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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-Caspase 3 Antibody (cleaved)/SPC-1319D-A390/100-µl
商品详细StressMarq/Anti-Caspase 3 Antibody (cleaved)/SPC-1319D-A390/100-µl
StressMarq/Anti-Caspase 3 Antibody (cleaved)/SPC-1319D-A390/100-µl
StressMarq/Anti-Caspase 3 Antibody (cleaved)/SPC-1319D-A390/100-µl
商品编号: SPC-1319D-A390
市场价: ¥6060.00
美元价: 3636.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameCaspase3Antibody(cleaved)
Description

RabbitAnti-HumanCaspase3(cleaved)Polyclonal

SpeciesReactivityHuman,Mouse,Rat
ApplicationsWB,IHC
AntibodyDilutionWB(1:2000),IHC(200);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenSynthesizedpeptidederivedfromhumanCaspase3.
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityPolyclonal
IsotypeIgG
SpecificityDetectsendogenouslevelsoffragmentofactivatedCaspase3resultingfromcleavageadjacenttoAsp175.
CiteThisProductStressMarqBiosciencesCat#SPC-1319,RRID:AB_2713529
CertificateofAnalysisA1:1000dilutionofSPC-1319wassufficientfordetectionofCaspase3in10µgofCOLOcelllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesApopainantibody,CASP-3antibody,CASP3antibody,CASP3_HUMANantibody,Caspase3antibody,caspase3,apoptosis-relatedcysteineproteaseantibody,Caspase-3subunitp12antibody,CPP32antibody,CPP-32antibody,CysteineproteaseCPP32antibody,PARPcleavageproteaseantibody,Procaspase3antibody,ProteinYamaantibody,SCA-1antibody,SCA1antibody,SREBPcleavageactivity1antibody,Yamaantibody,Yamaproteinantibody
CellularLocalizationCytoplasm
AccessionNumberNP_116786.1
GeneID836
SwissProtP42574
ScientificBackgroundCaspase-3isacaspaseproteinthatinteractswithcaspase-8andcaspase-9.ItisencodedbytheCASP3gene.CASP3orthologshavebeenidentifiedinnumerousmammalsforwhichcompletegenomedataareavailable.Uniqueorthologsarealsopresentinbirds,lizards,lissamphibians,andteleosts.Caspase-3isactivatedintheapoptoticcellbothbyextrinsic(deathligand)andintrinsic(mitochondrial)pathways.Thezymogenfeatureofcaspase-3isnecessarybecauseifunregulated,caspaseactivitywouldkillcellsindiscriminately.Caspase-3hasbeenfoundtobenecessaryfornormalbraindevelopmentaswellasitstypicalroleinapoptosis,whereitisresponsIBLeforchromatincondensationandDNAfragmentation.ElevatedlevelsofafragmentofCaspase-3,p17,inthebloodstreamisasignofarecentmyocardialinfarction.Itisnowbeingshownthatcaspase-3mayplayaroleinembryonicandhematopoieticstemcelldifferentiation.
References1.AlnemriES.etal.(1996)Cell87(2):171.
2.SalvesenGS.(2002)CellDeathDiffer.9(1):3–5.
3.GhavamiS(2009)J.Med.Genet.46(8):497–510.
4.PorterAGandJänickeRU(1999)CellDeathDiffer.6(2):99–104.
5.AgostoM(2011)J.Am.Coll.Cardiol.57(2):220–1.
6.ABDul-GhaniM.(2008)CellStemCell2(6):515–6.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-Caspase 3 Polyclonal Antibody (SPC-1319). Tissue: Ehrlich Ascites. Species: Mouse. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Caspase 3 Polyclonal Antibody (SPC-1319) at 1:100.</p>

ImmunohistochemistryanalysisusingRabbitAnti-Caspase3PolyclonalAntibody(SPC-1319).Tissue:EhrlichAscites.Species:Mouse.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-Caspase3PolyclonalAntibody(SPC-1319)at1:100.

<p>Western blot analysis of Human COLO cell lysates showing detection of ~17kDa Caspase 3 protein using Rabbit Anti-Caspase 3 Polyclonal Antibody (SPC-1319). Lane 1: Human COLO cell lysate treated with the immunizing peptide. Lane 2: Human COLO cell lysate. Primary Antibody: Rabbit Anti-Caspase 3 Polyclonal Antibody (SPC-1319) at 1:1000. Predicted/Observed Size: ~17kDa.</p>

WesternblotanalysisofHumanCOLOcelllysatesshowingdetectionof~17kDaCaspase3proteinusingRabbitAnti-Caspase3PolyclonalAntibody(SPC-1319).Lane1:HumanCOLOcelllysatetreatedwiththeimmunizingpeptide.Lane2:HumanCOLOcelllysate.PrimaryAntibody:RabbitAnti-Caspase3PolyclonalAntibody(SPC-1319)at1:1000.Predicted/ObservedSize:~17kDa.

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 ATTO390
Overview:
  • Highfluorescenceyield
  • LargeStokes-shift(89nm)
  • GoodphotostABIlity
  • Moderatelyhydrophilic
  • Goodsolubilityinpolarsolvents
  • Coumarinderivate,uncharged
  • Lowmolarmass:343.42g/mol 

ATTO390Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission SpectraOpticalProperties:

λex =390nm

λem=479nm

εmax=2.4×104

Φf= 0.90

τfl =5.0ns

Brightness= 21.6

Laser =365or405nm

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。