StressMarq/Anti-Calnexin-CT Antibody/SPC-108A/50-µl-免疫在线蚂蚁淘旗下平台

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商品详细StressMarq/Anti-Calnexin-CT Antibody/SPC-108A/50-µl

StressMarq/Anti-Calnexin-CT Antibody/SPC-108A/50-µl

商品编号： SPC-108A

品牌： StressMarq Biosciences

市场价： ¥2960.00

美元价： 1776.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName Calnexin-CTAntibody

Description

RabbitAnti-DogCalnexin-CTPolyclonal

SpeciesReactivity Dog,Human,Monkey,Mouse,Rat,Africanclawedfrog(Xenopuslaevis),Avian,Bovine,Chicken,FruitFly(Drosophilamelanogaster),GuineaPig(Caviaporcellus),Hamster,Pig,Quail,Rabbit,Sheep

Applications WB,ICC/IF,IHC

AntibodyDilution WB(1:1000),IHC(1:100),ICC/IF(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Rabbit

ImmunogenSpecies Dog

Immunogen DogcalnexinC-terminalsyntheticpeptideconjugatedtoKLH.Identicaltohuman,mouseandratcalnexinsequencesovertheseresidues.

Concentration 1mg/ml

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-ProteinComplex
Smallphycobiliprotein
Isolatedfromredalgae
Largestokesshift(195nm)
MolecularWeight:35kDa

PerCPDatasheet

PerCP Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =482nm

λ_em=677nm

ε_max=1.96x10⁶

Laser =488nm

PE/ATTO594

PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.

Overview:

Highfluorescenceyield
HighphotostABIlity
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation

PE/ATTO594Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

OpticalProperties:

λ_ex =535nm

λ_em =627 nm

Laser = 488to561nm

FITC(Fluorescein)

Overview:

Excellentfluorescencequantumyield
Highrateofphotobleaching
Goodsolubilityinwater
Broademissionspectrum
pHdependentspectra
Molecularformula:C₂₀H₁₂O₅
Molarmass:332.3g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =494 nm

λ_em=520 nm

ε_max=7.3×10⁴

Φ_f= 0.92

τ_fl =5.0ns

Brightness= 67.2

Laser = 488 nm

Filterset = FITC

ATTO700

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:575g/mol

ATTO700Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =700 nm

λ_em=719nm

ε_max=1.25×10⁵

Φ_f= 0.25

τ_fl =1.6ns

Brightness= 31.3

Laser =676nm

Filterset =Cy®5.5

ATTO680

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:631g/mol

ATTO680Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =680nm

λ_em=700 nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.7ns

Brightness=37.5

Laser =633 –676nm

Filterset =Cy®5.5

ATTO655

Overview:

Highfluorescenceyield
Highthermalandphotostability
Excellentozoneresistance
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:634g/mol

ATTO655Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =663nm

λ_em=684nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.8ns

Brightness= 37.5

Laser =633 –647nm

Filterset =Cy®5

ATTO633

Overview:

Highfluorescenceyield
Highthermalandphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
StableatpH4–11
Cationicdye,perchloratesalt
MolarMass:652.2g/mol

ATTO633Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =629nm

λ_em=657nm

ε_max=1.3×10⁵

Φ_f= 0.64

τ_fl =3.2ns

Brightness=83.2

Laser =633 nm

Filterset =Cy®5

ATTO594

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:1137g/mol

ATTO594Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

OpticalProperties:

λ_ex =601 nm

λ_em=627nm

ε_max=1.2×10⁵

Φ_f= 0.85

τ_fl =3.5ns

Brightness=102

Laser =594nm

Filterset =TexasRed®

ATTO565

Overview:

Highfluorescenceyield
Highthermalandphotostability
Goodsolubilityinpolarsolvents
Excellentsolubilityinwater
Verylittleaggregation
Rhodaminedyederivative
MolarMass:611g/mol

ATTO565Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =563nm

λ_em=592nm

ε_max=1.2×10⁵

Φ_f= 0.9

τ_fl =3.4n

Brightness=10

Laser =532nm

Filterset = TRITC

ATTO488

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:804g/mol

ATTO488Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =501nm

λ_em=523nm

ε_max=9.0×10⁴

Φ_f= 0.80

τ_fl =4.1ns

Brightness= 72

Laser = 488 nm

Filterset = FITC

ATTO390

Overview:

Highfluorescenceyield
LargeStokes-shift(89nm)
Goodphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
Coumarinderivate,uncharged
Lowmolarmass:343.42g/mol

ATTO390Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

OpticalProperties:

λ_ex =390nm

λ_em=479nm

ε_max=2.4×10⁴

Φ_f= 0.90

τ_fl =5.0ns

Brightness= 21.6

Laser =365or405nm

APC(Allophycocyanin)

Overview:

Highquantumyield
Largephycobiliprotein
6chromophorespermolecule
Isolatedfromredalgae
MolecularWeight:105kDa

APCDatasheet

APC Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =650nm

λ_em=660nm

ε_max=7.0×10⁵

Φ_f= 0.68

Brightness= 476

Laser =594or633 nm

Filterset =Cy®5

Streptavidin

Properties:

Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinity
Molecularweight:53kDa
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

StreptavidinDatasheet

Biotin

Properties:

BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity
Molarmass:244.31g/mol
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

BiotinDatasheet

HRP(HorserADIshperoxidase)

Properties:

Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
Readilycombineswithhydrogenperoxide(H₂O₂)toformHRP-H₂O₂complexwhichcanoxidizevarioushydrogendonors
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
Highturnoverrateenablesrapidgenerationofastrongsignal
44kDaglycoprotein
Extinctioncoefficient:100(403nm)
Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

AP(AlkalinePhosphatase)

Properties:

Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols
Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts
- Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts
Molecularweight:140kDa
Applications:Westernblot,immunohistochemistry,andELISA

APDatasheet

R-PE(R-Phycoerythrin)

Overview:

Broadexcitationspectrum
Highquantumyield
Photostable
Memberofthephycobiliproteinfamily
Isolatedfromredalgae
Excellentsolubilityinwater
MolecularWeight:250kDa

R-PEDatasheet

R-PE Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =565nm

λ_em=575nm

ε_max=2.0×10⁶

Φ_f= 0.84

Brightness=1.68x10³

Laser = 488to561nm

Filterset = TRITC

Properties

StorageBuffer	PBSpH7.4,50%glycerol,0.09%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	PeptideAffinityPurified
Clonality	Polyclonal
Specificity	DetectstheC-terminaldomainofCalnexin~90kDa.WeakdetectioninChicken,Drosophila,andXenopustissues
CiteThisProduct	StressMarqBiosciencesCat#SPC-108,RRID:AB_2069000
CertificateofAnalysis	A1:1000dilutionofSPC-108wassufficientfordetectionofCalnexinin10µgofHeLacelllysatebyECLimmunoblotanalysis.

BIOLOGicalDescription

AlternativeNames	Calnexinantibody,CALX_HUMANantibody,CANXantibody,CNXantibody,FLJ26570antibody,HistocompatibilitycomplexclassIantigenbindingproteinp88antibody,IP90antibody,MajorhistocompatibilitycomplexclassIantigen-bindingproteinp88antibody,P90antibody
ResearchAreas	CellSignaling,OrganelleMarkers,TagsandCellMarkers
CellularLocalization	Cytoplasm,EndoplasmicReticulum,Endoplasmicreticulumlumen,Melanosome
AccessionNumber	NP_001003232.1
GeneID	403908
SwissProt	P24643
ScientificBackground	Calnexin,anabundant~90kDaintegralproteinoftheendoplasmicreticulum,isalsoreferredtoasIP90,p88andp90(1).Itconsistsofalarge50kDaN-terminalcalcium-bindingluminaldomain,asingletransmembranehelixandashortacidiccytoplasmictail(2,3).UnlikeitsERcounterpartswhichhaveaKDELsequenceontheirC-terminustoensureERretention(4),calnexinhaspositivelychargedcytosolicresiduesthatdothesamething(3).MostERproteinsactasmolecularchaperonesandparticipateintheproperfoldingofpolypeptidesandtheirassemblyintomulti-subunitproteins.Calnexintogetherwithcalreticulin,playsakeyroleinglycoproteinfoldinganditscontrolwithintheER,byinteractingwithfoldingintermediatesviatheirmono-glycosylatedglycans(5,6).CalnexinhasalsobeenshowntoassociatewiththemajorhistocompatibilitycomplexclassIheavychains,partialcomplexesoftheTcellreceptorandBcellmembraneimmunoglobulin(7).
References	1.RajagopalanS.,XuY.,andBrennerM.B.(1994)Science263(5145):387-90. 2.TjoelkerL.W.,etal.(1994)Biochemistry33:3229. 3.SchragJ.etal.(2001)MolecularCell8(3):633-644. 4.JaniszewskiM.(2005)J.BiolChem.280(49):40813-40819. 5.ElagozA.,CallejoM.,ArmstrongJ.,andRokeachL.A.(1999)J.CellSci.112:4449-4460. 6.OttekenA.andMossB.(1996)JBioChem.271(1):97-103. 7.GalvinK.etal.(1992)ProcNatlAcadSciUSA.89(18):8452-6.

ProductImages

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108). Tissue: HeLa cells. Species: Human. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-CalnexinPolyclonalAntibody(SPC-108).Tissue:HeLacells.Species:Human.PrimaryAntibody:RabbitAnti-CalnexinPolyclonalAntibody(SPC-108)at1:100.SecondaryAntibody:FITCGoatAnti-Rabbit(green).

<p>Western blot analysis of Human HeLa cell lysates showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108). Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108) at 1:1000.</p>

WesternblotanalysisofHumanHeLacelllysatesshowingdetectionofCalnexinproteinusingRabbitAnti-CalnexinPolyclonalAntibody(SPC-108).PrimaryAntibody:RabbitAnti-CalnexinPolyclonalAntibody(SPC-108)at1:1000.

<p>Immunohistochemistry analysis using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT.</p>

ImmunohistochemistryanalysisusingRabbitAnti-CalnexinPolyclonalAntibody(SPC-108).Tissue:backskin.Species:Mouse.Fixation:Bouin’sFixativeSolution.PrimaryAntibody:RabbitAnti-CalnexinPolyclonalAntibody(SPC-108)at1:100for1houratRT.SecondaryAntibody:FITCGoatAnti-Rabbit(green)at1:50for1houratRT.

<p>Western blot analysis of Rat Tissue lysates showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108). Load: 15 µg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.</p>

WesternblotanalysisofRatTissuelysatesshowingdetectionofCalnexinproteinusingRabbitAnti-CalnexinPolyclonalAntibody(SPC-108).Load:15µgprotein.Block:1.5%BSA.PrimaryAntibody:RabbitAnti-CalnexinPolyclonalAntibody(SPC-108)at1:1000for2hoursatRT.SecondaryAntibody:DonkeyAnti-RabbitIgG:HRPfor1houratRT.

ProductCitations(9)

WesternBlot

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PubMedID:27635674ReactivityHumanApplications:WesternBlot

Immunohistochemistry

Nucleobindin1(NUCB1)isaGolgi-residentmarkerofneurons.

Tulke,S.etal.(2015)Neuroscience.314:179-88.

PubMedID:26666627ReactivityRatApplications:Immunohistochemistry

Immunocytochemistry/Immunofluorescence

ArecombinantDNAvaccineprotectsmicedeficientinthealpha/betainterferonreceptoragainstlethalchallengewithUsutuvirus.

Martín-Acebe,sM.A.etal.(2016)Vaccine.34(18):2066-73.

PubMedID:26993334ReactivityMouseApplications:Immunocytochemistry/Immunofluorescence

FunctionalRescueofTrafficking-ImpairedABCB4MutantsbyChemicalChaperones.

Gordo-Gilart,R.,Andueza,S.,Hierro,L.,Jara,P.andAlvarez,L.(2016)PLoSOne.11(2):e0150098.

PubMedID:26900700ReactivityDogApplications:Immunocytochemistry/Immunofluorescence

CCDC115DeficiencyCausesaDisorderofGolgiHomeostasiswithAbnormalProteinGlycosylation

Jansen,J.C.etal.(2016)AmJHumGenet.98(2):310-21.

PubMedID:26833332ReactivityHumanApplications:Immunocytochemistry/Immunofluorescence

HeterozygousABCB4mutationsinchildrenwithcholestaticliverdisease.

Gordo-Gilart,R.etal.(2015)LiverInt.36(2):258-67.

PubMedID:26153658ReactivityHumanApplications:Immunocytochemistry/Immunofluorescence

FunctionalanalysisofABCB4mutationsrelatesclinicaloutcomesofprogressivefamilialintrahepaticcholestasistype3tothedegreeofMDR3floppaseactivity.

Gordo-Gilart,R.etal.(2014)Gut.64(1):147-55.

PubMedID:24594635ReactivityHumanApplications:Immunocytochemistry/Immunofluorescence

Expressionofnucleobindin1(NUCB1)inpancreaticisletsandotherendocrinetissues.

Williams,P.,Tulke,S.,Ilegems,E.,Berggren,P.O.,Broberger,C.(2014)CellTissueRes.358(2):331-42.

PubMedID:25038744ReactivityMouseApplications:Immunocytochemistry/Immunofluorescence

TheCompositionofWestNilevirusLipidEnvelopeUnveilsaRoleofSphingolipidMetabolismonFlavivirusBiogenesis.

Martín-Acebes,M.A.etal.(2014)JVirol.88(20):12041-54.

PubMedID:25122799ReactivityHumanApplications:Immunocytochemistry/Immunofluorescence

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