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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-BIM/BOD (IN2) Antibody/SPC-164C/25-µg
商品详细StressMarq/Anti-BIM/BOD (IN2) Antibody/SPC-164C/25-µg
StressMarq/Anti-BIM/BOD (IN2) Antibody/SPC-164C/25-µg
StressMarq/Anti-BIM/BOD (IN2) Antibody/SPC-164C/25-µg
商品编号: SPC-164C
市场价: ¥2780.00
美元价: 1668.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameBIM/BOD(IN2)Antibody
Description

RabbitAnti-HumanBIM/BOD(IN2)Polyclonal

SpeciesReactivityHuman,Mouse,Rat
ApplicationsWB,ICC/IF
AntibodyDilutionWB(1:400),ICC/IF(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenInternalcentralaminoacidsofhumanBim
Concentration1mg/ml
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBS,50%glycerol,0.09%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationProteinApurified
ClonalityPolyclonal
SpecificityDetects~23kDa.
CiteThisProductStressMarqBiosciencesCat#SPC-164,RRID:AB_2290429
CertificateofAnalysis2.5µg/mlofSPC-164wassufficientfordetectionofBimin20µgofK562celllysatebycolorimetricimmunoblotanalysisusingGoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesBCL2likeprotein11Antibody,Bcl2interactingmediatorofcelldeathAntibody,Bcl2interactingproteinBimAntibody,BCL2like11Antibody,BAMAntibody,BimElAntibody,BimLAntibody,BODAntibody,BIMbeta6Antibody,BIMBeta7Antibody,BIMalpha3Antibody,BIMalpha4Antibody,BIMalpha5Antibody,BIMalpha6Antibody
ResearchAreasCancer,Apoptosis
CellularLocalizationEndomembranesystem,Mitochondrion
AccessionNumberNP_619527.1
GeneID10018
SwissProtO43521
ScientificBackgroundMembersintheBcl-2familyarecriticalregulatorsofapoptosisbyeitherinhibitingorpromotingcelldeath.Bim/BODisagroupofthreesplicevariants,BimEL,BimLandBimS,withapparentmolecularmassesof~23,16,and13kDa,respectively.Bcl-2homology3(BH3)domainisapotentdeathdomain.BH3domaincontainingpro-apoptoticproteins,includingBad,Bax,Bid,Bik,andHrk,formagrowingsubclassoftheBcl-2family.AnovelBH3domaincontainingproteinwasrecentlyidentifiedanddesignatedBimorBODinhuman,mouseandrat(1,2).Bim/BODinteractswithdiversemembersinthepro-survivalBcl-2sub-familyincludingBcl-2,Bcl-xLandBcl-w.Bim/BODinducesapoptosis.ThemessengerRNAofBimisubiquitouslyexpressedinmultipletissuesandcelllines(1,2).
References1.O"ConnorL.,StrasserA.,O"ReillyL.A.,etal.(1998)EMBOJ.17:384-395.
2.HsuS.Y.,LinP.,andHsuehA.J.(1998)Mol.Endocrinol.12:1432-40.

ProductImages

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BIM Polyclonal Antibody (SPC-164). Tissue: K562 cells. Species: Human. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-164) at 1:100.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-BIMPolyclonalAntibody(SPC-164).Tissue:K562cells.Species:Human.PrimaryAntibody:RabbitAnti-BIMPolyclonalAntibody(SPC-164)at1:100.

<p>Western blot analysis of Human A549 cell lysates showing detection of BIM protein using Rabbit Anti-BIM Polyclonal Antibody (SPC-164). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-164) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.</p>

WesternblotanalysisofHumanA549celllysatesshowingdetectionofBIMproteinusingRabbitAnti-BIMPolyclonalAntibody(SPC-164).Load:15µgprotein.Block:1.5%BSAfor30minutesatRT.PrimaryAntibody:RabbitAnti-BIMPolyclonalAntibody(SPC-164)at1:1000for2hoursatRT.SecondaryAntibody:DonkeyAnti-RabbitIgG:HRPfor1houratRT.

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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。