccurringreactiveoxygenspecies.Itisformedeitherintheenvironmentorasaby-productofaerobicmetabolism,superoxideformationanddismutation,orasaproductofoxidaseactivity.Bothexcessivehydrogenperoxideanditsdecompositionproducthydroxylradical,formedinaFenton-typereaction,areharmfulformostcellcomponents.Itsrapidremovalisessentialforallaerobicallylivingprokaryoticandeukaryoticcells(1,2).Hydrogenperoxidehowevercanactasasecondmessengerinsignaltransductionpathways,inimmunecellactivation,inflammationprocesses,cellproliferation,andapoptosis(3-5).Oneofthemostefficientwaysofremovingperoxideisthroughtheenzymecatalase,whichisencodedbyasinglegene,andishighlyconservedamongspecies(6-8).Mammals,includinghumansandmice,expresscatalaseinalltissues,andahighconcentrationofcatalasecanbefoundintheliver,kidneysanderythrocytes(9,10).Theexpressionisregulatedattranscription,post-transcriptionandpost-translationlevels6,(11).Highcatalaseactivityisdetectedinperoxisomes(12).Morerecently,shortwavelengthUVradiationhasbeenshowntoproducereactiveoxygenspecies(ROS)throughtheactionofcatalase(13).ThisresponseisthoughttoactasamechanismtoprotectDNAbyconvertingdamagingUVradiationintoROSspeciesthatcanbemetabolizedanddetoxifiedbycellularantioxidantenzymes. |
| References | 1.CABIscolE,TamaritJ,RosJ.(2000)Int.Microbiol.3:3–8. 2.KirkmanHN,GaetaniGF.(2007)TrendsBiochemSci.32:44–50. 3.PeusD,etal.FreeRadic.(1999)Biol.Med.27:1197–1202. 4.RahmanI,andAdcockIM.(2006)Eur.Respir.J.28:219–242. 5.VealEA,DayAM,MorganBA.(2007)Mol.Cell.26:1–14. 6.Reimer,DL,Bailley,J,Singh,SM.(1994)Genomics.21:325–336. 7.Quan,.Korneluk,RGTropak,MB,Gravel,RA.(1986)NucleicAcidsRes.14:5321–5335. 8.Nakashima,H.,etal.(1989)Gene.79:279–288. 9.DeisSEROth,A,Dounce,AL.(1970)Physiol.Rev.50:319–375. 10.Schisler,NJ,Singh,SM.(1987)Genome.29:748–760. 11.Masters,C,Pegg,M,Crane,D.(1986)Mol.CellBiochem.70:113–120. 12.Chance,B,Sies,H,Boveris,A.(1979)Physiol.Rev.59:527–605. 13.Heck,DE,Vetrano,AM,Mariano,TM&Laskin,JD.(2003)J.Biol.Chem.278:22432–22436. |
ProductImages
TypicalStandardCurveforCatalaseActivityKit(EnzymeActivityAssay)StressXpress®–SKT-215.AssayType:DirectEnzyme.DetectionMethod:ColorimetricAssay.AssayRange:0.156–5U/ml.
Linearitywasdeterminedbytakingtwoserumsamples,onewithahighknowncatalaseactivityof1.163U/mLandtheotherwithalowercatalaseactivityof0.485U/mLandmixingthemingivenratios.Themeasuredactivitieswerecomparedtotheexpectedvaluesbasedontheratiosused.
CatalasecatalyzesthebreakdownofHydrogenPeroxide.
ProductCitations(1)
OtherCitations
MarkersofoxidativestressinumbilicalcordbloodfromG6PDdeficientAfricannewborns.
Stadem,P.S.etal.(2017)PLoSOne.12(2):e0172980.
PubMedID:28235023ReactivityHumanApplications:Umbilicalcordblood
品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
