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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-BNIP3 Antibody/SPC-685D-ALP/100-µg
商品详细StressMarq/Anti-BNIP3 Antibody/SPC-685D-ALP/100-µg
StressMarq/Anti-BNIP3 Antibody/SPC-685D-ALP/100-µg
StressMarq/Anti-BNIP3 Antibody/SPC-685D-ALP/100-µg
商品编号: SPC-685D-ALP
市场价: ¥6720.00
美元价: 4032.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name BNIP3 Antibody
Description

Rabbit Anti-Human BNIP3 Polyclonal

Species Reactivity Human, Mouse
Applications WB, ICC/IF
Antibody Dilution WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthetic peptide from the C-terminal of human BNIP3
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Peptide Affinity Purified
Clonality Polyclonal
Specificity Predicted molecular weight at ~21.5kDa. Observed bands at ~20-30kDa and 50-60kDa due to the homodimer complex of BNIP3.
Cite This Product StressMarq Biosciences Cat# SPC-685, RRID: AB_2705699
Certificate of Analysis A 1:1000 dilution of SPC-685 was sufficient for detection of BNIP3 on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names NIP3 Antibody, BCL2 Adenovirus E1B 19kDa Interacting Protein 3 Antibody, BNIP3_Human Antibody
Research Areas Cancer, Apoptosis, Cardiovascular System, Cell Signaling, Heart, Hypoxia, Metabolism, Metabolism processes, Mitochondrial Markers, Mitochondrial Metabolism
Cellular Localization Mitochondrion, Mitochondrion outer membrane
Accession Number NP_004043.3
Gene ID 664
Swiss Prot Q12983
Scientific Background BNIP3 is an apoptosis-inducing protein which can overcome BCL2 suppression. It may play a role in repartioning calcium between the two major intracellular calcium stores in association with BCL2. It has been shown to interact with CD47, BCL2-like, and BCL-2 (1-3).
References 1. Boyd J.M., et al. (1994) Cell. 79(2): 341-351.
2. Lamy L., et al. (2003) J Biol Chem. 278(26): 23915-23921.
3. Ray R., et al. (2000) J Biol Chem. 275(2): 1439-1448.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Tissue: Fibroblast cell line (NIH 3T3). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BNIP3 Antibody (D) Composite.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Tissue: Fibroblast cell line (NIH 3T3). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BNIP3 Antibody (D) Composite.

<p>Western blot analysis of Human HeLa and 293T cell lysates showing detection of ~30kDa  BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa .</p>

Western blot analysis of Human HeLa and 293T cell lysates showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa .

<p>Western blot analysis of Mouse Kidney showing detection of ~30kDa  BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa . Other Band(s): ~60kDa (BNIP3 complex).</p>

Western blot analysis of Mouse Kidney showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa . Other Band(s): ~60kDa (BNIP3 complex).

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AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。