
Overview:
Product Name | Amyloid Oligomers (A11) Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Rabbit Anti-Human Amyloid Oligomers (A11) Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse, Rat, Eukaryote | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, IHC, ICC/IF, IP, ELISA | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000), IP (1:1000); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Synthetic molecular mimic of soluble oligomers | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein A purified |
Clonality | Polyclonal |
Specificity | Recognizes all types of amyloid oligomers. Appears to recognize a peptide backbone epitope that is common to amyloid oligomers, but is not found in native proteins, amyloidogenic monomer or mature a |
Cite This Product | StressMarq Biosciences Cat# SPC-506, RRID: AB_10962958 |
Certificate of Analysis | A 1:1000 dilution of SPC-506 was sufficient for detection of amyloid oligomers in 10 µg of mouse brain lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | Amyloid Oligomer alpha beta Antibody, A11 Antibody, Amyloid Oligomer AlphaBeta Antibody, APP Antibody |
Research Areas | Blood, Cardiovascular System, Cell Signaling, Neuroscience |
Cellular Localization | Membrane |
Accession Number | NM_000484.2 |
Gene ID | 9606 |
Swiss Prot | P05067 |
Scientific Background | Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres (1, 2). These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer’s symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson’s disease, and accumulation of polyglutamine-containing aggregates in Huntington’s disease (2, 3). |
References |
1. Glabe C.G. (2004) Trends Biochem Sci. 29(10): 542-547. 2. Kayed R., et al. (2004) J Bio. Chem. 279: 46363-46366. 3. Kayed R., et al. (2003) Science. 300(5618): 486-489. |
Product Images

Immunohistochemistry analysis using Rabbit Anti-Amyloid Oligomers (A11) Polyclonal Antibody (SPC-506). Tissue: Alzheimer’s Disease brain. Species: Human. Fixation: Formalin fixed. Primary Antibody: Rabbit Anti-Amyloid Oligomers (A11) Polyclonal Antibody (SPC-506) at 1:1000. Secondary Antibody: Goat Anti-Rabbit ATTO 594 (red). Localization: Plaque. (A) Amyloid Fibril (OC) Antibody (SPC-507). (B) Amyloid Oligomer (A11) Antibody (SPC-506). (C) Composite. Courtesy of: Dr. Elizabeth Head, University of California, Irvine.

Western blot analysis of Human Abeta42 fibrils and prefibrillar oligomers showing detection of Amyloid Oligomers (A11) protein using Rabbit Anti-Amyloid Oligomers (A11) Polyclonal Antibody (SPC-506). Primary Antibody: Rabbit Anti-Amyloid Oligomers (A11) Polyclonal Antibody (SPC-506) at 1:1000. Courtesy of: Kayed, R., Head, E., Thompson, J. L., McIntire, T. M., Milton, S. C., Cotman, C. W., et al. (2003). Common structure of soluble amyloid oligomers implies common mechanism of pathogenesis. Science 300, 486489. doi: 10.1126/science.1079469.

Dot blot analysis using Rabbit Anti-Amyloid Oligomers (A11) Polyclonal Antibody (SPC-506). Tissue: Abeta42 fibrils and prefibrillar oligomers. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Oligomers (A11) Polyclonal Antibody (SPC-506) at 1:1000. Courtesy of: Kayed, R., Head, E., Thompson, J. L., McIntire, T. M., Milton, S. C., Cotman, C. W., et al. (2003). Common structure of soluble amyloid oligomers implies common mechanism of pathogenesis. Science 300, 486489. doi: 10.1126/science.1079469.
Product Citations (1)
Immunohistochemistry
Acute amnestic encephalopathy in amyloid-ß oligomers injected mice is due to their widespread diffusion in vivo.
Epelbaum, S. et al. (2015) Neurobiol Aging. 36(6):2043-52.
PubMed ID: 25862419 Reactivity Human Applications: Immunohistochemistry
ATTO 390 | ||
Overview:
ATTO 390 Datasheet | ![]() | Optical Properties: λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |