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当前位置: 首页 > 产品中心 > hydroxybenzene > StressMarq/Alpha Synuclein Protein/SPR-317E/500-µg
商品详细StressMarq/Alpha Synuclein Protein/SPR-317E/500-µg
StressMarq/Alpha Synuclein Protein/SPR-317E/500-µg
StressMarq/Alpha Synuclein Protein/SPR-317E/500-µg
商品编号: SPR-317E
市场价: ¥22100.00
美元价: 13260.00
产地: 美国(厂家直采)
公司:
产品分类:
公司分类: hydroxybenzene
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameAlphaSynucleinProtein
Description

HumanRecombinantAlphaSynucleinProteinAggregate(Control)

ApplicationsWB,SDS-PAGE,Invivoassay,Invitroassay
ConcentrationLot/batchspecific.Seeincludeddatasheet.
ConjugatesNotag

Properties

StorageBuffer0.2µmfilteredsolutionin20mMtris,150mMNaClpH7.5
StorageTemperature-80ºC
ShippingTemperatureBlueIceor4ºC
PurificationIon-exchangePurified
Specificity~14.46kDa
CiteThisProductHumanRecombinantAlphaSynucleinProtein(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SPR-317)
CertificateofAnalysisCertified92%pureusingSDS-PAGEanalysis.

BIOLOGicalDescription

AlternativeNamesAlphasynucleinpre-formedfibrils,Alphasynucleinaggregates,Alphasynucleinproteinaggregates,Alphasynucleinaggregates,Alpha-synucleinprotein,Non-AbetacomponentofADamyloidprotein,Non-A4componentofamyloidprecursorprotein,NACPprotein,SNCAprotein,NACPprotein,PARK1protein,SYNprotein,Parkisondiseasefamilial1Protein
ResearchAreasAlzheimer"sDisease,Neurodegeneration,Neuroscience,Parkinson"sDisease
CellularLocalizationCytoplasm,Membrane,Nucleus
AccessionNumberNP_000336.1
GeneID6622
SwissProtP37840
ScientificBackgroundAlpha-Synuclein(SNCA)isexpressedpredominantlyinthebrain,whereitisconcentratedinpresynapticnerveterminals(1).Alpha-synucleinishighlyexpressedinthemitochondriaoftheolfactorybulb,hippocampus,striatumandthalamus(2).Functionally,ithasbeenshowntosignificantlyinteractwithtubulin(3),andmayserveasapotentialmicrotubule-associatedprotein.Ithasalsobeenfoundtobeessentialfornormaldevelopmentofthecognitivefunctions;inactivationmayleadtoimpairedspatiallearningandworkingmemory(4).SNCAfibrillaraggregatesrepresentthemajornonA-betacomponentofAlzheimersdiseaseamyloidplaque,andamajorcomponentofLewybodyinclusions,andParkinson’sdisease.Parkinson"sdisease(PD)isacommonneurodegenerativedisordercharacterizedbytheprogressiveaccumulationinselectedneuronsofproteininclusionscontainingalpha-synucleinandubiquitin(5,6).
References1.“GeneticsHomeReference:SNCA”.USNationalLibraryofMedicine.(2013).
2.ZhangL.,etal.(2008)BrainRes.1244:40-52.
3.AlimM.A.,etal.(2002)JBiolChem.277(3):2112-2117.
4.KokhanV.S.,AfanasyevaM.A.,Van’kinG.(2012)Behav.Brain.Res.231(1):226-230.
5.SpillantiniM.G.,etal.(1997)Nature.388(6645):839-840.
6.MezeyE.,etal.(1998)NatMed.4(7):755-757.

ProductImages

<p>SDS-PAGE of ~14 kDa Human Recombinant Alpha Synuclein Protein Aggregate (Control) (SPR-317). Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate (SPR-317).</p>

SDS-PAGEof~14kDaHumanRecombinantAlphaSynucleinProteinAggregate(Control)(SPR-317).Lane1:MolecularWeightLadder(MW).Lane2:AlphaSynucleinProteinAggregate(SPR-317).

<p>Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 µg/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate (SPR-317) at 4 µg/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde made from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.</p>

PrimaryrathippocampalneuronsshowlewybodyinclusionformationwhentreatedwithactiveAlphaSynucleinProteinAggregate(SPR-322)at4µg/ml(D-F),butnotwhentreatedwithcontrolAlphaSynucleinProteinAggregate(SPR-317)at4µg/ml(A-C).Tissue:Primaryhippocampalneurons.Species:Sprague-Dawleyrat.Fixation:4%formaldehydemadefromPFA.PrimaryAntibody:Mouseanti-pSer129Antibodyat1:100024hoursat4°C.SecondaryAntibody:FITCGoatAnti-Mouse(green)at1:700for1hoursatRT.Counterstain:Hoechst(blue)nuclearstainat1:4000for1houratRT.Localization:Lewybodyincluscions.Magnification:20x.

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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。