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当前位置: 首页 > 产品中心 > hydroxybenzene > StressMarq/Alpha Synuclein Protein/SPR-316C/200-µg
商品详细StressMarq/Alpha Synuclein Protein/SPR-316C/200-µg
StressMarq/Alpha Synuclein Protein/SPR-316C/200-µg
StressMarq/Alpha Synuclein Protein/SPR-316C/200-µg
商品编号: SPR-316C
市场价: ¥10880.00
美元价: 6528.00
产地: 美国(厂家直采)
公司:
产品分类:
公司分类: hydroxybenzene
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameAlphaSynucleinProtein
Description

HumanRecombinantAlphaSynucleinProteinMonomer(Control)

ApplicationsWB,SDS-PAGE,Invivoassay,Invitroassay
ConcentrationLot/batchspecific.Seeincludeddatasheet.
ConjugatesNotag

Properties

StorageBuffer0.2µmfilteredsolutionin20mMtris,150mMNaClpH7.5
StorageTemperature-80ºC
ShippingTemperatureBlueIceor4ºC
PurificationIon-exchangePurified
Specificity~14.46kDa
CiteThisProductHumanRecombinantAlphaSynucleinProtein(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SPR-316)
CertificateofAnalysisCertified92%pureusingSDS-PAGEanalysis.

BIOLOGicalDescription

AlternativeNamesAlphasynucleinmonomer,Alpha-synucleinmonomer,Alphasynucleinproteinmonomer,Alphasynucleinmonomer,Alpha-synucleinprotein,Non-AbetacomponentofADamyloidprotein,Non-A4componentofamyloidprecursorprotein,NACPprotein,SNCAprotein,NACPprotein,PARK1protein,Alphasynucleinmonomers,SYNprotein,Parkisondiseasefamilial1Protein
ResearchAreasAlzheimer"sDisease,Neurodegeneration,Neuroscience,Parkinson"sDisease
CellularLocalizationCytoplasm,Membrane,Nucleus
AccessionNumberNP_000336.1
GeneID6622
SwissProtP37840
ScientificBackgroundAlpha-Synuclein(SNCA)isexpressedpredominantlyinthebrain,whereitisconcentratedinpresynapticnerveterminals(1).Alpha-synucleinishighlyexpressedinthemitochondriaoftheolfactorybulb,hippocampus,striatumandthalamus(2).Functionally,ithasbeenshowntosignificantlyinteractwithtubulin(3),andmayserveasapotentialmicrotubule-associatedprotein.Ithasalsobeenfoundtobeessentialfornormaldevelopmentofthecognitivefunctions;inactivationmayleadtoimpairedspatiallearningandworkingmemory(4).SNCAfibrillaraggregatesrepresentthemajornonA-betacomponentofAlzheimersdiseaseamyloidplaque,andamajorcomponentofLewybodyinclusions,andParkinson’sdisease.Parkinson"sdisease(PD)isacommonneurodegenerativedisordercharacterizedbytheprogressiveaccumulationinselectedneuronsofproteininclusionscontainingalpha-synucleinandubiquitin(5,6).
References1.“GeneticsHomeReference:SNCA”.USNationalLibraryofMedicine.(2013).
2.ZhangL.,etal.(2008)BrainRes.1244:40-52.
3.AlimM.A.,etal.(2002)JBiolChem.277(3):2112-2117.
4.KokhanV.S.,AfanasyevaM.A.,Van’kinG.(2012)Behav.Brain.Res.231(1):226-230.
5.SpillantiniM.G.,etal.(1997)Nature.388(6645):839-840.
6.MezeyE.,etal.(1998)NatMed.4(7):755-757.

ProductImages

<p>SDS-PAGE of ~14 kDa Human Recombinant Alpha Synuclein Protein Monomer (Control) (SPR-316). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (2.5 µg). Lane 3: BSA (5 µg). Lane 4: Alpha Synuclein Protein Monomer (2.5 µg) (SPR-316). Lane 5: Alpha Synuclein Protein Monomer (5 µg) (SPR-316).</p>

SDS-PAGEof~14kDaHumanRecombinantAlphaSynucleinProteinMonomer(Control)(SPR-316).Lane1:MolecularWeightLadder(MW).Lane2:BSA(2.5µg).Lane3:BSA(5µg).Lane4:AlphaSynucleinProteinMonomer(2.5µg)(SPR-316).Lane5:AlphaSynucleinProteinMonomer(5µg)(SPR-316).

<p>Active alpha synuclein aggregate (SPR-322) seeds the formation of new alpha Synuclein aggregates from the pool of active alpha Synuclein monomers (SPR-321). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (SPR-322) is combined with 100 µM of active alpha Synuclein monomer (SPR-321), as compared to when 100 µM of control alpha Synuclein monomer (SPR-316) is combined with 10 nM of active alpha Synuclein aggregate (SPR-322). Thioflavin T ex = 450 nm, em = 485 nm.</p>

Activealphasynucleinaggregate(SPR-322)seedstheformationofnewalphaSynucleinaggregatesfromthepoolofactivealphaSynucleinmonomers(SPR-321).ThioflavinTisafluorescentdyethatbindstobetasheet-richstructures,suchasthoseinalphaSynucleinaggregates.Uponbinding,theemissionspectrumofthedyeexperiencesared-shift,andincreasedfluorescenceintensity.ThioflavinTemissioncurvesshowincreasedfluorescence(correlatedtoalphaSynucleinproteinaggregation)overtimewhen10nMofactivealphaSynucleinaggregate(SPR-322)iscombinedwith100µMofactivealphaSynucleinmonomer(SPR-321),ascomparedtowhen100µMofcontrolalphaSynucleinmonomer(SPR-316)iscombinedwith10nMofactivealphaSynucleinaggregate(SPR-322).ThioflavinTex=450nm,em=485nm.

<p>Active alpha synuclein aggregate (SPR-322) seeds the formation of new alpha Synuclein aggregates from the pool of active alpha Synuclein monomers (SPR-321). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (SPR-322) is combined with 100 µM of active alpha Synuclein monomer (SPR-321), as compared to active alpha Synuclein aggregate (SPR-322) and active alpha Synuclein monomer (SPR-321) alone. Thioflavin T ex = 450 nm, em = 485 nm.</p>

Activealphasynucleinaggregate(SPR-322)seedstheformationofnewalphaSynucleinaggregatesfromthepoolofactivealphaSynucleinmonomers(SPR-321).ThioflavinTisafluorescentdyethatbindstobetasheet-richstructures,suchasthoseinalphaSynucleinaggregates.Uponbinding,theemissionspectrumofthedyeexperiencesared-shift,andincreasedfluorescenceintensity.ThioflavinTemissioncurvesshowincreasedfluorescence(correlatedtoalphaSynucleinproteinaggregation)overtimewhen10nMofactivealphaSynucleinaggregate(SPR-322)iscombinedwith100µMofactivealphaSynucleinmonomer(SPR-321),ascomparedtoactivealphaSynucleinaggregate(SPR-322)andactivealphaSynucleinmonomer(SPR-321)alone.ThioflavinTex=450nm,em=485nm.

<p>Active alpha synuclein aggregate (SPR-322) seeds the formation of new alpha Synuclein aggregates from the pool of active alpha Synuclein monomers (SPR-321). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (SPR-322) is combined with 100 µM of active alpha Synuclein monomer (SPR-321), as compared to when 100 µM of active alpha Synuclein monomer (SPR-321) is combined with 10 nM of control alpha Synuclein aggregate (SPR-317), or 100 µM of control alpha Synuclein monomer (SPR-316) is combined with 10 nM of control alpha Synuclein aggregate (SPR-317). Thioflavin T ex = 450 nm, em = 485 nm.</p>

Activealphasynucleinaggregate(SPR-322)seedstheformationofnewalphaSynucleinaggregatesfromthepoolofactivealphaSynucleinmonomers(SPR-321).ThioflavinTisafluorescentdyethatbindstobetasheet-richstructures,suchasthoseinalphaSynucleinaggregates.Uponbinding,theemissionspectrumofthedyeexperiencesared-shift,andincreasedfluorescenceintensity.ThioflavinTemissioncurvesshowincreasedfluorescence(correlatedtoalphaSynucleinproteinaggregation)overtimewhen10nMofactivealphaSynucleinaggregate(SPR-322)iscombinedwith100µMofactivealphaSynucleinmonomer(SPR-321),ascomparedtowhen100µMofactivealphaSynucleinmonomer(SPR-321)iscombinedwith10nMofcontrolalphaSynucleinaggregate(SPR-317),or100µMofcontrolalphaSynucleinmonomer(SPR-316)iscombinedwith10nMofcontrolalphaSynucleinaggregate(SPR-317).ThioflavinTex=450nm,em=485nm.

<p>Active alpha synuclein aggregate (SPR-322) seeds the formation of new alpha Synuclein aggregates from the pool of active alpha Synuclein monomers (SPR-321). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (SPR-322) is combined with 100 µM of active alpha Synuclein monomer (SPR-321), as compared to when 100 µM of control alpha Synuclein monomer (SPR-316) is combined with 10 nM of active alpha Synuclein aggregate (SPR-322), or active alpha Synuclein aggregate (SPR-322) and active alpha Synuclein monomer (SPR-321) alone. Thioflavin T ex = 450 nm, em = 485 nm.</p>

Activealphasynucleinaggregate(SPR-322)seedstheformationofnewalphaSynucleinaggregatesfromthepoolofactivealphaSynucleinmonomers(SPR-321).ThioflavinTisafluorescentdyethatbindstobetasheet-richstructures,suchasthoseinalphaSynucleinaggregates.Uponbinding,theemissionspectrumofthedyeexperiencesared-shift,andincreasedfluorescenceintensity.ThioflavinTemissioncurvesshowincreasedfluorescence(correlatedtoalphaSynucleinproteinaggregation)overtimewhen10nMofactivealphaSynucleinaggregate(SPR-322)iscombinedwith100µMofactivealphaSynucleinmonomer(SPR-321),ascomparedtowhen100µMofcontrolalphaSynucleinmonomer(SPR-316)iscombinedwith10nMofactivealphaSynucleinaggregate(SPR-322),oractivealphaSynucleinaggregate(SPR-322)andactivealphaSynucleinmonomer(SPR-321)alone.ThioflavinTex=450nm,em=485nm.

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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。